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Archives of virology1990; 111(3-4); 199-212; doi: 10.1007/BF01311054

Equine infectious anemia virus (EIAV) humoral responses of recipient ponies and antigenic variation during persistent infection.

Abstract: Three ponies were inoculated with plasma containing 10(4.8) TCID50 of equine infectious anemia virus (EIAV) and observed for 165 to 440 days. Each pony developed a febrile response within 3 weeks of infection during which a plasma viremia greater than or equal to 10(3.5) TCID50/ml was observed. Analyses of four isolates from sequential febrile episodes in a single pony were conducted by two-dimensional tryptic peptide maps and with monoclonal antibodies in immunoblots. Structural and antigenic alterations were observed in the envelope glycoproteins gp90 and gp45, with greatest variation in gp90. Specific IgG to EIAV gp90, gp45, and p26 of homologous and heterologous isolates was detectable by immunoblots within one month after infection although IgG levels to gp45 at this time were relatively low. The group-specific determinants of gp90 and gp45 were more antigenic than those of p26; however, binding of IgG to these determinants did not correlate with neutralization of EIAV as assayed in fetal equine kidney cells. Neutralizing antibodies were first detectable within two months of infection and only neutralized viruses isolated prior to serum collection. Neutralizing activity of sera collected later in the infection was broadly reactive regardless of the number of clinical episodes the donor had suffered.
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Publication Date: 1990-01-01 PubMed ID: 2162160DOI: 10.1007/BF01311054Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the response of ponies to the equine infectious anemia virus (EIAV) over a time period of up to 440 days. The focus is mainly on the changes in the structure and antigens of the virus’ glycoproteins and their impact on the infected ponies’ immune responses.

Experiment and Observations

  • The researchers began by injecting three ponies with a plasma containing a specific amount of equine infectious anemia virus.
  • The ponies were observed for a period ranging from 165 to 440 days, and within three weeks of infection, each pony showed signs of fever. It was observed that more than or equal to 10(3.5) TCID50/ml of the virus was found in the pony’s plasma during this period.
  • In one pony, researchers analyzed four isolates from sequential bouts of fever by creating two-dimensional tryptic peptide maps and using monoclonal antibodies in immunoblots.

Virus Structural and Antigenic Alterations

  • During the analysis, it was evident that there were structural and antigenic alterations in the glycoproteins, gp90 and gp45, of EIAV. Maximum variation was observed in gp90.
  • The specific IgG antibodies against EIAV’s gp90, gp45, and p26 proteins in both homologous (same species) and heterologous (different species) isolates were detectable within a month after infection by immunoblots.
  • The IgG levels for gp45 were relatively low at this time in the infection.

Antigenic Specifics and EIAV Neutralization

  • The group-specific determinants (distinctive features which prompt an immune response) of gp90 and gp45 were more antigenic (elicited a stronger immune response) than those of p26.
  • However, a positive correlation was not found between the binding of IgG to these determinants and EIAV neutralization.
  • Neutralizing antibodies were first detectable within two months of infection, but they only neutralized viruses isolated before the collection of the serum.
  • Sera collected later in the infection, regardless of the number of clinical episodes suffered by the donor, showed a broad reactivity in neutralizing the virus.

Cite This Article

APA
Rwambo PM, Issel CJ, Adams WV, Hussain KA, Miller M, Montelaro RC. (1990). Equine infectious anemia virus (EIAV) humoral responses of recipient ponies and antigenic variation during persistent infection. Arch Virol, 111(3-4), 199-212. https://doi.org/10.1007/BF01311054

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 111
Issue: 3-4
Pages: 199-212

Researcher Affiliations

Rwambo, P M
  • Department of Veterinary Microbiology and Parasitology, Louisiana State University, School of Veterinary Medicine, Baton Rouge.
Issel, C J
    Adams, W V
      Hussain, K A
        Miller, M
          Montelaro, R C

            MeSH Terms

            • Animals
            • Antibodies, Monoclonal
            • Antibodies, Viral / biosynthesis
            • Antibodies, Viral / immunology
            • Antibody Specificity
            • Antigenic Variation
            • Antigens, Viral / immunology
            • Chronic Disease
            • Equine Infectious Anemia / immunology
            • Equine Infectious Anemia / microbiology
            • Glycoproteins / immunology
            • Horse Diseases / immunology
            • Horse Diseases / microbiology
            • Horses
            • Immunoblotting / veterinary
            • Infectious Anemia Virus, Equine / immunology
            • Neutralization Tests / veterinary
            • Peptide Mapping / veterinary
            • Protein Conformation
            • Viral Envelope Proteins / immunology

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            Citations

            This article has been cited 23 times.
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