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Home / Equine Research Bank / J Virol / Equine infectious anemia virus gene expression: characteriza...
Journal of virology1992; 66(6); 3455-3465; doi: 10.1128/JVI.66.6.3455-3465.1992

Equine infectious anemia virus gene expression: characterization of the RNA splicing pattern and the protein products encoded by open reading frames S1 and S2.

Abstract: The utilization of predicted splice donor and acceptor sites in generating equine infectious anemia virus (EIAV) transcripts in fetal donkey dermal cells (FDD) was examined. A single splice donor site identified immediately upstream of the gag coding region joins the viral leader sequence to all downstream exons of spliced EIAV transcripts. The predominant 3.5-kb transcript synthesized in EIAV-infected FDD cells appears to be generated by a single splicing event which links the leader sequence to the first of two functional splice acceptor sites near the 5' end of the S1 open reading frame (ORF). The translation products encoded by the 3.5-kb transcript were examined by producing in vitro transcripts from a cDNA corresponding to this RNA followed by in vitro translation in wheat germ extracts. These transcripts directed the synthesis of three proteins: the virus trans-activator protein (EIAV Tat) encoded by ORF S1, a protein of unknown function encoded by ORF S2, and the virus envelope glycoprotein. When transfected into FDD cells, this cDNA also directed expression of EIAV Tat. Amino-terminal sequence analysis of the in vitro-synthesized S1 protein supports the suggestion that translation of EIAV Tat is initiated at a CUG codon within the virus leader region. Both in vitro-synthesized S2 protein and synthetic peptides corresponding to S2 are shown to react positively with sera obtained from EIAV-infected horses, providing the first direct evidence of expression of this protein in infected animals.
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Publication Date: 1992-06-01 PubMed ID: 1316461PubMed Central: PMC241126DOI: 10.1128/JVI.66.6.3455-3465.1992Google Scholar: Lookup
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  • Non-U.S. Gov't
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  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on understanding the gene expression patterns in equine infectious anemia virus (EIAV) through examining RNA splicing in fetal donkey dermal cells.

Examination of RNA Splicing

  • The study looked at how predicted splice donor and acceptor sites generate EIAV transcripts in fetal donkey dermal cells (FDD).
  • A single splice donor site was found near the gag coding region, connecting the viral leader sequence to all downstream exons of the EIAV transcripts.

Transcript Synthesis and Translation Products

  • The main 3.5-kb transcript appears to be created via a single splicing event linking the leader sequence to the first functional splice acceptor site near the beginning of the S1 open reading frame (ORF).
  • Three proteins were identified as the translation products of the 3.5-kb transcript: the EIAV trans-activator protein (EIAV Tat) encoded by ORF S1, an unknown protein encoded by ORF S2, and the virus envelope glycoprotein.

Results from Transfection and Analysis

  • When cDNA corresponding to the 3.5-kb transcript was transfected into FDD cells, it led to the expression of EIAV Tat, confirming the role of this genetic sequence.
  • Amino-terminal sequence analysis of the in-vitro-synthesized S1 protein suggests that translation of EIAV Tat begins at a specific CUG codon within the viral leader region.

Evidence of S2 Protein Expression in Infected Animals

  • Both the in-vitro-synthesized S2 protein and synthetic peptides corresponding to S2 showed positive reactions to sera derived from EIAV-infected horses. This presents the first direct proof of expression of the S2 protein in infected animals.

Cite This Article

APA
Schiltz RL, Shih DS, Rasty S, Montelaro RC, Rushlow KE. (1992). Equine infectious anemia virus gene expression: characterization of the RNA splicing pattern and the protein products encoded by open reading frames S1 and S2. J Virol, 66(6), 3455-3465. https://doi.org/10.1128/JVI.66.6.3455-3465.1992

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 66
Issue: 6
Pages: 3455-3465

Researcher Affiliations

Schiltz, R L
  • Department of Biochemistry, Louisiana State University, Baton Rouge 70803.
Shih, D S
    Rasty, S
      Montelaro, R C
        Rushlow, K E

          MeSH Terms

          • Amino Acid Sequence
          • Animals
          • Base Sequence
          • Cells, Cultured
          • Cloning, Molecular
          • Gene Expression Regulation, Viral
          • Infectious Anemia Virus, Equine / genetics
          • Infectious Anemia Virus, Equine / metabolism
          • Molecular Sequence Data
          • Oligonucleotide Probes
          • Perissodactyla
          • RNA Precursors / genetics
          • RNA Splicing
          • RNA, Messenger / metabolism
          • Single-Strand Specific DNA and RNA Endonucleases / metabolism
          • Transcription, Genetic
          • Transcriptional Activation
          • Viral Envelope Proteins / biosynthesis

          Grant Funding

          • CA49296 / NCI NIH HHS

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