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Home / Equine Research Bank / Immunology / Equine monoclonal antibodies recognize common epitopes on va...
Immunology1990; 71(4); 592-594;

Equine monoclonal antibodies recognize common epitopes on variants of equine infectious anaemia virus.

Abstract: Equine-murine xenohybridoma cells were produced using SP2/0 murine myeloma cells and splenic lymph node cells obtained from horses infected with 10(6) TCID50 of single cloned variants of equine infectious anaemia virus (EIAV). The xenohybridomas secreted equine IgG monoclonal antibodies reactive with EIAV in enzyme immunoassays employing purified virus. Seven antibodies were studied in detail. They bound to viral glycoproteins (gp90 or gp45) in radioimmunoprecipitation assays, and reacted with homologous EIAV as well as five other cloned variants of EIAV. When evaluated against a single cloned variant of EIAV (EIAV-WSU5), two antibodies bound to different epitopes on gp90. The five remaining antibodies reacted with the same or overlapping epitopes on gp45. None of the antibodies exhibited viral neutralizing activity.
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Publication Date: 1990-12-01 PubMed ID: 1703988PubMed Central: PMC1384884
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study focuses on creating equine antibodies that can identify common aspects of various strains of equine infectious anaemia virus, using xenohybridoma cells derived from horses and mice.

Creation of Xenohybridomas

  • The study produced xenohybridoma cells, which are hybrid cells combining equine (horse) and murine (mouse) elements. This was achieved by using the SP2/0 mouse myeloma cells and cells from the splenic lymph nodes of horses, which were infected with large quantities (10(6) TCID50) of individually cloned versions of EIAV.

Production of Equine Monoclonal Antibodies

  • The newly created xenohybridomas secreted equine IgG monoclonal antibodies. Researchers found that these antibodies reacted positively with EIAV when tested in enzyme immunoassays that utilized purified virus—implying these monoclonal antibodies have the capability to recognize and bind to the EIAV virus.

Antibody Interaction with Viral Glycoproteins

  • Scientists utilized radioimmunoprecipitation assays to analyze how the antibodies interacted with viral glycoproteins (proteins situated on the virus’ outer surface). They found that the antibodies adhered to two specific glycoproteins: gp90 and gp45.

Reactivity with Various EIAV Variants

  • The antibodies reacted not only with the specific EIAV variant they were derived from (homologous EIAV) but also with five other individually cloned EIAV variants. This implies a broad reactivity, as they can recognize common characteristics on multiple forms of the virus.

Precise Antibody Attachment Points

  • When tested against a specific variant of EIAV (EIAV-WSU5), two of the antibodies attached themselves to different areas (epitopes) on the gp90 glycoprotein. The other five antibodies bound to the same, or overlapping points on the gp45 glycoprotein.

Lack of Viral Neutralizing Activity

  • None of the seven antibodies studied demonstrated the ability to neutralize the virus. This means while these antibodies can recognize and bind to EIAV, they lack the ability to disable or destroy the virus.

Cite This Article

APA
Perryman LE, O'Rourke KI, Mason PH, McGuire TC. (1990). Equine monoclonal antibodies recognize common epitopes on variants of equine infectious anaemia virus. Immunology, 71(4), 592-594.

Publication

Immunology
ISSN: 0019-2805
NlmUniqueID: 0374672
Country: England
Language: English
Volume: 71
Issue: 4
Pages: 592-594

Researcher Affiliations

Perryman, L E
  • Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
O'Rourke, K I
    Mason, P H
      McGuire, T C

        MeSH Terms

        • Animals
        • Antibodies, Monoclonal
        • Antigens, Viral / analysis
        • Enzyme-Linked Immunosorbent Assay
        • Epitopes / analysis
        • Horses
        • Hybridomas / immunology
        • Infectious Anemia Virus, Equine / immunology
        • Mice
        • Radioimmunoprecipitation Assay

        Grant Funding

        • AI 24166 / NIAID NIH HHS
        • AI 24291 / NIAID NIH HHS

        References

        This article includes 19 references
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        Citations

        This article has been cited 3 times.
        1. Lonning SM, Zhang W, McGuire TC. Gag protein epitopes recognized by CD4(+) T-helper lymphocytes from equine infectious anemia virus-infected carrier horses.. J Virol 1999 May;73(5):4257-65.
          doi: 10.1128/JVI.73.5.4257-4265.1999pubmed: 10196322google scholar: lookup
        2. Lonning SM, Zhang W, Leib SR, McGuire TC. Detection and induction of equine infectious anemia virus-specific cytotoxic T-lymphocyte responses by use of recombinant retroviral vectors.. J Virol 1999 Apr;73(4):2762-9.
          doi: 10.1128/JVI.73.4.2762-2769.1999pubmed: 10074123google scholar: lookup
        3. McGuire TC, Tumas DB, Byrne KM, Hines MT, Leib SR, Brassfield AL, O'Rourke KI, Perryman LE. Major histocompatibility complex-restricted CD8+ cytotoxic T lymphocytes from horses with equine infectious anemia virus recognize Env and Gag/PR proteins.. J Virol 1994 Mar;68(3):1459-67.
          doi: 10.1128/JVI.68.3.1459-1467.1994pubmed: 8107209google scholar: lookup
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