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Home / Equine Research Bank / Vet Pathol / Equine multinodular pulmonary fibrosis: a newly recognized h...
Veterinary pathology2007; 44(6); 849-862; doi: 10.1354/vp.44-6-849

Equine multinodular pulmonary fibrosis: a newly recognized herpesvirus-associated fibrotic lung disease.

Abstract: Pulmonary fibrosis and interstitial lung disease are poorly understood in horses; the causes of such conditions are rarely identified. Equine herpesvirus 5 (EHV-5) is a gamma-herpesvirus of horses that has not been associated with disease in horses. Pathologic and virologic findings from 24 horses with progressive nodular fibrotic lung disease associated with EHV-5 infection are described and compared with 23 age-matched control animals. Gross lesions consisted of multiple nodules of fibrosis throughout the lungs. Histologically, there was marked interstitial fibrosis, often with preservation of an "alveolar-like" architecture, lined by cuboidal epithelial cells. The airways contained primarily neutrophils and macrophages. Rare macrophages contained large eosinophilic intranuclear viral inclusion bodies; similar inclusion bodies were also found cytologically. The inclusions were identified as herpesviral-like particles by transmission electron microscopy in a single horse. In situ hybridization was used to detect EHV-5 nucleic acids within occasional macrophage nuclei. With polymerase chain reaction (PCR), the herpesviral DNA polymerase gene was detected in 19/24 (79.2%) of affected horses and 2/23 (8.7%) of the control horses. Virus genera-specific PCR was used to detect EHV-5 in all of the affected horses and none of the control horses. EHV-2 was detected in 8/24 (33.3%) of affected horses and 1/9 (11.1%) of the control horses. This disease has not been reported before, and the authors propose that based upon the characteristic gross and histologic findings, the disease be known as equine multinodular pulmonary fibrosis. Further, we propose that this newly described disease develops in association with infection by the equine gamma-herpesvirus, EHV-5.
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Publication Date: 2007-11-28 PubMed ID: 18039898DOI: 10.1354/vp.44-6-849Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper discusses a fibrotic lung disease in horses associated with infection of the Equine herpesvirus 5 (EHV-5), never reported before, named as equine multinodular pulmonary fibrosis.

Objective and Methodology

  • The goal of this research was to study the pulmonary fibrosis and interstitial lung disease in horses which are generally not well-understood.
  • The researchers analyzed pathological and microbiological findings from 24 horses with progressive nodular fibrotic lung disease linked to EHV-5 infection.
  • The findings were compared with 23 age-matched control animals.

Main Findings

  • Pathologically, the diseased horses displayed multiple nodules of fibrosis throughout the lungs.
  • Upon microscopical examination, there existed marked interstitial fibrosis, often with the preservation of an “alveolar-like” architecture.
  • The walls of the airways were primarily lined with neutrophils and macrophages.
  • Some of the macrophages possessed large pink intranuclear viral inclusion bodies.
  • Herpesviral-like particles were spotted in a single horse using transmission electron microscopy.

Virus Detection

  • EHV-5 nucleic acids were detected within occasional macrophage nuclei using a technique called in situ hybridization.
  • The herpesvirus DNA polymerase gene was detected in 79.2% of affected horses and only 8.7% of control horses using a technique called polymerase chain reaction (PCR).
  • A Virus genera-specific PCR technique detected EHV-5 in all affected horses and in none of the control horses.
  • EHV-2 was detected in 33.3% of affected horses and 11.1% of control horses.

Conclusion

  • The authors concluded that based on the distinct gross and histologic findings, this disease should be referred to as equine multinodular pulmonary fibrosis.
  • Furthermore, this newly identified disease might be developing in conjunction with an infection by EHV-5, a species of equine gamma-herpesvirus.

Cite This Article

APA
Williams KJ, Maes R, Del Piero F, Lim A, Wise A, Bolin DC, Caswell J, Jackson C, Robinson NE, Derksen F, Scott MA, Uhal BD, Li X, Youssef SA, Bolin SR. (2007). Equine multinodular pulmonary fibrosis: a newly recognized herpesvirus-associated fibrotic lung disease. Vet Pathol, 44(6), 849-862. https://doi.org/10.1354/vp.44-6-849

Publication

Veterinary pathology
ISSN: 0300-9858
NlmUniqueID: 0312020
Country: United States
Language: English
Volume: 44
Issue: 6
Pages: 849-862

Researcher Affiliations

Williams, K J
  • College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA. williamsk@dcpah.msu.edu
Maes, R
    Del Piero, F
      Lim, A
        Wise, A
          Bolin, D C
            Caswell, J
              Jackson, C
                Robinson, N E
                  Derksen, F
                    Scott, M A
                      Uhal, B D
                        Li, X
                          Youssef, S A
                            Bolin, S R

                              MeSH Terms

                              • Animals
                              • Female
                              • Herpesviridae Infections / pathology
                              • Herpesviridae Infections / veterinary
                              • Herpesviridae Infections / virology
                              • Horse Diseases / pathology
                              • Horse Diseases / virology
                              • Horses
                              • Immunohistochemistry
                              • Lung / pathology
                              • Lung / ultrastructure
                              • Male
                              • Polymerase Chain Reaction
                              • Pulmonary Fibrosis / pathology
                              • Pulmonary Fibrosis / veterinary
                              • Pulmonary Fibrosis / virology
                              • Varicellovirus / isolation & purification
                              • Varicellovirus / ultrastructure

                              Citations

                              This article has been cited 35 times.
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