Equine oocyte in vitro maturation: influences of sera, time, and hormones.
Abstract: Objectives of the present research were to determine the influences of types of media, sera, time and hormones on equine oocyte in vitro maturation (IVM). The following types of media and sera were evaluated: Menezo's B2 medium (B2), modified Tissue Culture Medium 199 (TCM), Defined Medium (DM), fetal calf serum (FCS), mare serum collected on the first day of estrus (MS), and mare serum collected on the day of ovulation (MSO). Resultant oocyte maturation was compared with the control: DM with bovine serum albumin (BSA). Effect of culture time (0, 15, and 32 hr) and the following hormones on oocyte IVM were evaluated: none, bovine luteinizing hormone (bLH; 1, 10, 100 micrograms/ml), equine luteinizing hormone (eLH; 100 micrograms/ml), bovine follicle-stimulating hormone (FSH; 5 micrograms/ml), and equine chorionic gonadotropin (eCG; 1 and 100 IU/ml). Cumulus expansion in the media and sera experiments was 50% (DM with BSA), 80% (TCM, B2, and DM with MS or MSO), and 100% (FCS with any medium). The proportion of metaphase II (MII) oocytes was significantly (P less than 0.05) increased the percentage of MII oocytes as compared with 0 hr of culture. Cumulus expansion in the hormone experiments was 80% (none, bLH, and eLH), and 100% (eCG and FSH). Freshly prepared bLH significantly (P less than 0.05) inhibited nuclear maturation of equine oocytes. In summary, 15 hr of culture was sufficient time for equine oocyte IVM and all combinations of medium, serum, and hormone addition were equally effective in achieving IVM except fresh bLH and DM with BSA.
Publication Date: 1991-12-01 PubMed ID: 1751041DOI: 10.1002/mrd.1080300411Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research aimed to study the effects of different types of media, sera, time and hormones on the in vitro maturation (IVM) of horse oocytes (egg cells). They found that all combinations were effective except for freshly prepared bovine luteinizing hormone (bLH) and a specific medium with bovine serum albumin (BSA), and that 15 hours of culture was sufficient time for IVM.
Study Design and Methods
- The study examined the effects of different types of media including Menezo’s B2 medium (B2), modified Tissue Culture Medium 199 (TCM), and Defined medium (DM).
- Different types of sera were also tested, including fetal calf serum (FCS), mare serum collected on the first day of estrus (MS), and mare serum collected on the day of ovulation (MSO).
- The researchers then compared the oocyte maturation results from the test combinations to a control: Defined Medium (DM) with bovine serum albumin (BSA).
- The study also tested the effects of culture time (0, 15, and 32 hours) and different hormones (none, bovine and equine luteinizing hormone, bovine follicle-stimulating hormone, and equine chorionic gonadotropin) on oocyte IVM.
Results
- In media and sera experiments, the expansion of cumulus cells surrounding the oocyte was 50% in DM with BSA, 80% in TCM, B2, and DM with MS or MSO, while full expansion (100%) was seen with FCS in any medium.
- The research found that the proportion of mature oocytes (metaphase II or M2 oocytes) significantly increased compared to zero hours of culture.
- With hormone combinations, cumulus expansion was 80% with no added hormone, bLH, and eLH, and 100% with eCG and FSH.
- In a noteworthy finding, freshly prepared bLH significantly inhibited nuclear maturation of horse oocytes.
Summary
- According to the research, 15 hours appears to be the optimal culture time for equine oocyte in vitro maturation.
- All combinations of medium, serum, and hormone addition were found to be equally effective in achieving IVM, with the exception of fresh bLH and DM combined with BSA.
This research helps to enhance our understanding of the optimal conditions for equine oocyte IVM, which may be beneficial for increasing success rates in horse breeding and conservation efforts.
Cite This Article
APA
Willis P, Caudle AB, Fayrer-Hosken RA.
(1991).
Equine oocyte in vitro maturation: influences of sera, time, and hormones.
Mol Reprod Dev, 30(4), 360-368.
https://doi.org/10.1002/mrd.1080300411 Publication
Researcher Affiliations
- Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
MeSH Terms
- Animals
- Blood
- Cells, Cultured
- Chorionic Gonadotropin / pharmacology
- Culture Media / pharmacology
- Female
- Follicle Stimulating Hormone / pharmacology
- Hormones / pharmacology
- Horses
- Luteinizing Hormone / pharmacology
- Oocytes / cytology
- Oogenesis
- Time Factors
Citations
This article has been cited 4 times.- Abdoon AS, Abdel-Rahman HA, Shawki SM, Kandil OM, Fathalla SI. Influence of follicle size, methods of retrieval on oocytes yield and morphology in Egyptian Jennies ovaries with special reference to maturation rate in vitro. Vet Res Commun 2014 Dec;38(4):287-95.
- Shirazi A, Ardali MA, Ahmadi E, Nazari H, Mamuee M, Heidari B. The Effect of Macromolecule Source and Type of Media During in vitro Maturation of Sheep Oocytes on Subsequent Embryo Development. J Reprod Infertil 2012 Jan;13(1):13-9.
- Lange Consiglio A, Dell'Aquila ME, Fiandanese N, Ambruosi B, Cho YS, Bosi G, Arrighi S, Lacalandra GM, Cremonesi F. Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse. Reprod Biol Endocrinol 2009 Oct 16;7:113.
- Dell'Aquila ME, Caillaud M, Maritato F, Martoriati A, Gérard N, Aiudi G, Minoia P, Goudet G. Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis. Reprod Biol Endocrinol 2004 Jun 22;2:44.
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