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Equine veterinary journal2012; 44(5); 600-605; doi: 10.1111/j.2042-3306.2011.00536.x

Equine peripheral blood-derived mesenchymal stem cells: isolation, identification, trilineage differentiation and effect of hyperbaric oxygen treatment.

Abstract: Two studies report variability in proliferation and limited adipocyte differentiation of equine peripheral blood-derived adult mesenchymal stem cells, thus casting doubt on their adipogenic potential. Peripheral blood can be a valuable source of adult mesenchymal stem cells if cell culture conditions permissive for their adherence, proliferation and differentiation are defined. Hyperbaric oxygen treatment has been reported to mobilise haematopoietic progenitor stem cells into the peripheral blood in humans and mice, but similar experiments have not been done in horses. Objective: To optimise cell culture conditions for isolation, propagation and differentiation of adult stem cells from peripheral blood and to assess the effect of hyperbaric oxygen treatment on adult stem cell concentrations. Methods: Peripheral blood was collected from the jugular vein of 6 research mares, and mononuclear cells were isolated. They were subjected to cell culture conditions that promote the adherence and proliferation of adult stem cells. The cells were characterised by their adherence, expression of cellular antigen markers, and trans-differentiation. Each horse was subjected to 3 hyperbaric oxygen treatments, and stem cells were compared before and after treatments. Stem cells derived from adipose tissue were used as controls. Results: One-third of the horses yielded viable stem cells from peripheral blood, positive for CD51, CD90 and CD105, and demonstrated osteocyte, chondrocyte and adipocyte differentiation. Hyperbaric oxygen treatment resulted in a significant increase in CD90-positive cells. Horses that did not yield any cells pretreatment did so only after 3 hyperbaric oxygen treatments. Conclusions: Peripheral blood can be a valuable source of adult stem cells, if one can identify reliable equine-specific markers, provide methods to increase the number of circulating progenitor cells and optimise cell culture conditions for growth and viability. Our findings are important for further studies towards technological advances in basic and clinical equine regenerative medicine.
© 2012 EVJ Ltd.
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Publication Date: 2012-02-15 PubMed ID: 22333000DOI: 10.1111/j.2042-3306.2011.00536.xGoogle Scholar: Lookup
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  • Clinical Trial
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the isolation, identification, and differentiation of adult stem cells derived from equine peripheral blood. Additionally, it explores the effect of hyperbaric oxygen treatments on the concentration of these stem cells.

Study Overview

  • The study objective was to optimize cell culture conditions for isolating, propagating, and differentiating adult stem cells from peripheral blood, and to measure the impact of hyperbaric oxygen treatment on adult stem cell concentrations.
  • Research was conducted using the blood of 6 research mares, and aimed to characterize cells based on their adherence, expression of cellular antigen markers, and trans-differentiation capabilities.
  • Furthermore, each horse was subjected to three hyperbaric oxygen treatments with stem cells compared before and after these treatments.
  • For comparison and control purposes, stem cells derived from adipose tissue were used.

Study Findings

  • The team found that a third of the horses yielded viable stem cells from peripheral blood, with these cells demonstrating positive results for CD51, CD90, and CD105 markers, and evidencing the ability to differentiate into osteocytes, chondrocytes, and adipocytes.
  • Importantly, hyperbaric oxygen treatment resulted in a significant increase in CD90-positive cells.
  • Even more interesting was the revelation that horses which did not yield any cells prior to the treatment, did so only after undergoing the three hyperbaric oxygen treatments.

Conclusion and Implication

  • The researchers concluded that peripheral blood could be a valuable source of adult stem cells, contingent on the identification of reliable equine-specific markers and the optimization of cell culture conditions that encourage growth and viability.
  • Additionally, increasing the number of circulating progenitor cells was identified as another crucial factor.
  • These findings are significant for further studies in basic and clinical equine regenerative medicine and could aid in the development of new technologies for treating various equine diseases.

Cite This Article

APA
Dhar M, Neilsen N, Beatty K, Eaker S, Adair H, Geiser D. (2012). Equine peripheral blood-derived mesenchymal stem cells: isolation, identification, trilineage differentiation and effect of hyperbaric oxygen treatment. Equine Vet J, 44(5), 600-605. https://doi.org/10.1111/j.2042-3306.2011.00536.x

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 44
Issue: 5
Pages: 600-605

Researcher Affiliations

Dhar, M
  • Departments of Large Animal Clinical Sciences Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville, TN, USA. mdhar@utk.edu
Neilsen, N
    Beatty, K
      Eaker, S
        Adair, H
          Geiser, D

            MeSH Terms

            • Adipose Tissue / cytology
            • Animals
            • Antigens, CD / genetics
            • Antigens, CD / metabolism
            • Cell Adhesion / physiology
            • Cell Differentiation
            • Cells, Cultured
            • Female
            • Gene Expression Regulation / physiology
            • Horses / blood
            • Hyperbaric Oxygenation
            • Mesenchymal Stem Cells / physiology

            Citations

            This article has been cited 22 times.
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