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Home / Equine Research Bank / Front Vet Sci / Equine Transport and Changes in Equid Herpesvirus’ Sta...
Frontiers in veterinary science2018; 5; 224; doi: 10.3389/fvets.2018.00224

Equine Transport and Changes in Equid Herpesvirus’ Status.

Abstract: The risk of respiratory disease in the transported horse can increase as a consequence of immunosuppression and stress associated primarily with opportunistic bacterial proliferation and viral reactivation. This study examines the ecology of equid herpesviruses (EHV) in these horses, exploring reactivation and changes in infection and shedding associated with transport, and any potential contributions to transport-related respiratory disease. Twelve horses were subjected to an 8-h road-transport event. Antibodies to EHV-1 and EHV-4 were detected by ELISA in serum collected prior to, immediately after and 2 weeks post transport. Respiratory tract endoscopy and tracheal washes were collected prior to and 5 days after transportation. Nasal swabs collected prior to, immediately after, 1 and 5 days following transport were screened for EHV-1,-2,-4,-5 using qPCR. Six horses had persistent neutrophilic airway infiltrates post transportation, indicative of subclinical respiratory disease. No horses were qPCR positive for either of the alphaherpesviruses (i.e., EHV-1/-4) nor did any seroconvert to either virus. Four out of nine horses positive for either EHV-2 or EHV-5 on qPCR prior to transport developed neutrophilic airway inflammation. Five horses showed increasingly positive readings on qPCR (i.e., reduced Cq) for EHV-2 after transportation and seven out of eleven horses positive for EHV-2 after transport shared strains of high sequence similarity with other horses in the study. One EHV-2 virus detected in one horse after transport was genetically different which may be due to reactivation. The clinical significance of EHV-2 and EHV-5 remains in question. However these results indicate that transportation may lead to increased shedding, transmission and reactivation of EHV-2 and EHV-5 but not EHV-1/-4. Unlike previous work focusing on the role of alphaherpesviruses, this research suggests that investigation of the gammaherpesviruses (i.e., EHV-2/-5) in transport-related disease should not be dismissed, particularly given that these viruses can encode suppressive immunomodulators that may affect host health.
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Publication Date: 2018-09-25 PubMed ID: 30320126PubMed Central: PMC6167981DOI: 10.3389/fvets.2018.00224Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the impact of transportation on horses’ health, particularly concerning the reactivation and progression of equid herpesviruses (EHV), which are potentially contributing to transport-related respiratory illnesses.

Objective of the Research

  • This study aims to examine the effect of transportation on the behaviour of equid herpesviruses (EHV) in horses. It seeks to understand EHV’s reactivation and how changes in its infection and shedding could contribute to transport-associated respiratory diseases.

Methodology

  • Twelve horses were subjected to an 8-hour road-transport event, and samples were then collected at various stages, including before, immediately after, and two weeks post-transport.
  • Serum samples were screened for antibodies against EHV-1 and EHV-4 using enzyme-linked immunosorbent assay (ELISA).
  • Respiratory tract endoscopy and tracheal washes were analysed before and 5 days after transport.
  • Nasal swabs, collected at key stages of the study, were tested for EHV-1, -2, -4, -5 using quantitative PCR.

Key Findings

  • Six out of twelve horses exhibited persistent neutrophilic airway infiltrates after transport, indicative of subclinical respiratory disease.
  • None of the horses tested positive for alphaherpesviruses (EHV-1/-4), nor was there any seroconversion observed.
  • Four out of nine horses testing positive for either EHV-2 or EHV-5 prior to transport developed neutrophilic airway inflammation.
  • Transportation increased the shedding and reactivation of EHV-2 and EHV-5 but not EHV-1/-4.
  • The detected EHV-2 and EHV-5 strains shared high sequence similarity amongst transported horses, suggesting transmission via transport.
  • One horse developed a genetically different strain of EHV-2 post-transport, possibly the result of reactivation.

Conclusion

  • The importance of EHV-2 and EHV-5 in transport-related diseases cannot be overlooked, given that these viruses can produce suppressive immunomodulators potentially affecting host health.
  • The study does not fully clarify the clinical significance of EHV-2 and EHV-5 but highlights the transportation-related increase in virus shedding, transmission, and reactivation.
  • Unlike previous studies that chiefly focused on alphaherpesviruses, this research emphasizes the need to investigate gammaherpesviruses’ role (EHV-2 and EHV-5) in transport-related illnesses.

Cite This Article

APA
Muscat KE, Padalino B, Hartley CA, Ficorilli N, Celi P, Knight P, Raidal S, Gilkerson JR, Muscatello G. (2018). Equine Transport and Changes in Equid Herpesvirus’ Status. Front Vet Sci, 5, 224. https://doi.org/10.3389/fvets.2018.00224

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 5
Pages: 224
PII: 224

Researcher Affiliations

Muscat, Katharine E
  • School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, NSW, Australia.
Padalino, Barbara
  • School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, NSW, Australia.
  • Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, Hong Kong.
  • HKSAR- Department of Veterinary Medicine, University of Bari, Bari, Italy.
Hartley, Carol A
  • Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, Australia.
Ficorilli, Nino
  • Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, Australia.
Celi, Pietro
  • Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, Australia.
  • DSM, Parsippany, NJ, United States.
Knight, Peter
  • Discipline of Biomedical Science, School of Medical Sciences, University of Sydney, Sydney, NSW, Australia.
Raidal, Sharanne
  • School of Animal and Veterinary Sciences, Charles Stuart University, Wagga Wagga, NSW, Australia.
Gilkerson, James R
  • Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, Australia.
Muscatello, Gary
  • School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, NSW, Australia.

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Citations

This article has been cited 8 times.
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