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Establishment and application of a 10-plex liquid bead array for the simultaneous rapid detection of animal species.
Abstract: Meat fraud and adulteration incidents occur frequently in almost all regions of the globe, especially with the increase in the world's population. To ensure the authenticity of meat products, we developed a 10-plex xMAP assay to simultaneously detect ten animal materials: bovine, caprine, poultry, swine, donkey, deer, horse, dog, fox and mink. Results: This method was investigated by analyzing DNA extracts from raw muscle, muscle mixtures, meat products and animal feeds. Our results indicated that the species of interest can be identified, differentiated and detected down to 1 g kg in binary mixtures or 0.01-0.001 ng of genomic DNA from specific species. Testing of 125 commercial samples showed a 97.4% coincidence rate with the method used in routine testing in our lab. Conclusions: These results indicated that the method established in this study could detect ten animal materials simultaneously within 3 h, which provides a new, useful tool for animal ingredient analysis in meat products and animal feeds. © 2019 Society of Chemical Industry.
© 2019 Society of Chemical Industry.
Publication Date: 2019-11-06 PubMed ID: 31584699DOI: 10.1002/jsfa.10042Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
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Summary
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The research is about the development and application of a 10-plex liquid bead array to detect the meat of ten different animal species in food products and animal feeds. It is aimed at curbing meat fraud and adulteration by ensuring the integrity of meat products.
Development of the Method
- The researchers developed a 10-plex xMAP (multiplex assay) technique. This method was designed to simultaneously detect the DNA of ten different animal species in food products and animal feeds.
- The targeted animals were: bovine (cattle), caprine (goats), poultry, swine (pigs), donkey, deer, horse, dog, fox, and mink.
Application and Testing of the Method
- The method was tested on DNA extracts from raw muscles, mixtures of muscles, meat products, and animal feeds.
- Results showed that the species of interest could be identified, differentiated and detected down to 1 gram per kilogram in binary mixtures, or 0.01 to 0.001 nanograms of specific genomic DNA from the species of interest.
Efficacy of the Method
- To ascertain the efficacy of this method, the team tested 125 commercial samples. The method showed a 97.4% coincidence rate with the method regularly used in the lab for these tests. This indicates a high degree of accuracy and reliability.
- This novel method is able to detect the presence of ten different animal species within a short time frame of three hours.
Significance of the Research
- The research provides a useful tool for ensuring the authenticity of meat products and animal feeds. This method ensures integrity in the meat industry, deterring meat fraud and adulteration.
- As it stands, this 10-plex bead array promises to be an essential tool in not just protecting consumers’ rights but also ensuring adherence to dietary laws and regulations.
- Given the global prevalence of meat fraud and adulteration, this technique is of significant value to the food industry, regulatory bodies, and consumers at large.
Cite This Article
APA
Mei M, Chen R, Gao X, Cao Y, Weng W, Duan Y, Tan X, Liu Z.
(2019).
Establishment and application of a 10-plex liquid bead array for the simultaneous rapid detection of animal species.
J Sci Food Agric, 100(1), 325-334.
https://doi.org/10.1002/jsfa.10042 Publication
Researcher Affiliations
- Technical Center, Guangzhou Customs District People's Republic of China, Guangzhou, China.
- Technical Center, Guangzhou Customs District People's Republic of China, Guangzhou, China.
- Department of Genetics, National Research Institute for Family Planning, Beijing, China.
- State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-Sen University, Guangzhou, China.
- Technical Center, Guangzhou Customs District People's Republic of China, Guangzhou, China.
- Technical Center, Guangzhou Customs District People's Republic of China, Guangzhou, China.
- State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-Sen University, Guangzhou, China.
- Technical Center, Guangzhou Customs District People's Republic of China, Guangzhou, China.
MeSH Terms
- Animal Feed / analysis
- Animals
- Cattle
- DNA, Mitochondrial / genetics
- Deer
- Dogs
- Food Contamination / analysis
- Foxes
- Goats
- Horses
- Meat Products / analysis
- Mink
- Nucleic Acid Amplification Techniques / instrumentation
- Nucleic Acid Amplification Techniques / methods
- Oligonucleotide Array Sequence Analysis / methods
- Poultry
- Swine
Grant Funding
- 2018GDK04 / the Science and Technology Research Project of the former Guangdong Entry-exit Inspection and Quarantine Bureau in China
- 201707010487 / the Science Research Program of Guangzhou city in China
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Citations
This article has been cited 2 times.- Zenke P, Zorkóczy OK, Lehotzky P, Ózsvári L, Pádár Z. Molecular Sexing and Species Detection of Antlered European Hunting Game for Forensic Purposes. Animals (Basel) 2022 Jan 20;12(3).
- Martuscelli M, Serio A, Capezio O, Mastrocola D. Safety, Quality and Analytical Authentication of ḥalāl Meat Products, with Particular Emphasis on Salami: A Review. Foods 2020 Aug 13;9(8).
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