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Home / Equine Research Bank / In Vitro Cell Dev Biol Anim / Establishment and characterization of Caspian horse fibrobla...
In vitro cellular & developmental biology. Animal2016; 53(4); 337-343; doi: 10.1007/s11626-016-0120-3

Establishment and characterization of Caspian horse fibroblast cell bank in Iran.

Abstract: Caspian horse, a rare horse breed found in 1965 by Louise Firouz in northern Iran, is a small horse which is reported to be in danger of extinction in its original homeland. There seems to be a great need to prevent extinction of this valuable horse. In this study, 51 fibroblast cell lines from Caspian horse ear marginal tissue were successfully established by sampling 60 horses using primary explant technique. Cells were authenticated and growth curve was plotted. According to results obtained, population doubling time (PDT) was calculated 23 ± 0.5 h for all cell lines. Multiplex polymerase chain reaction (multiplex PCR) revealed that cell lines had no cross-contamination with other species. Bacteria, fungi, and mycoplasma contamination were checked using standard methods such as PCR, direct culture, and Hoechst staining. In addition to providing a valuable source for genomic, postgenomic, and somatic cloning researches, the established cell lines would preserve Caspian horse genetic resources. It will also create an accessible database for researchers.
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Publication Date: 2016-12-30 PubMed ID: 28039621DOI: 10.1007/s11626-016-0120-3Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focusses on the establishment and characteristics of a fibroblast cell bank for the Caspian horse, a rare breed at risk of extinction, in Iran.

Background

  • This research is centered around the Caspian horse, a rare, small-sized breed discovered in northern Iran in 1965 by Louise Firouz. This breed is reported to be at risk of extinction in its native homeland.
  • The study aims to prevent the extinction of the Caspian horse by forming a cell bank committed to the breed.

Methodology

  • The researchers established 51 fibroblast cell lines from ear marginal tissue of 60 Caspian horses using the primary explant technique, an in vitro methodology for isolating cells in tissue culture.
  • The authenticity of cells and growth dynamics were ascertained, and a growth curve was plotted.
  • The researchers calculated the population doubling time (PDT) — the time required to double the size of a population of cells in culture — which was determined as approximately 23 hours for all cell lines.

Results and Significance

  • Multiplex Polymerase Chain Reaction (multiplex PCR) revealed no cross-contamination with other species in the cell lines, signifying the purity of the Caspian horse genetic material.
  • The viability of the cells was confirmed by checking for bacteria, fungi, and mycoplasma contamination using standard methods such as PCR, direct culture, and Hoechst staining, a nuclear staining method commonly used in cell biology to visualize cell nuclei.
  • This research will serve as a valuable resource for future genomic, postgenomic, and somatic cloning studies. The established cell lines will help to preservation of Caspian horse’s genetic resources.
  • The study also advocates the creation of an accessible database, useful for researchers engaging with the unique genetics of the Caspian horse, thereby contributing to the broader goals of conserving and understanding biodiverse species.

Cite This Article

APA
Daneshvar Amoli A, Mohebali N, Farzaneh P, Shahzadeh Fazeli SA, Nikfarjam L, Ashouri Movasagh S, Moradmand Z, Ganjibakhsh M, Nasimian A, Izadpanah M, Vakhshiteh F, Gohari NS, Masoudi NS, Farghadan M, Mohamadi Moghanjoghi S, Khalili M, Khaledi KJ. (2016). Establishment and characterization of Caspian horse fibroblast cell bank in Iran. In Vitro Cell Dev Biol Anim, 53(4), 337-343. https://doi.org/10.1007/s11626-016-0120-3

Publication

In vitro cellular & developmental biology. Animal
ISSN: 1543-706X
NlmUniqueID: 9418515
Country: Germany
Language: English
Volume: 53
Issue: 4
Pages: 337-343

Researcher Affiliations

Daneshvar Amoli, Abdolreza
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Mohebali, Nazanin
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Farzaneh, Parvaneh
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Shahzadeh Fazeli, Seyed Abolhassan
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
  • Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran.
Nikfarjam, Laleh
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Ashouri Movasagh, Sepideh
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Moradmand, Zahra
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Ganjibakhsh, Meysam
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Nasimian, Ahmad
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Izadpanah, Mehrnaz
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Vakhshiteh, Faezeh
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Gohari, Neda Sadat
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Masoudi, Najmeh Sadat
  • Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Farghadan, Maryam
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Mohamadi Moghanjoghi, Shiva
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
Khalili, Masoud
  • Iran Equestrian Federation, University of Applied Science and Technology, Tehran, Iran.
Khaledi, Kourosh J
  • Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran. Khaledi@ibrc.ir.
  • Department of Agriculture, Yadegar-e-Imam Khomeini (rah), Shahr-e- rey Branch, Islamic Azad university, Tehran, Iran. Khaledi@ibrc.ir.

MeSH Terms

  • Animals
  • Cell Line
  • Cell Proliferation
  • Cell Shape
  • Cell Survival
  • Chromosomes, Mammalian / genetics
  • Female
  • Fibroblasts / cytology
  • Horses
  • Immunohistochemistry
  • Iran
  • Male
  • Polymerase Chain Reaction
  • Tissue Banks
  • Transfection

References

This article includes 17 references
  1. Petersen JL, Mickelson JR, Cothran EG, Andersson LS, Axelsson J, Bailey E, Bannasch D, Binns MM, Borges AS, Brama P, da Câmara Machado A, Distl O, Felicetti M, Fox-Clipsham L, Graves KT, Guérin G, Haase B, Hasegawa T, Hemmann K, Hill EW, Leeb T, Lindgren G, Lohi H, Lopes MS, McGivney BA, Mikko S, Orr N, Penedo MC, Piercy RJ, Raekallio M, Rieder S, Røed KH, Silvestrelli M, Swinburne J, Tozaki T, Vaudin M, M Wade C, McCue ME. Genetic diversity in the modern horse illustrated from genome-wide SNP data.. PLoS One 2013;8(1):e54997.
    pubmed: 23383025doi: 10.1371/journal.pone.0054997google scholar: lookup
  2. Takashima A. Establishment of fibroblast cultures.. Curr Protoc Cell Biol 2001 May;Chapter 2:Unit 2.1.
    pubmed: 18228346doi: 10.1002/0471143030.cb0201s00google scholar: lookup
  3. Recillas-Targa F. Multiple strategies for gene transfer, expression, knockdown, and chromatin influence in mammalian cell lines and transgenic animals.. Mol Biotechnol 2006 Nov;34(3):337-54.
    pubmed: 17284781doi: 10.1385/MB:34:3:337google scholar: lookup
  4. Kong D, Nishino N, Shibusawa M, Kusano M. Establishment and characterization of human pancreatic adenocarcinoma cell line in tissue culture and the nude mouse.. Tissue Cell 2007 Aug;39(4):217-23.
    pubmed: 17560620doi: 10.1016/j.tice.2007.04.001google scholar: lookup
  5. Woods GL, White KL, Vanderwall DK, Li GP, Aston KI, Bunch TD, Meerdo LN, Pate BJ. A mule cloned from fetal cells by nuclear transfer.. Science 2003 Aug 22;301(5636):1063.
    pubmed: 12775846doi: 10.1126/science.1086743google scholar: lookup
  6. Volokhov DV, Graham LJ, Brorson KA, Chizhikov VE. Mycoplasma testing of cell substrates and biologics: Review of alternative non-microbiological techniques.. Mol Cell Probes 2011 Apr-Jun;25(2-3):69-77.
    pubmed: 21232597doi: 10.1016/j.mcp.2011.01.002google scholar: lookup
  7. Easley CA 4th, Phillips BT, McGuire MM, Barringer JM, Valli H, Hermann BP, Simerly CR, Rajkovic A, Miki T, Orwig KE, Schatten GP. Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells.. Cell Rep 2012 Sep 27;2(3):440-6.
    pubmed: 22921399doi: 10.1016/j.celrep.2012.07.015google scholar: lookup
  8. Rui R, Qiu Y, Hu Y, Fan B. Establishment of porcine transgenic embryonic germ cell lines expressing enhanced green fluorescent protein.. Theriogenology 2006 Mar 1;65(4):713-20.
    pubmed: 16026818doi: 10.1016/j.theriogenology.2005.04.033google scholar: lookup
  9. Lima-Neto JF, Fernandes CB, Alvarenga MA, Golim MA, Landim-Alvarenga FC. Viability and cell cycle analysis of equine fibroblasts cultured in vitro.. Cell Tissue Bank 2010 Aug;11(3):261-8.
    pubmed: 19404773doi: 10.1007/s10561-009-9131-6google scholar: lookup
  10. Hatami-Monazah H, Pandit RV. A cytogenetic study of the Caspian pony.. J Reprod Fertil 1979 Nov;57(2):331-3.
    pubmed: 513023doi: 10.1530/jrf.0.0570331google scholar: lookup
  11. Klingbeil MF, Herson MR, Cristo EB, dos Santos Pinto D Jr, Yoshito D, Mathor MB. Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes.. Cell Tissue Bank 2009 Aug;10(3):197-204.
    pubmed: 19184532doi: 10.1007/s10561-009-9122-7google scholar: lookup
  12. Gu Y, Li H, Miki J, Kim KH, Furusato B, Sesterhenn IA, Chu WS, McLeod DG, Srivastava S, Ewing CM, Isaacs WB, Rhim JS. Phenotypic characterization of telomerase-immortalized primary non-malignant and malignant tumor-derived human prostate epithelial cell lines.. Exp Cell Res 2006 Apr 1;312(6):831-43.
    pubmed: 16413016doi: 10.1016/j.yexcr.2005.11.029google scholar: lookup
  13. Song J, Hua S, Song K, Zhang Y. Culture, characteristics and chromosome complement of Siberian tiger fibroblasts for nuclear transfer.. In Vitro Cell Dev Biol Anim 2007 Jul-Aug;43(7):203-9.
    pubmed: 17763919doi: 10.1007/s11626-007-9043-3google scholar: lookup
  14. Richer CL, Power MM, Klunder LR, McFeely RA, Kent MG. Standard karyotype of the domestic horse (Equus caballus). Committee for standardized karyotype of Equus caballus. The Second International Conference for Standardization of Domestic Animal Karyotypes, INRA, Jouy-en Josas, France, 22nd-26th May 1989.. Hereditas 1990;112(3):289-93.
    pubmed: 1976611doi: 10.1111/j.1601-5223.1990.tb00069.xgoogle scholar: lookup
  15. Cooper JK, Sykes G, King S, Cottrill K, Ivanova NV, Hanner R, Ikonomi P. Species identification in cell culture: a two-pronged molecular approach.. In Vitro Cell Dev Biol Anim 2007 Nov-Dec;43(10):344-51.
    pubmed: 17934781doi: 10.1007/s11626-007-9060-2google scholar: lookup
  16. Escriou V, Carrière M, Bussone F, Wils P, Scherman D. Critical assessment of the nuclear import of plasmid during cationic lipid-mediated gene transfer.. J Gene Med 2001 Mar-Apr;3(2):179-87.
    pubmed: 11318117doi: 10.1002/jgm.174google scholar: lookup
  17. Amirinia C, Seyedabadi H, Banabazi MH, Kamali MA. Bottleneck study and genetic structure of Iranian Caspian horse population using microsatellites.. Pak J Biol Sci 2007 May 1;10(9):1540-3.
    pubmed: 19069972doi: 10.3923/pjbs.2007.1540.1543google scholar: lookup

Citations

This article has been cited 6 times.
  1. Elyasi Gorji Z, Farzaneh P, Nasimian A, Ganjibakhsh M, Izadpanah M, Farghadan M, Vakhshiteh F, Rahmati H, Shahzadeh Fazeli SA, Khaledi H, Daneshvar Amoli A. Cryopreservation of Iranian Markhoz goat fibroblast cells as an endangered national genetic resource. Mol Biol Rep 2021 Sep;48(9):6241-6248.
    doi: 10.1007/s11033-021-06534-3pubmed: 34398426google scholar: lookup
  2. Borges AA, Lira GPO, Nascimento LE, Santos MVO, Oliveira MF, Silva AR, Pereira AF. Isolation, characterization, and cryopreservation of collared peccary skin-derived fibroblast cell lines. PeerJ 2020;8:e9136.
    doi: 10.7717/peerj.9136pubmed: 32547858google scholar: lookup
  3. Wang T, Li Z, Zheng D, Liu W, Huang P, Zeng Z, Xu C, Wang B, Wei J. Establishment and characterization of a fibroblast cell line from postmortem skin of an adult Chinese muntjac (Muntiacus reevesi). In Vitro Cell Dev Biol Anim 2020 Feb;56(2):97-102.
    doi: 10.1007/s11626-019-00422-8pubmed: 31898011google scholar: lookup
  4. Moghanjoghi SM, Ganjibakhsh M, Gohari NS, Izadpanah M, Rahmati H, Gorji ZE, Mohebali N, Vakhshiteh F, Farzaneh P. Establishment and characterization of rough-tailed gecko original tail cells. Cytotechnology 2018 Oct;70(5):1337-1347.
    doi: 10.1007/s10616-018-0223-7pubmed: 29948549google scholar: lookup
  5. Daneshvar Amoli A, Shahzadeh Fazeli SA, Aminafshar M, Emam Jomeh Kashan N, Khaledi H. Identification of Bactrian camel cell lines using genetic markers. In Vitro Cell Dev Biol Anim 2018 Apr;54(4):265-271.
    doi: 10.1007/s11626-018-0238-6pubmed: 29497968google scholar: lookup
  6. Liao B, Cui Y, Yu S, He J, Yang X, Zou S, Li S, Zhao P, Xu H, Long M, Wang X. Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens). Front Vet Sci 2023;10:1277586.
    doi: 10.3389/fvets.2023.1277586pubmed: 38046572google scholar: lookup
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