Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids.
Abstract: It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of primary fibroblasts with BPV-1 DNA. Previously detected BPV-1 genome variations in equine sarcoids are also found in sarcoid cell lines, and only variant BPV-1 genomes can transform equine cells. These equine cell lines are morphologically transformed, proliferate faster than parental cells, have an extended life span and can grow independently of substrate. These characteristics are more marked the higher the level of viral E5, E6 and E7 gene expression. These findings confirm that the virus has an active role in the induction of sarcoids and the lines will be invaluable for further studies on the role of BPV-1 in sarcoid pathology.
Publication Date: 2008-01-11 PubMed ID: 18191170DOI: 10.1016/j.virol.2007.11.037Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research explores the creation of horse cell lines containing BPV-1 genomes and expressing relevant viral genes, which are linked to equine sarcoids. The cell lines, sourced from sarcoid biopsies or generated in vitro, offer an insight into the virus’s active role in sarcoid induction and provide essential resources for future studies on BPV-1’s effect on sarcoid pathology.
Generation of Equine Cell Lines
- The research involves the generation of horse cell lines that have BPV-1 genomes and express viral genes, either by explanting these cells from sarcoid biopsies or creating them in vitro through transfecting primary fibroblasts with BPV-1 DNA.
BPV-1 Genome Variations
- The study found that previously noticed BPV-1 genome variations in equine sarcoids were also present in sarcoid cell lines. Significantly, only variant BPV-1 genomes could transform horse cells.
Characteristics of Transformed Equine Cells
- Evolving from parental cells, the transformed equine cells undergo a morphological change and proliferate faster. They also extend their lifespan and can grow independently of the substrate.
- These features are more prominent as the viral E5, E6, and E7 gene expression level increases. Therefore, it suggests that the varying level of these gene expressions could have a significant impact on the cell’s transformation and proliferation.
Relevance of the Findings
- The study confirms the virus’s active role in inducing sarcoids, a common skin condition among horses, providing critical insights into how BPV-1 contributes to the sarcoid pathology.
- The generated cell lines provide a valuable resource for further investigations into the role of BPV-1 in sarcoid pathology, paving the way for potential advancements in the treatment or prevention of this equine disease.
Cite This Article
APA
Yuan ZQ, Gault EA, Gobeil P, Nixon C, Campo MS, Nasir L.
(2008).
Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids.
Virology, 373(2), 352-361.
https://doi.org/10.1016/j.virol.2007.11.037 Publication
Researcher Affiliations
- Institute of Comparative Medicine, University of Glasgow Faculty of Veterinary Medicine, Bearsden Road, Glasgow G61 1QH, UK.
MeSH Terms
- Animals
- Bovine papillomavirus 1 / genetics
- Bovine papillomavirus 1 / pathogenicity
- Cell Line, Transformed
- Cell Proliferation
- Cell Transformation, Viral
- Gene Expression
- Genome, Viral
- Horse Diseases / virology
- Horses
- Models, Biological
- Oncogenes
- Papillomavirus Infections / veterinary
- Papillomavirus Infections / virology
- Skin Neoplasms / veterinary
- Skin Neoplasms / virology
- Tumor Suppressor Protein p53 / antagonists & inhibitors
- Tumor Suppressor Protein p53 / metabolism
Citations
This article has been cited 18 times.- Jindra C, Hainisch EK, Brandt S. Immunotherapy of Equine Sarcoids-From Early Approaches to Innovative Vaccines.. Vaccines (Basel) 2023 Mar 30;11(4).
- Hainisch EK, Jindra C, Reicher P, Miglinci L, Brodesser DM, Brandt S. Bovine Papillomavirus Type 1 or 2 Virion-Infected Primary Fibroblasts Constitute a Near-Natural Equine Sarcoid Model.. Viruses 2022 Nov 28;14(12).
- Podstawski P, Ropka-Molik K, Semik-Gurgul E, Samiec M, Skrzyszowska M, Podstawski Z, Szmatoła T, Witkowski M, Pawlina-Tyszko K. Tracking the Molecular Scenarios for Tumorigenic Remodeling of Extracellular Matrix Based on Gene Expression Profiling in Equine Skin Neoplasia Models.. Int J Mol Sci 2022 Jun 10;23(12).
- Podstawski P, Ropka-Molik K, Semik-Gurgul E, Samiec M, Skrzyszowska M, Podstawski Z, Szmatoła T, Witkowski M, Pawlina-Tyszko K. Assessment of BPV-1 Mediated Matrix Metalloproteinase Genes Deregulation in the In Vivo and In Vitro Models Designed to Explore Molecular Nature of Equine Sarcoids.. Cells 2022 Apr 8;11(8).
- Podstawski P, Samiec M, Skrzyszowska M, Szmatoła T, Semik-Gurgul E, Ropka-Molik K. The Induced Expression of BPV E4 Gene in Equine Adult Dermal Fibroblast Cells as a Potential Model of Skin Sarcoid-like Neoplasia.. Int J Mol Sci 2022 Feb 10;23(4).
- Jindra C, Hainisch EK, Rümmele A, Wolschek M, Muster T, Brandt S. Influenza virus vector iNS1 expressing bovine papillomavirus 1 (BPV1) antigens efficiently induces tumour regression in equine sarcoid patients.. PLoS One 2021;16(11):e0260155.
- Podstawski P, Witarski W, Szmatoła T, Bugno-Poniewierska M, Ropka-Molik K. Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid.. Animals (Basel) 2020 May 19;10(5).
- Cortés-Araya Y, Amilon K, Rink BE, Black G, Lisowski Z, Donadeu FX, Esteves CL. Comparison of Antibacterial and Immunological Properties of Mesenchymal Stem/Stromal Cells from Equine Bone Marrow, Endometrium, and Adipose Tissue.. Stem Cells Dev 2018 Nov 1;27(21):1518-1525.
- Martano M, Corteggio A, Restucci B, De Biase ME, Borzacchiello G, Maiolino P. Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study.. BMC Vet Res 2016 Feb 2;12:24.
- Mosseri S, Hetzel U, Hahn S, Michaloupoulou E, Sallabank HC, Knottenbelt DC, Kipar A. Equine sarcoid: In situ demonstration of matrix metalloproteinase expression.. Vet J 2014 Nov;202(2):279-85.
- Trewby H, Ayele G, Borzacchiello G, Brandt S, Campo MS, Del Fava C, Marais J, Leonardi L, Vanselow B, Biek R, Nasir L. Analysis of the long control region of bovine papillomavirus type 1 associated with sarcoids in equine hosts indicates multiple cross-species transmission events and phylogeographical structure.. J Gen Virol 2014 Dec;95(Pt 12):2748-2756.
- Rich T, Henderson LB, Becker DL, Cornell H, Patterson-Kane JC. Indicators of replicative damage in equine tendon fibroblast monolayers.. BMC Vet Res 2013 Sep 11;9:180.
- Altamura G, Corteggio A, Nasir L, Yuan ZQ, Roperto F, Borzacchiello G. Analysis of activated platelet-derived growth factor β receptor and Ras-MAP kinase pathway in equine sarcoid fibroblasts.. Biomed Res Int 2013;2013:283985.
- Cotugno R, Gallotta D, d'Avenia M, Corteggio A, Altamura G, Roperto F, Belisario MA, Borzacchiello G. BAG3 protects bovine papillomavirus type 1-transformed equine fibroblasts against pro-death signals.. Vet Res 2013 Jul 22;44(1):61.
- Finlay M, Yuan Z, Morgan IM, Campo MS, Nasir L. Equine sarcoids: Bovine Papillomavirus type 1 transformed fibroblasts are sensitive to cisplatin and UVB induced apoptosis and show aberrant expression of p53.. Vet Res 2012 Dec 4;43(1):81.
- Altamura G, Strazzullo M, Corteggio A, Francioso R, Roperto F, D'Esposito M, Borzacchiello G. O(6)-methylguanine-DNA methyltransferase in equine sarcoids: molecular and epigenetic analysis.. BMC Vet Res 2012 Nov 10;8:218.
- Strazzullo M, Corteggio A, Altamura G, Francioso R, Roperto F, D'Esposito M, Borzacchiello G. Molecular and epigenetic analysis of the fragile histidine triad tumour suppressor gene in equine sarcoids.. BMC Vet Res 2012 Mar 16;8:30.
- Yuan ZQ, Nicolson L, Marchetti B, Gault EA, Campo MS, Nasir L. Transcriptional changes induced by bovine papillomavirus type 1 in equine fibroblasts.. J Virol 2008 Jul;82(13):6481-91.
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