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Virology2008; 373(2); 352-361; doi: 10.1016/j.virol.2007.11.037

Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids.

Abstract: It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of primary fibroblasts with BPV-1 DNA. Previously detected BPV-1 genome variations in equine sarcoids are also found in sarcoid cell lines, and only variant BPV-1 genomes can transform equine cells. These equine cell lines are morphologically transformed, proliferate faster than parental cells, have an extended life span and can grow independently of substrate. These characteristics are more marked the higher the level of viral E5, E6 and E7 gene expression. These findings confirm that the virus has an active role in the induction of sarcoids and the lines will be invaluable for further studies on the role of BPV-1 in sarcoid pathology.
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Publication Date: 2008-01-11 PubMed ID: 18191170DOI: 10.1016/j.virol.2007.11.037Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the creation of horse cell lines containing BPV-1 genomes and expressing relevant viral genes, which are linked to equine sarcoids. The cell lines, sourced from sarcoid biopsies or generated in vitro, offer an insight into the virus’s active role in sarcoid induction and provide essential resources for future studies on BPV-1’s effect on sarcoid pathology.

Generation of Equine Cell Lines

  • The research involves the generation of horse cell lines that have BPV-1 genomes and express viral genes, either by explanting these cells from sarcoid biopsies or creating them in vitro through transfecting primary fibroblasts with BPV-1 DNA.

BPV-1 Genome Variations

  • The study found that previously noticed BPV-1 genome variations in equine sarcoids were also present in sarcoid cell lines. Significantly, only variant BPV-1 genomes could transform horse cells.

Characteristics of Transformed Equine Cells

  • Evolving from parental cells, the transformed equine cells undergo a morphological change and proliferate faster. They also extend their lifespan and can grow independently of the substrate.
  • These features are more prominent as the viral E5, E6, and E7 gene expression level increases. Therefore, it suggests that the varying level of these gene expressions could have a significant impact on the cell’s transformation and proliferation.

Relevance of the Findings

  • The study confirms the virus’s active role in inducing sarcoids, a common skin condition among horses, providing critical insights into how BPV-1 contributes to the sarcoid pathology.
  • The generated cell lines provide a valuable resource for further investigations into the role of BPV-1 in sarcoid pathology, paving the way for potential advancements in the treatment or prevention of this equine disease.

Cite This Article

APA
Yuan ZQ, Gault EA, Gobeil P, Nixon C, Campo MS, Nasir L. (2008). Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids. Virology, 373(2), 352-361. https://doi.org/10.1016/j.virol.2007.11.037

Publication

Virology
ISSN: 0042-6822
NlmUniqueID: 0110674
Country: United States
Language: English
Volume: 373
Issue: 2
Pages: 352-361

Researcher Affiliations

Yuan, Z Q
  • Institute of Comparative Medicine, University of Glasgow Faculty of Veterinary Medicine, Bearsden Road, Glasgow G61 1QH, UK.
Gault, E A
    Gobeil, P
      Nixon, C
        Campo, M S
          Nasir, L

            MeSH Terms

            • Animals
            • Bovine papillomavirus 1 / genetics
            • Bovine papillomavirus 1 / pathogenicity
            • Cell Line, Transformed
            • Cell Proliferation
            • Cell Transformation, Viral
            • Gene Expression
            • Genome, Viral
            • Horse Diseases / virology
            • Horses
            • Models, Biological
            • Oncogenes
            • Papillomavirus Infections / veterinary
            • Papillomavirus Infections / virology
            • Skin Neoplasms / veterinary
            • Skin Neoplasms / virology
            • Tumor Suppressor Protein p53 / antagonists & inhibitors
            • Tumor Suppressor Protein p53 / metabolism

            Citations

            This article has been cited 21 times.
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