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Home / Equine Research Bank / Iran J Vet Res / Evaluating the efficacy of surgical excision and topical dim...
Iranian journal of veterinary research2021; 21(4); 301-307;

Evaluating the efficacy of surgical excision and topical dimethyl sulphoxide (DMSO) in the treatment of equine cutaneous pythiosis.

Abstract: Cutaneous pythiosis in horses is a chronic ulcerative granulomatous disease caused by the oomycete Pythium insidiosum. Objective: The objective of the present study was to evaluate the response of cutaneous pythiosis in horses to surgical excision and topical dimethyl sulphoxide (DMSO). Methods: Thirty horses were presented clinically with pruritus, fistulae discharging serosanguineous fluid, and output kunkers in different body areas (limb, abdomen, neck, and face). The clinical diagnosis was confirmed by isolation of the causative agent and histopathology. All animals were treated by surgical excision alone, or surgical excision followed by topical DMSO. The healing process was monitored every week macroscopically to evaluate the response to treatment until complete recovery. Results: The existence of Pythium insidiosum was confirmed in all cases. Histologically, affected horses were characterized by granulation tissue with abundant eosinophils. The size of wounds and the clinical features of pythiosis lesions decreased more after surgical debridement with DMSO application than surgical excision alone. The cutaneous pythiosis lesions were completely recovered at 35 ± 7 and 60 ± 5 days after the surgical excision with topical DMSO and surgical excision alone, respectively. Conclusions: The combination of surgical excision and topical DMSO is found an effective treatment for cutaneous pythiosis in horses.
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Publication Date: 2021-02-16 PubMed ID: 33584843PubMed Central: PMC7871735
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the effect of a combination of surgical removal and topical dimethyl sulphoxide (DMSO) treatment on cutaneous pythiosis, a skin disease in horses. The study concluded that using surgical excision with DMSO enabled a quicker healing process compared to surgery alone.

Objectives and Methods

  • The study wanted to evaluate how effective surgical removal combined with topical DMSO was in handling cutaneous pythiosis in horses. Pythiosis is a serious skin disease in horses characterized by chronic ulcers and granulomas.
  • Thirty horses with clinical signs of pythiosis — such as itching, fluid-discharging fistulas, and output kunkers in various body areas — were selected for the study.
  • The diagnosis of pythiosis was confirmed by singling out the causative agent and carrying out histopathology.
  • The horses were treated either by surgical removal alone or coupled with topical DMSO. The recovery process was monitored weekly to gauge the response to treatment until there was a complete recovery.

Results

  • Confirmation of pythiosis was seen in all cases. Observing the affected horses histologically revealed granulation tissue with an abundance of eosinophils.
  • There was a significant decrease in the size of wounds and clinical characteristics of pythiosis lesions after surgical debridement combined with DMSO application compared to surgery alone.
  • The lesions from cutaneous pythiosis were fully healed after about 35 days with surgery and DMSO, and around 60 days with surgery alone.

Conclusions

  • The research concludes that using surgical removal combined with topical DMSO treatment is effective for managing cutaneous pythiosis in horses.
  • This combination treatment notably speeds up the healing process compared to utilizing surgical excision alone.

Cite This Article

APA
Atiba A, Ghazy A, Hamad MH. (2021). Evaluating the efficacy of surgical excision and topical dimethyl sulphoxide (DMSO) in the treatment of equine cutaneous pythiosis. Iran J Vet Res, 21(4), 301-307.

Publication

Iranian journal of veterinary research
ISSN: 1728-1997
NlmUniqueID: 101660030
Country: Iran
Language: English
Volume: 21
Issue: 4
Pages: 301-307

Researcher Affiliations

Atiba, A
  • Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt.
Ghazy, A
  • Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt.
Hamad, M H
  • Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.

References

This article includes 28 references
  1. Atiba A, Ghazy A. The effects of topical dimethyle sulfoxide on second-degree burn wound healing in dogs.. A. J. Vet. Sci. 2015;45:6–12.
  2. Cafarchia C, Figueredo LA, Otranto D. Fungal diseases of horses.. Vet. Microbiol. 2013;167:215–234.
    pubmed: 23428378
  3. Capriotti K, Capriotti JA. Dimethyl sulfoxide: History, chemistry, and clinical utility in dermatology.. J. Clin. Aesthet. Dermatol. 2012;5:24–26.
    pmc: PMC3460663pubmed: 23050031
  4. Cardona-Alvarez J, Vargas-Vilória M, Patarroyo-Salcedo J. Pythiosis cutaneous in horses treated with triamcinolone acetonide Part 2 Histological and histochemical description.. Rev. MVZ. Cordoba. 2017;22:5638–5652.
  5. Carvalho ADM, Munhoz TCP, Toma HS, dos Santos JPV, de Oliveira LGS, Boabaid FM, Xavier AB, Kommers GD. The use of fluconazole associated with surgical excision in the treatment of equine cutaneous pythiosis.. Semin. Agrar. 2019;40:3079–3088.
  6. Chaffin MK, Schumacher J, McMullan WC. Cutaneous pythiosis in the horse.. Vet. Clin. North Am. Equine Pract. 1995;11:91–103.
    pubmed: 7634168
  7. Chitasombat MN, Jongkhajornpong P, Lekhanont K, Krajaejun T. Recent update in diagnosis and treatment of human pythiosis.. Peer J. 2020;8:1–30.
    pmc: PMC7036273pubmed: 32117626
  8. Dias DPM, Doria RGS, Pereira RN, Canola PRA, Di Filippo PA. Topical treatment using Amphotericin B and DMSO for an atypically located equine cutaneous pythiosis.. Acta Sci. Vet. 2012;40:75–78.
  9. Doria RGS, Carvalho MB, Freitas SH, Laskoski LM, Colodel EM, Mendonca FS, Silva MAG, Grigoletto R, Neto PF. Evaluation of intravenous regional perfusion with amphotericin B and dimethylsulfoxide to treat horses for pythiosis of a limb.. BMC Vet. Res. 2015;11:1–7.
    pmc: PMC4502520pubmed: 26174778
  10. Doria RGS, Freitas SH, Linardi RL, Mendonca FD, Arruda LP, Boabaid FM, Valadao CA. Treatment of pythiosis in equine limbs using intravenous regional perfusion of Amphotericin B.. Vet. Surg. 2012;41:759–765.
    pubmed: 22846053
  11. Duimel-Peeters IGP, Houwing RH, Teunissen CP, Berger MPF, Snoeckx LHEH, Halfens RJG. A systematic review of the efficacy of topical skin application of dimethyl sulfoxide on wound healing and as an anti-inflammatory drug.. Wounds. 2003;15:361–370.
  12. Elkhenany H, Nabil S, Abu-Ahmed H, Mahmoud H, Korritum A, Khalifa H. Treatment and outcome of horses with cutaneous pythiosis, and meta-analysis of similar reports.. Slov. Vet. Res. 2019;56:281–291.
  13. Gaastra W, Lipman LJA, De Cock AWAM, Exel TK, Pegge RBG, Scheuwater J, Vilela R, Mendoza L. Pythium insidiosum: An overview.. Vet. Microbiol. 2010;146:1–16.
    pubmed: 20800978
  14. Grooters AM. Pythiosis and zygomycosis.. In: Sellon DC, editor. Equine infectious diseases. St Louis, Saunders Elsevier: 2013. pp. 415–421.
  15. Hassan AS. The antibacterial activity of dimethyl sulfoxide (dmso) with and without of some ligand complexes of the transitional metal ions of ethyl coumarin against bacteria isolate from burn and wound infection.. J. Nat. Sci. Res. 2014;4:106–111.
  16. Leal ABM, Leal AT, Santurio JM, Kommers GD, Catto JB. Equine pythiosis in the Brazilian Pantanal region: Clinical and pathological findings of typical and atypical cases.. Pesqui. Vet. Bras. 2001;21:151–156.
  17. Lerksuthirat T, Sangcakul A, Lohnoo T, Yingyong W, Rujirawat T, Krajaejun T. Evolution of the sterol biosynthetic pathway of Pythium insidiosum and related oomycetes contributes to antifungal drug resistance.. Antimicrob. Agents Chemother. 2017;61:1–14.
    pmc: PMC5365696pubmed: 28115356
  18. Loreto ES, Alves SH, Santurio JM, Nogueira CW, Zeni G. Diphenyl diselenide in vitro and in vivo activity against the oomycete Pythium insidiosum.. Vet. Microbiol. 2012;156:222–226.
    pubmed: 22055205
  19. Loreto ES, Tondolo JS, Pilotto MB, Alves SH, Santurio JM. New insights into the in vitro susceptibility of Pythium insidiosum.. Antimicrob. Agents Chemother. 2014;58:7534–7537.
    pmc: PMC4249561pubmed: 25223997
  20. Mendoza L, Mandy W, Glass R. An improved Pythium insidiosum-vaccine formulation with enhanced immunotherapeutic properties in horses and dogs with pythiosis.. Vaccine. 2003;21:2797–2804.
    pubmed: 12798620
  21. Mosbah E, Karrouf GIA, Younis EA, Saad HS, Ahdy A, Zaghloul AE. Diagnosis and surgical management of pythiosis in draft horses: report of 33 cases in Egypt.. J. Equine Vet. Sci. 2012;32:164–169.
  22. Randhawa MA. The effect of dimethyl sulfoxide (DMSO) on the growth of dermatophytes.. Japanese J. Med. Mycol. 2006;47:313–318.
    pubmed: 17086165
  23. Randhawa MA. Dimethyl sulfoxide (DMSO) inhibits the germination of Candida albicans and the arthrospores of Trichophyton mentagrophytes.. Japanese J. Med. Mycol. 2008;49:125–128.
    pubmed: 18451594
  24. Randhawa MA, Hamad S, Aljabre M. Dimethyl sulfoxide (DMSO) has an additive effect and alters minimal inhibitory concentrations of antifungal drugs.. J. Rawalpindi. Med. Coll. 2007;11:54–60.
  25. Rivierre C, Laprie C, Guiard-Marigny O, Bergeaud P, Berthelemy M, Guillot J. Pythiosis in Africa.. Emerg. Infect. Dis. 2005;11:479–481.
    pmc: PMC3298269pubmed: 15757572
  26. Romero A, Garcia J, Balestie S, Malfatto F, Vicentino A, Sallis ESV, Schild AL, Dutra FQ. Equine pythiosis in the Eastern wetlands of Uruguay1.. Pesqui. Vet. Bras. 2019;39:469–475.
  27. Santos CEP, Ubiali DG, Pescador CA, Zanette RA, Santurio JM, Marques LC. Epidemiological survey of equine pythiosis in the Brazilian pantanal and nearby areas: Results of 76 cases.. J. Equine Vet. Sci. 2014;34:270–274.
  28. Tarrand JJ, LaSala PR, Han XY, Rolston KV, Kontoyiannis DP. Dimethyl sulfoxide enhances effectiveness of skin antiseptics and reduces contamination rates of blood cultures.. J. Clin. Microbiol. 2012;50:1552–1557.
    pmc: PMC3347107pubmed: 22378911

Citations

This article has been cited 3 times.
  1. Pereira DIB, Botton SA, Ianiski LB, Braga CQ, Maciel AF, Melo LG, Zambrano CG, Bruhn FRP, Santurio JM. Equidae pythiosis in Brazil and the world: a systematic review of the last 63 years (1960-2023). Braz J Microbiol 2024 Sep;55(3):2969-2981.
    doi: 10.1007/s42770-024-01435-6pubmed: 38967702google scholar: lookup
  2. Yolanda H, Lohnoo T, Rujirawat T, Yingyong W, Kumsang Y, Sae-Chew P, Payattikul P, Krajaejun T. Selection of an Appropriate In Vitro Susceptibility Test for Assessing Anti-Pythium insidiosum Activity of Potassium Iodide, Triamcinolone Acetonide, Dimethyl Sulfoxide, and Ethanol. J Fungi (Basel) 2022 Oct 24;8(11).
    doi: 10.3390/jof8111116pubmed: 36354883google scholar: lookup
  3. Yolanda H, Krajaejun T. History and Perspective of Immunotherapy for Pythiosis. Vaccines (Basel) 2021 Sep 26;9(10).
    doi: 10.3390/vaccines9101080pubmed: 34696188google scholar: lookup
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