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Journal of veterinary internal medicine2017; 31(2); 568-574; doi: 10.1111/jvim.14657

Evaluation of a Chemiluminescent Immunoassay for Measurement of Equine Insulin.

Abstract: Many diagnostic tests for insulin dysregulation use reference intervals established with an insulin radioimmunoassay (RIA) that is no longer available. A chemiluminescent immunoassay (CLIA) is commonly used for the measurement of serum insulin concentration in clinical practice but requires further validation, especially at clinically relevant reference intervals. Objective: To evaluate the CLIA for measurement of equine insulin and compare it to the previously validated, but now unavailable RIA. Methods: Equine serum samples (n = 78) from clinical and experimental studies. Methods: In this experimental study, performance of the CLIA was evaluated using standard variables, including comparison with the RIA. Continuous and binary outcomes were analyzed. Results: The CLIA showed good intra-assay (coefficient of variation [CV], 1.8-2.4%) and interassay (CV, 3-7.1%) precision. Acceptable recovery on dilution (100 ± 10%) was achieved only at dilutions <1:1. Recovery on addition was acceptable. Comparison of the CLIA and RIA showed strong positive correlation (r = 0.91-0.98), with fixed and proportional bias. At 3 diagnostic cutoffs, sensitivity of CLIA compared with RIA ranged from 67 to 100% and specificity from 96 to 100%. Conclusions: The CLIA is a highly repeatable assay which is suitable for within- and between-horse comparisons. Dilution of high concentration samples should be performed with charcoal-stripped serum (CSS) and at the lowest dilution factor possible. At concentrations commonly used for diagnosis of insulin dysregulation (≤100 μIU/mL), results from the CLIA tend to be lower than from the RIA and should be interpreted accordingly. Further standardization of equine insulin assays is required.
Copyright © 2017 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2017-01-26 PubMed ID: 28124389PubMed Central: PMC5354037DOI: 10.1111/jvim.14657Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article focuses on evaluating the effectiveness of a Chemiluminescent Immunoassay (CLIA) in measuring equine insulin levels as it is compared to a previously validated but now unavailable technique known as Insulin Radioimmunoassay (RIA). The study finds that while the CLIA yields reliable results, further standardization of equine insulin assays is required.

Methodology

  • Researchers conducted an experimental study using equine serum samples obtained from previous clinical and experimental trials. The total number of samples analyzed remained 78.
  • They evaluated the performance of the CLIA in measuring equine insulin using several standard variables and then compared these results to those generated by RIA.

Results

  • The Chemiluminescent Immunoassay technique exhibited strong intra-assay and interassay accuracy, with precision defined in terms of coefficient variation (CV). These precision levels ranged between 1.8 and 2.4% for intra-assay and between 3 and 7.1% for interassay, indicating a high level of repeatability.
  • This technique also achieved an acceptable recovery rate on dilution but only at dilutions below 1:1. The recovery on direct addition was also deemed to be acceptable.
  • Strong positive correlation was observed between the results of the CLIA and RIA (r = 0.91-0.98). However, this comparison also revealed fixed and proportional biases.
  • The sensitivity of the CLIA compared to the RIA fluctuated between 67 to 100%, and specificity ranged from 96 to 100% across three diagnostic cut-offs.

Conclusions

  • From the results obtained, the study concluded that the CLIA method, due to its high repeatability, suitability for within-horse and between-horse comparisons and reasonable sensitivity, proved to be a viable technique for measuring equine insulin at clinically relevant levels.
  • However, diluting samples with high concentrations should be achieved using a charcoal-stripped serum (CSS) and at the lowest possible dilution factor.
  • It should be noted that at lower insulin concentrations, the result from the CLIA is lower than from the RIA, indicating some degree of underestimation. This suggests the need for adjusting the interpretation of results accordingly.
  • The researchers ultimately recommended further standardization of equine insulin assays in order to attain more accurate and consistent results in clinical diagnosis.

Cite This Article

APA
Carslake HB, Pinchbeck GL, McGowan CM. (2017). Evaluation of a Chemiluminescent Immunoassay for Measurement of Equine Insulin. J Vet Intern Med, 31(2), 568-574. https://doi.org/10.1111/jvim.14657

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 31
Issue: 2
Pages: 568-574

Researcher Affiliations

Carslake, H B
  • Institute of Aging and Chronic Disease, Faculty of Health and Life Sciences, University of Liverpool, Neston, Wirral, UK.
Pinchbeck, G L
  • Institute of Infection and Global Health, Faculty of Health and Life Sciences, University of Liverpool, Neston, Wirral, UK.
McGowan, C M
  • Institute of Aging and Chronic Disease, Faculty of Health and Life Sciences, University of Liverpool, Neston, Wirral, UK.

MeSH Terms

  • Animals
  • Horses / blood
  • Immunoassay / veterinary
  • Insulin / blood
  • Luminescent Measurements / methods
  • Luminescent Measurements / veterinary
  • Radioimmunoassay / methods
  • Radioimmunoassay / veterinary
  • Reproducibility of Results
  • Sensitivity and Specificity

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Citations

This article has been cited 20 times.
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