Evaluation of a single injection of 99mTc-labeled diethylenetriaminepentaacetic acid for measuring glomerular filtration rate in horses.
Abstract: Glomerular filtration rate (GFR) was measured in 12 clinically normal horses, using the standard inulin clearance method, and values were compared with values for 2 methods, using a single rapid IV injection of 99mTc-labeled diethylenetriaminepentaacetic acid (99mTc-DTPA). The first 99mTc-DTPA method used a 2-compartment model to calculate GFR blood clearance of the tracer. The second method used sequential digital gamma camera images of the kidneys to determine fractional accumulation of the total dose of the tracer in the kidneys (percentage of injected dose, gamma camera) from 0 to 10 minutes after radionuclide administration. Linear correlation among the 3 methods was determined. Mean (+/- SD) GFR, using the inulin clearance method, was 154.67 +/- 42.28 ml/min/100 kg of body weight. Mean GFR, using the 2-compartment blood clearance curve, was 146.92 +/- 27.49 ml/min/100 kg. Mean GFR, using percentage of injected dose (gamma camera method) was 154.7 +/- 22.00 ml/min/100 kg. The percentage of injected dose (gamma camera method) did not correlate significantly to the inulin clearance results. However, a significant (r = 0.666, P less than 0.018) correlation was observed between the inulin method and the 99mTc-DTPA blood clearance method. Significant (P less than 0.0001) difference also was observed in the split function of the equine kidneys, with GFR of the right kidney contributing 60.1 +/- 9.12% of the total function, as determined by 99mTc-DTPA gamma camera imaging. Because the 99mTc-DTPA blood clearance method does not require urine collection, it may be a more practical procedure to measure GFR in the horse.
Publication Date: 1992-05-01 PubMed ID: 1524306
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study sought to evaluate a simpler method for measuring the Glomerular filtration rate (GFR) in horses. The researchers compared the GFR measurements obtained through traditional inulin clearance method with newer methods involving a single injection of a radioactive tracer (99mTc-DTPA) and found significant correlation between the inulin method and 99mTc-DTPA blood clearance.
Research Methodology
- The study involved 12 horses that were clinically normal. The GFR of these horses was measured using three methods, including the standard inulin clearance method and two methods involving a single injection of 99mTc-labeled diethylenetriaminepentaacetic acid (99mTc-DTPA).
- The first 99mTc-DTPA method used a two-compartment model to compute the GFR by observing the clearance of the tracer from the blood.
- The second 99mTc-DTPA method used digital gamma camera images of the kidneys to determine the buildup of the tracer in the kidneys, taken 0 to 10 minutes after the radionuclide was administered.
Research Findings
- The average GFR value obtained through the inulin clearance method was approximately 154.67 ml/min/100 kg of body weight.
- For the two-compartment 99mTc-DTPA method, the average GFR recorded was approximately 146.92 ml/min/100 kg.
- The GFR value obtained through the gamma camera method was approximately 154.7 ml/min/100 kg.
- The observations from the gamma camera method did not significantly align with the results from the inulin clearance method.
- However, there was a significant correlation (r=0.666, P less than 0.018) seen between the outcomes generated from the 99mTc-DTPA blood clearance method and the standard inulin clearance method.
- Through the 99mTc-DTPA gamma camera imaging, a significant difference was observed in the split function of the horse’s kidneys, with a greater GFR contribution from the right kidney.
Implications & Conclusion
- Considering the correlation observed between the inulin clearance method and the 99mTc-DTPA blood clearance method, it can be deduced that the latter might be a more practical choice for measuring GFR in horses.
- The added advantage of the 99mTc-DTPA blood clearance method is that it is less invasive as it doesn’t require urine collection.
Cite This Article
APA
Walsh DM, Royal HD.
(1992).
Evaluation of a single injection of 99mTc-labeled diethylenetriaminepentaacetic acid for measuring glomerular filtration rate in horses.
Am J Vet Res, 53(5), 776-780.
Publication
Researcher Affiliations
- Animal Health Foundation, St Louis, MO 63011.
MeSH Terms
- Animals
- Female
- Glomerular Filtration Rate / veterinary
- Horses / physiology
- Inulin
- Kidney / diagnostic imaging
- Kidney / physiology
- Radionuclide Imaging
- Technetium Tc 99m Pentetate
Citations
This article has been cited 3 times.- Jeong YS, Jusko WJ. Meta-Assessment of Metformin Absorption and Disposition Pharmacokinetics in Nine Species.. Pharmaceuticals (Basel) 2021 Jun 7;14(6).
- Freccero F, Petrucelli M, Cipone M, Nocera I, Sgorbini M. Doppler evaluation of renal resistivity index in healthy conscious horses and donkeys.. PLoS One 2020;15(2):e0228741.
- Satoh H, Abe S, Kato M, Saito J, Furuhama K. Optimum conditions for serum clearance of iodixanol, applicable to the estimation of glomerular filtration rate in horses.. Vet Res Commun 2011 Oct;35(7):463-8.
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