FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / BMC Vet Res / Evaluation of allogeneic freeze-dried platelet lysate in car...
BMC veterinary research2019; 15(1); 386; doi: 10.1186/s12917-019-2118-z

Evaluation of allogeneic freeze-dried platelet lysate in cartilage exposed to interleukin 1-β in vitro.

Abstract: Platelet-rich plasma (PRP) as well as other platelet-derived products have been used as a potential disease-modifying treatment for musculoskeletal diseases, such as osteoarthritis (OA). The restorative properties of such products rely mainly on the high concentrations of growth factors, demonstrating encouraging results experimentally and clinically. Yet, the autologous blood-derived nature of the PRP product lead to limitations that precludes it's widespread use. The main limitations for PRP use are; product variability, the need for minimum laboratory settings in most cases, and the need for storage at low temperatures to preserve its properties. Based on these limitations, the objective of this study was to investigate an allogeneic off-the-shelf platelet lysate (PL) in cartilage exposed to interleukin 1β (IL-1β). For this purpose, blood and cartilage were harvested from eight skeletally mature and healthy horses. Blood was processed into PL aliquots and divided into three groups (Frozen, Freeze-dried and Filtered freeze-dried), used in autologous and allogeneic conditions and in three different concentrations (1.5, 3 and 6-fold). Different PL preparations were then applied in cartilage culture with interleukin-1 beta and cultured for 10 days. Cartilage and media samples were collected and analyzed for total GAG and 35SO4-labeled GAG content. Results: No significant differences between the controls and PL groups in cartilage and media were demonstrated. The effects of PL on cartilage matrix were concentration dependent and intermediate concentrations (3-fold) in PL showed increased 35SO4-labelled GAG in cartilage. Conclusions: In conclusion, the allogeneic freeze-dried PL presented equivalent effects compared to frozen autologous PL. Intermediate platelet concentration on average demonstrated improved results, demonstrating less GAG loss compared to other concentrations.
Get Full Text
Publication Date: 2019-11-01 PubMed ID: 31675958PubMed Central: PMC6824121DOI: 10.1186/s12917-019-2118-zGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aims to investigate the effects of allogeneic freeze-dried platelet lysate (PL) on cartilage exposed to interleukin 1beta. It finds that allogeneic freeze-dried PL exhibits equivalent results to frozen autologous PL, with intermediate platelet concentrations showing improved outcomes.

Methodology and Experimentation Procedure

  • The researchers obtained blood and cartilage from eight mature, healthy horses. They processed the blood into PL aliquots, which they divided into three groups: Frozen, Freeze-dried, and Filtered freeze-dried. These groups were used in autologous and allogeneic conditions and in three different concentration levels – 1.5, 3, and 6-fold.
  • The cartilage was cultured with interleukin-1 beta and exposed to the different PL preparations for ten days. Samples from the cartilage and media were collected for analysis once the incubation period was over.

Glycosaminoglycans (GAG) Measurement

  • The researchers measured Glycosaminoglycans (GAG), a type of molecule found in cartilage, to assess the impact of the PL on the cartilage. They specifically looked out for total GAG and sulphate (SO)-labelled GAG levels in their testing.

Results and Findings

  • According to their findings, no significant differences were present between the controls and PL groups in the cartilage and media. This implies that the PL did not have an adverse or substantial effect on the cartilage.
  • The researchers, however, noticed that the impact of PL on the cartilage matrix was concentration-dependent. This was evident in their observation that using an intermediate concentration (3-fold) in the PL led to an increase in SO-labelled GAG in the cartilage.
  • Notably, the allogeneic freeze-dried PL had effects comparable to those of frozen autologous PL. Intermediate platelet concentrations displayed superior outcomes, showing less GAG loss compared to higher and lower concentrations.

Research Implications and Conclusion

  • The findings of this study have significant implications for treatment methods for osteoarthritis and other musculoskeletal diseases. The comparable effects of allogeneic freeze-dried load platelet lysate may present a more feasible and effective alternative to traditional blood-derived products like PRP (Platelet-Rich Plasma), overcoming their limitations such as variability, need for minimal laboratory settings, and requirement for low-temperature storage.
  • However, there’s a need for additional research to establish the optimal concentration levels to maximize the potential therapeutic effect. Such studies might help provide a clearer understanding of the optimal dosage for maximum efficacy.

Cite This Article

APA
Camargo Garbin L, McIlwraith CW, Frisbie DD. (2019). Evaluation of allogeneic freeze-dried platelet lysate in cartilage exposed to interleukin 1-β in vitro. BMC Vet Res, 15(1), 386. https://doi.org/10.1186/s12917-019-2118-z

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 15
Issue: 1
Pages: 386

Researcher Affiliations

Camargo Garbin, Livia
  • Department of Clinical Veterinary Sciences, School of Veterinary Medicine, Faculty of Medical Sciences, University of West Indies, St. Augustine Campus, St. Augustine, Trinidad and Tobago. Livia.Garbin@sta.uwi.edu.
McIlwraith, C Wayne
  • C.Wayne McIlwraith Translational Medicine Institute, Orthopaedic Research Center, Colorado State University, 2350 Gillette Drive, Fort Collins, CO, 80523, USA.
Frisbie, David D
  • C.Wayne McIlwraith Translational Medicine Institute, Orthopaedic Research Center, Colorado State University, 2350 Gillette Drive, Fort Collins, CO, 80523, USA.

MeSH Terms

  • Animals
  • Blood Platelets / chemistry
  • Cartilage / drug effects
  • Freeze Drying
  • Glycosaminoglycans / metabolism
  • Horses
  • Interleukin-1beta / pharmacology
  • Platelet-Rich Plasma
  • Tissue Culture Techniques / veterinary

Grant Funding

  • 238770/2012-5 / Conselho Nacional de Desenvolvimento Científico e Tecnológico

Conflict of Interest Statement

The authors declare that they have no competing interests.

References

This article includes 35 references
  1. Hayami T. Osteoarthritis of the knee joint as a cause of musculoskeletal ambulation disability symptom complex (MADS). Clin Calcium 2008.
    pubmed: 18974445doi: clica081115741580google scholar: lookup
  2. Abrams GD, Frank RM, Fortier LA, Cole BJ. Platelet-rich plasma for articular cartilage repair. Sports Med Arthrosc 2013;21:213–219.
    pubmed: 24212369
  3. Anitua E, Sánchez M, Nurden AT. Platelet-released growth factors enhance the secretion of hyaluronic acid and induce hepatocyte growth factor production by synovial fibroblasts from arthritic patients. Rheumatology (Oxford) 2007;46:1769–1772.
    pubmed: 17942474
  4. Kon E, Mandelbaum B, Buda R. Platelet-rich plasma intra-articular injection versus hyaluronic acid viscosupplementation as treatments for cartilage pathology: from early degeneration to osteoarthritis. Arthroscopy 2011;27:1490–1501.
    pubmed: 21831567
  5. Kon E, Buda R, Filardo G. Platelet-rich plasma: intra-articular knee injections produced favorable results on degenerative cartilage lesions. Knee Surg Sports Traumatol Arthrosc 2010;18:472–479.
    pubmed: 19838676
  6. Cole BJ, Karas V, Hussey K, Pilz K, Fortier LA. Hyaluronic acid versus platelet-rich plasma: a prospective, double-blind randomized controlled trial comparing clinical outcomes and effects on intra-articular biology for the treatment of knee osteoarthritis. Am J Sports Med 2017;45:339–346.
    pubmed: 28146403
  7. Broeckx S, Zimmerman M, Crocetti S. Regenerative therapies for equine degenerative joint disease: a preliminary study. PLoS One 2014;9:e85917.
    pmc: PMC3896436pubmed: 24465787
  8. Maia LSMV, Junior JIR, Oliveira AC, Alves GES, Benjamin LA, Silva YFRS, Zandim BM, Moreira JCL. Platelet-rich plasma in the treatment of induced of induced tendinopathy in horses: histologic evaluation. J Equine Vet Sci 2009;29:618–626.
  9. Sundman EA, Cole BJ, Karas V. The anti-inflammatory and matrix restorative mechanisms of platelet-rich plasma in osteoarthritis. Am J Sports Med 2014;42:35–41.
    pubmed: 24192391
  10. Russell RP, Apostolakos J, Hirose T, Cote MP, Mazzocca AD. Variability of platelet-rich plasma preparations. Sports Med Arthrosc 2013;21:186–190.
    pubmed: 24212365
  11. Kisiday JD, McIlwraith CW, Rodkey WG, Frisbie DD, Steadman JR. Effects of platelet-rich plasma composition on anabolic and catabolic activities in equine cartilage and meniscal explants. Cartilage 2012;3:245–254.
    pmc: PMC4297115pubmed: 26069637
  12. Braun HJ, Kim HJ, Chu CR, Dragoo JL. The effect of platelet-rich plasma formulations and blood products on human synoviocytes: implications for intra-articular injury and therapy. Am J Sports Med 2014;42:1204–1210.
    pmc: PMC5878923pubmed: 24634448
  13. Takafuji VA, McIlwraith CW, Howard RD. Effects of equine recombinant interleukin-1alpha and interleukin-1beta on proteoglycan metabolism and prostaglandin E2 synthesis in equine articular cartilage explants. Am J Vet Res 2002;63:551–558.
    pubmed: 11939318
  14. Little CB, Flannery CR, Hughes CE, Goodship A, Caterson B. Cytokine induced metalloproteinase expression and activity does not correlate with focal susceptibility of articular cartilage to degeneration. Osteoarthr Cartil 2005;13:162–170.
    pubmed: 15694578
  15. Caterson B, Flannery CR, Hughes CE, Little CB. Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biol 2000;19:333–344.
    pubmed: 10963994
  16. Carmona JU, Ríos DL, López C, Alvarez ME, Perez JE, Bohorquez ME. In vitro effects of platelet-rich gel supernatants on histology and chondrocyte apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide. BMC Vet Res 2016;12:135.
    pmc: PMC4929746pubmed: 27369779
  17. Osterman C, McCarthy MB, Cote MP, Beitzel K, Polkowski G, Mazzocca AD. Platelet-rich plasma increases anti-inflammatory markers in a human Coculture model for osteoarthritis. Am J Sports Med 2015;43:1474–1484.
    pubmed: 25716226
  18. Goldring MB, Marcu KB. Cartilage homeostasis in health and rheumatic diseases. Arthritis Res Ther 2009;11:224.
    pmc: PMC2714092pubmed: 19519926
  19. Filardo G, Kon E, Di Martino A, Di Matteo MML, Cenacchi A, Fornasari PM, Marcacci M. Platelet-rich plasma vs hyaluronic acid to treat knee degenerative pathology: study design and preliminary results of a randomized controlled trial. BMC Musculoskelet Disord 2012;13:229.
    pmc: PMC3532098pubmed: 23176112
  20. Textor JA, Tablin F. Activation of equine platelet-rich plasma: comparison of methods and characterization of equine autologous thrombin. Vet Surg 2012;41:784–794.
    pubmed: 22742830
  21. Roffi A, Filardo G, Assirelli E, Cavallo C, Cenacchi A, Facchini A, Grigolo B, Kon E, Mariani E, Pratelli L, Pulsatelli L, Marcacci M. Does platelet-rich plasma freeze-thawing influence growth factor release and their effects on chondrocytes and synoviocytes?. Biomed Res Int 2014;2014:692913.
    pmc: PMC4124719pubmed: 25136613
  22. Pietramaggiori G, Kaipainen A, Czeczuga JM, Wagner CT, Orgill DP. Freeze-dried platelet-ric. H plasma shows beneficial healing properties in chronic wounds. Wound Repair Regen 2006;14:573–580.
    pubmed: 17014669
  23. Creeper F, Ivanovski S. Effect of autologous and allogenic platelet-rich plasma on human gingival fibroblast function. Oral Dis 2012;18:494–500.
    pubmed: 22273115
  24. Zhang ZY, Huang AW, Fan JJ, Wei K, Jin D, Chen B, Li D, Bi L, Wang J, Pei G. The potential use of allogeneic platelet-rich plasma for large bone defect treatment: immunogenicity and defect healing efficacy. Cell Transplant 2013;22:175–187.
    pubmed: 22863146
  25. Yoshida R, Cheng M, Murray MM. Increasing platelet concentration in platelet-rich plasma inhibits anterior cruciate ligament cell function in three-dimensional culture. J Orthop Res 2014;32:291–295.
    pmc: PMC3945668pubmed: 24122902
  26. Weibrich G, Hansen T, Kleis W, Buch R, Hitzler WE. Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration. Bone 2004;34:665–671.
    pubmed: 15050897
  27. Weibrich G, Kleis WK, Hafner G, Hitzler WE. Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg 2002;30:97–102.
    pubmed: 12069512
  28. Ljungkvist M, Lövdahl S, Zetterberg E, Berntorp E. Low agreement between fresh and frozen-thawed platelet-rich plasma in the calibrated automated thrombogram assay. Haemophilia 2017;23:e214–e218.
    pubmed: 28145076
  29. Kisiday JD, Kurz B, DiMicco MA, Grodzinsky AJ. Evaluation of medium supplemented with insulin-transferrin-selenium for culture of primary bovine calf chondrocytes in three-dimensional hydrogel scaffolds. Tissue Eng 2005;11:141–151.
    pubmed: 15738669
  30. Kawcak CE, Trotter GW, Frisbie DD. Maintenance of equine articular cartilage explants in serum-free and serum-supplemented media, compared with that in a commercial supplemented medium. Am J Vet Res 1996;57(9):1261–1265.
    pubmed: 8874717
  31. McGowan KB, Kurtis MS, Lottman LM, Watson D, Sah RL. Biochemical quantification of DNA in human articular and septal cartilage using PicoGreen and Hoechst 33258. Osteoarthr Cartil 2002;10:580–587.
    pubmed: 12127839
  32. Kim YJ, Sah RL, Doong JY. Fluorometric assay of DNA in cartilage explants using Hoechst 33258. Anal Biochem 1988;174:168–176.
    pubmed: 2464289
  33. Farndale RW, Buttle DJ, Barrett AJ. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 1986;883:173–177.
    pubmed: 3091074
  34. Frisbie DD, Kawcak CE, Trotter GW. The assessment of chondrocyte proteoglycan metabolism using molecular sieve column chromatography as compared to three commonly utilized techniques. Osteoarthr Cartil 1998;6:137–145.
    pubmed: 9692068
  35. SAS/STAT 9.3 user's guide: SAS Institute Inc.; 2011.
Subscribe to our newsletter
Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.