FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Curr Res Parasitol Vector Borne Dis / Evaluation of buffered Trypanosoma evansi antigen and rapid ...
Current research in parasitology & vector-borne diseases2021; 1; 100024; doi: 10.1016/j.crpvbd.2021.100024

Evaluation of buffered Trypanosoma evansi antigen and rapid serum agglutination test (BA/Te) for the detection of anti-T. evansi antibodies in horses in Brazil.

Abstract: Surra is an infectious disease caused by , which affects a large number of domestic and wild animal species. Infection control is based on rapid diagnosis followed by treatment of sick animals. This study aimed to evaluate a buffered antigen and rapid serum agglutination test (BA/Te) for the detection of anti- antibodies in serum samples of horses. For this purpose, 445 serum samples from horses were evaluated and the results compared with the diagnosis by CATT/. Our data show a sensitivity of 92%, specificity of 91% and a degree of agreement kappa () of 0.82 (95% CI: 0.771-0.877,  < 0.01) between BA/Te and CATT/. Antigen specificity was also evaluated against reactive serum for other infectious agents circulating in equine herds. In conclusion, our findings show that BA/Te has the potential to be a practical and quick screening method for the detection of anti- antibodies in horses.
© 2021 The Author(s).
Get Full Text
Publication Date: 2021-04-24 PubMed ID: 35284895PubMed Central: PMC8906065DOI: 10.1016/j.crpvbd.2021.100024Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study aims to assess the effectiveness of the Buffered Antigen Trypanosoma evansi and rapid serum agglutination test (BA/Te) in detecting antibodies against the illness known as Surra in horses. The researchers found that the BA/Te test has promising results with a sensitivity of 92% and specificity of 91%.

Study Objective and Methodology

This research was designed to evaluate the BA/Te test’s effectiveness in detecting anti-T. evansi antibodies, a parasite causing Surra, in horse serum samples. Surra is a major infectious disease affecting various domestic and wild animal species, with the key to controlling it being prompt diagnosis and treatment.

  • The researchers tested 445 horse serum samples using the BA/Te, comparing the results with those obtained from the CATT/T. evansi diagnosis – a commonly used method for detecting this disease.
  • Furthermore, the antigen specificity of BA/Te was evaluated against serum reactive to other infectious agents present in horse herds to gauge its ability to distinguish the specific disease-causing agent.

Findings and Conclusion

The outcomes of this study demonstrate a successful application of the BA/Te test in diagnosing Surra. The key results include:

  • The BA/Te test revealed a sensitivity of 92%, indicating a high ability to correctly identify horses infected with Surra.
  • The specificity was 91%, suggesting a high capability to correctly identify healthy horses or those free of Surra.
  • The degree of agreement (kappa) between BA/Te and CATT/T. evansi was 0.82 (with a 95% Confidence Interval: 0.771-0.877), implying a very good agreement between the two tests.
  • The antigen specificity of BA/Te against other circulating infectious agents in horse herds demonstrated its value in tackling diagnostic confusion that might arise from cross-reactivity.

Therefore, the research concludes that BA/Te could offer a practical and quick screening method for the detection of anti-T. evansi antibodies in horses and play a crucial role in controlling Surra. The higher sensitivity and specificity values provide assurance in the test’s potential to be a reliable diagnostic tool. The speed and simplicity of this method might lead to its broader adoption in similar scenarios across veterinary medicine.

Cite This Article

APA
Reck C, Menin Á, Batista F, Meira Santos PO, Miletti LC. (2021). Evaluation of buffered Trypanosoma evansi antigen and rapid serum agglutination test (BA/Te) for the detection of anti-T. evansi antibodies in horses in Brazil. Curr Res Parasitol Vector Borne Dis, 1, 100024. https://doi.org/10.1016/j.crpvbd.2021.100024

Publication

Current research in parasitology & vector-borne diseases
ISSN: 2667-114X
NlmUniqueID: 9918226380706676
Country: Netherlands
Language: English
Volume: 1
Pages: 100024
PII: 100024

Researcher Affiliations

Reck, Carolina
  • Laboratório de Bioquímica de Hemoparasitas e Vetores, Departamento de Produção Animal e Alimentos, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Lages, SC, Brazil.
  • VERTÁ - Laboratory of Veterinary Diagnostic / Institute of Veterinary Research and Diagnostic, Av. Lions, 1286 - Bairro Nossa Sra. Aparecida, Curitibanos, SC 89520-000, Brazil.
Menin, Álvaro
  • Departmento de Biociências e Saúde Única, Universidade Federal de Santa Catarina, Rodovia Ulysses Gaboardi, 3000, Km 3, Curitibanos, SC 89520-000, Brazil.
Batista, Franciane
  • Laboratório de Bioquímica de Hemoparasitas e Vetores, Departamento de Produção Animal e Alimentos, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Lages, SC, Brazil.
Meira Santos, Patricia Oliveira
  • Centro de Ciências Agrárias, Universidade Federal de Sergipe, Av. Marechal Rondon, Cidade Universitária, Jardim Rosa Elze, 49100-000 São Cristóvão, SE, Brazil.
Miletti, Luiz Claudio
  • Laboratório de Bioquímica de Hemoparasitas e Vetores, Departamento de Produção Animal e Alimentos, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Lages, SC, Brazil.

Conflict of Interest Statement

The authors declare that they have no competing interests.

References

This article includes 24 references
  1. Altman D.G.. PracticalStatistics forMedicalResearch. 1990.
  2. Aquino LP, Machado RZ, Lemos KR, Marques LC, Garcia MV, Borges GP. Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis.. Rev Bras Parasitol Vet 2010 Apr-Jun;19(2):112-8.
    doi: 10.4322/rbpv.01902009pubmed: 20624349google scholar: lookup
  3. Bajyana Songa E, Hamers R. A card agglutination test (CATT) for veterinary use based on an early VAT RoTat 1/2 of Trypanosoma evansi.. Ann Soc Belg Med Trop 1988 Sep;68(3):233-40.
    pubmed: 3223785
  4. Claes F, Radwanska M, Urakawa T, Majiwa PA, Goddeeris B, Büscher P. Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections.. Kinetoplastid Biol Dis 2004 Sep 17;3(1):3.
    doi: 10.1186/1475-9292-3-3pmc: PMC521498pubmed: 15377385google scholar: lookup
  5. Colpo C.B., Monteiro S.G., Stainki D.R., Colpo E.T.B., Henriques G.B.. Infecção natural por Trypanosoma evansi em cães. Ciência Rural 2005;35:717–719.
  6. Corbel MJ. Identification of the immunoglobulin class active in the Rose Bengal plate test for bovine brucellosis.. J Hyg (Lond) 1972 Dec;70(4):779-95.
    doi: 10.1017/s0022172400022622pmc: PMC2130279pubmed: 4630606google scholar: lookup
  7. Desquesnes M.. Livestock trypanosomoses and their vectors in Latin America. 2004.
  8. Desquesnes M, Dargantes A, Lai DH, Lun ZR, Holzmuller P, Jittapalapong S. Trypanosoma evansi and surra: a review and perspectives on transmission, epidemiology and control, impact, and zoonotic aspects.. Biomed Res Int 2013;2013:321237.
    doi: 10.1155/2013/321237pmc: PMC3789323pubmed: 24151595google scholar: lookup
  9. Desquesnes M, Holzmuller P, Lai DH, Dargantes A, Lun ZR, Jittaplapong S. Trypanosoma evansi and surra: a review and perspectives on origin, history, distribution, taxonomy, morphology, hosts, and pathogenic effects.. Biomed Res Int 2013;2013:194176.
    doi: 10.1155/2013/194176pmc: PMC3760267pubmed: 24024184google scholar: lookup
  10. Grab DJ, Bwayo JJ. Isopycnic isolation of African trypanosomes on Percoll gradients formed in situ.. Acta Trop 1982 Dec;39(4):363-6.
    pubmed: 6131595
  11. Hilali M, Abdel-Gawad A, Nassar A, Abdel-Wahab A, Magnus E, Büscher P. Evaluation of the card agglutination test (CATT/T. evansi) for detection of Trypanosoma evansi infection in water buffaloes (Bubalus bubalis) in Egypt.. Vet Parasitol 2004 May 7;121(1-2):45-51.
    doi: 10.1016/j.vetpar.2004.02.009pubmed: 15110402google scholar: lookup
  12. Jones TW, McKinnell CD. Antigenic variation in Trypanosoma evansi: variable antigen type development in mice, sheep and goats.. Trop Med Parasitol 1985 Mar;36(1):53-7.
    pubmed: 4001768
  13. Laha R, Sasmal NK. Characterization of immunogenic proteins of Trypanosoma evansi isolated from three different Indian hosts using hyperimmune sera and immune sera.. Res Vet Sci 2008 Dec;85(3):534-9.
    doi: 10.1016/j.rvsc.2008.02.011pubmed: 18371998google scholar: lookup
  14. Lanham SM, Godfrey DG. Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.. Exp Parasitol 1970 Dec;28(3):521-34.
    doi: 10.1016/0014-4894(70)90120-7pubmed: 4993889google scholar: lookup
  15. Lun ZR, Fang Y, Wang CJ, Brun R. Trypanosomiasis of domestic animals in China.. Parasitol Today 1993 Feb;9(2):41-5.
    doi: 10.1016/0169-4758(93)90029-fpubmed: 15463701google scholar: lookup
  16. Njiru ZK, Ouma JO, Enyaru JC, Dargantes AP. Loop-mediated Isothermal Amplification (LAMP) test for detection of Trypanosoma evansi strain B.. Exp Parasitol 2010 Jul;125(3):196-201.
    doi: 10.1016/j.exppara.2010.01.017pubmed: 20109454google scholar: lookup
  17. Patterson JM, Deyoe BL, Stone SS. Identification of immunoglobulins associated with complement fixation, agglutination, and low pH buffered antigen tests for brucellosis.. Am J Vet Res 1976 Mar;37(3):319-24.
    pubmed: 3997
  18. Rodrigues A, Fighera RA, Souza TM, Schild AL, Barros CS. Neuropathology of naturally occurring Trypanosoma evansi infection of horses.. Vet Pathol 2009 Mar;46(2):251-8.
    doi: 10.1354/vp.46-2-251pubmed: 19261636google scholar: lookup
  19. Sharma P, Juyal PD, Singla LD, Chachra D, Pawar H. Comparative evaluation of real time PCR assay with conventional parasitological techniques for diagnosis of Trypanosoma evansi in cattle and buffaloes.. Vet Parasitol 2012 Dec 21;190(3-4):375-82.
    doi: 10.1016/j.vetpar.2012.07.005pubmed: 22858228google scholar: lookup
  20. Silva RA, Arosemena NA, Herrera HM, Sahib CA, Ferreira MS. Outbreak of trypanosomosis due to Trypanosoma evansi in horses of Pantanal Mato-grossense, Brazil.. Vet Parasitol 1995 Nov;60(1-2):167-71.
    doi: 10.1016/0304-4017(94)00757-4pubmed: 8644453google scholar: lookup
  21. Singla L.D., Sharma A., Kaur P., Bal M.S.. Comparative evaluation of agglutination assay with microscopy and polymerase chain reaction for detection of Trypanosoma evansi in bovines of Punjab. Ind. J. Anim. Sci. 2015;85:1164–1166.
  22. Sivajothi S, Rayulu VC, Bhaskar Reddy BV, Malakondaiah P, Sreenivasulu D, Sudhakara Reddy B. Polypeptide profiles of South Indian isolate of Trypanosoma evansi.. J Parasit Dis 2016 Sep;40(3):652-5.
    pmc: PMC4996165pubmed: 27605761doi: 10.1007/s12639-014-0552-1google scholar: lookup
  23. Thekisoe OM, Inoue N, Kuboki N, Tuntasuvan D, Bunnoy W, Borisutsuwan S, Igarashi I, Sugimoto C. Evaluation of loop-mediated isothermal amplification (LAMP), PCR and parasitological tests for detection of Trypanosoma evansi in experimentally infected pigs.. Vet Parasitol 2005 Jun 30;130(3-4):327-30.
    pubmed: 15908123doi: 10.1016/j.vetpar.2005.04.019google scholar: lookup
  24. Zayed A.A., Habeeb S.M., Allam N.A.T., Ashry H.M.Z., Mohamed A.H.H., Ashour A.A., Taha H.A.. A critical comparative study of parasitological and serological differential diagnostic methods of Trypanosoma evansi infections in some farm animals in Egypt. Am.-Euras. J. Agricul. Environ. Sci. 2010;8:633–642.

Citations

This article has been cited 0 times.
Subscribe to our newsletter
Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.