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Current research in parasitology & vector-borne diseases2021; 1; 100024; doi: 10.1016/j.crpvbd.2021.100024

Evaluation of buffered Trypanosoma evansi antigen and rapid serum agglutination test (BA/Te) for the detection of anti-T. evansi antibodies in horses in Brazil.

Abstract: Surra is an infectious disease caused by , which affects a large number of domestic and wild animal species. Infection control is based on rapid diagnosis followed by treatment of sick animals. This study aimed to evaluate a buffered antigen and rapid serum agglutination test (BA/Te) for the detection of anti- antibodies in serum samples of horses. For this purpose, 445 serum samples from horses were evaluated and the results compared with the diagnosis by CATT/. Our data show a sensitivity of 92%, specificity of 91% and a degree of agreement kappa () of 0.82 (95% CI: 0.771-0.877,  < 0.01) between BA/Te and CATT/. Antigen specificity was also evaluated against reactive serum for other infectious agents circulating in equine herds. In conclusion, our findings show that BA/Te has the potential to be a practical and quick screening method for the detection of anti- antibodies in horses.
© 2021 The Author(s).
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Publication Date: 2021-04-24 PubMed ID: 35284895PubMed Central: PMC8906065DOI: 10.1016/j.crpvbd.2021.100024Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study aims to assess the effectiveness of the Buffered Antigen Trypanosoma evansi and rapid serum agglutination test (BA/Te) in detecting antibodies against the illness known as Surra in horses. The researchers found that the BA/Te test has promising results with a sensitivity of 92% and specificity of 91%.

Study Objective and Methodology

This research was designed to evaluate the BA/Te test’s effectiveness in detecting anti-T. evansi antibodies, a parasite causing Surra, in horse serum samples. Surra is a major infectious disease affecting various domestic and wild animal species, with the key to controlling it being prompt diagnosis and treatment.

  • The researchers tested 445 horse serum samples using the BA/Te, comparing the results with those obtained from the CATT/T. evansi diagnosis – a commonly used method for detecting this disease.
  • Furthermore, the antigen specificity of BA/Te was evaluated against serum reactive to other infectious agents present in horse herds to gauge its ability to distinguish the specific disease-causing agent.

Findings and Conclusion

The outcomes of this study demonstrate a successful application of the BA/Te test in diagnosing Surra. The key results include:

  • The BA/Te test revealed a sensitivity of 92%, indicating a high ability to correctly identify horses infected with Surra.
  • The specificity was 91%, suggesting a high capability to correctly identify healthy horses or those free of Surra.
  • The degree of agreement (kappa) between BA/Te and CATT/T. evansi was 0.82 (with a 95% Confidence Interval: 0.771-0.877), implying a very good agreement between the two tests.
  • The antigen specificity of BA/Te against other circulating infectious agents in horse herds demonstrated its value in tackling diagnostic confusion that might arise from cross-reactivity.

Therefore, the research concludes that BA/Te could offer a practical and quick screening method for the detection of anti-T. evansi antibodies in horses and play a crucial role in controlling Surra. The higher sensitivity and specificity values provide assurance in the test’s potential to be a reliable diagnostic tool. The speed and simplicity of this method might lead to its broader adoption in similar scenarios across veterinary medicine.

Cite This Article

APA
Reck C, Menin Á, Batista F, Meira Santos PO, Miletti LC. (2021). Evaluation of buffered Trypanosoma evansi antigen and rapid serum agglutination test (BA/Te) for the detection of anti-T. evansi antibodies in horses in Brazil. Curr Res Parasitol Vector Borne Dis, 1, 100024. https://doi.org/10.1016/j.crpvbd.2021.100024

Publication

Current research in parasitology & vector-borne diseases
ISSN: 2667-114X
NlmUniqueID: 9918226380706676
Country: Netherlands
Language: English
Volume: 1
Pages: 100024
PII: 100024

Researcher Affiliations

Reck, Carolina
  • Laboratório de Bioquímica de Hemoparasitas e Vetores, Departamento de Produção Animal e Alimentos, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Lages, SC, Brazil.
  • VERTÁ - Laboratory of Veterinary Diagnostic / Institute of Veterinary Research and Diagnostic, Av. Lions, 1286 - Bairro Nossa Sra. Aparecida, Curitibanos, SC 89520-000, Brazil.
Menin, Álvaro
  • Departmento de Biociências e Saúde Única, Universidade Federal de Santa Catarina, Rodovia Ulysses Gaboardi, 3000, Km 3, Curitibanos, SC 89520-000, Brazil.
Batista, Franciane
  • Laboratório de Bioquímica de Hemoparasitas e Vetores, Departamento de Produção Animal e Alimentos, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Lages, SC, Brazil.
Meira Santos, Patricia Oliveira
  • Centro de Ciências Agrárias, Universidade Federal de Sergipe, Av. Marechal Rondon, Cidade Universitária, Jardim Rosa Elze, 49100-000 São Cristóvão, SE, Brazil.
Miletti, Luiz Claudio
  • Laboratório de Bioquímica de Hemoparasitas e Vetores, Departamento de Produção Animal e Alimentos, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Lages, SC, Brazil.

Conflict of Interest Statement

The authors declare that they have no competing interests.

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Citations

This article has been cited 1 times.
  1. Sousa JA, Miranda LM, Coutinho DJB, Costa TF, Costa SP, Freitas ÚS, Costa FB, Machado RZ, Nogueira RMS, Costa APD. Trypanosoma evansi in Brazil: first evidence of infection in equines from Northeast region. Rev Bras Parasitol Vet 2025;34(2):e000125.
    doi: 10.1590/S1984-29612025032pubmed: 40561341google scholar: lookup
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