Evaluation of concurrent vaccinations with recombinant canarypox equine influenza virus and inactivated equine herpesvirus vaccines.
Abstract: Despite vaccination, equine influenza virus (EIV) and equine herpesvirus (EHV) infections still cause highly contagious respiratory diseases in horses. Recently, concurrent vaccination with EIV and EHV was suggested as a new approach; however, there have been no reports of concurrent vaccination with recombinant canarypox EIV and inactivated EHV vaccines. In this study, we aimed to compare the EIV-specific immune responses induced by concurrent administrations of a recombinant canarypox EIV vaccine and an inactivated bivalent EHV vaccine with those induced by a single recombinant canarypox EIV vaccine in experimental horse and mouse models. Serum and peripheral blood mononuclear cells (PBMCs) were collected from immunized animals after vaccination. EIV-specific serum antibody levels, serum hemagglutinin inhibition (HI) titers, and interferon-gamma (IFN-γ) levels were measured by enzyme-linked immunosorbent assay, HI assay, and quantitative polymerase chain reaction, respectively. Concurrent EIV and EHV vaccine administration significantly increased IFN-γ production, without compromising humoral responses. Our data demonstrate that concurrent vaccination with EIV and EHV vaccines can enhance EIV-specific cellular responses in horses.
© Copyright 2022 Korean Society of Animal Science and Technology.
Publication Date: 2022-05-31 PubMed ID: 35709134PubMed Central: PMC9184697DOI: 10.5187/jast.2022.e30Google Scholar: Lookup
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- Journal Article
- Animal Studies
- Disease Treatment
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Equine Diseases
- Equine Health
- Equine Herpesvirus
- Equine model
- Experimental Methods
- Immune Response
- Immunization
- Immunology
- In Vivo
- Infectious Disease
- Influenza
- Polymerase Chain Reaction
- Serum
- Vaccine
- Vaccine development
- Veterinary Medicine
- Virology
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article discusses a study conducted on equine influenza virus (EIV) and equine herpesvirus (EHV), investigating the effects of concurrent vaccinations using a recombinant canarypox EIV vaccine and an inactivated bivalent EHV vaccine in horse and mouse models.
Objectives
- The researchers aimed to compare the EIV-specific immune responses induced by concurrent administrations of a recombinant canarypox EIV vaccine and an inactivated bivalent EHV vaccine with those induced by a standalone recombinant canarypox EIV vaccine.
Methods
- The team used both horse and mouse models for their experiments.
- After administering the vaccines, they collected Serum and peripheral blood mononuclear cells (PBMCs) from the immunized animals.
- Using these samples, they measured EIV-specific serum antibody levels, serum hemagglutinin inhibition (HI) titers, and interferon-gamma (IFN-γ) levels.
- The antibody levels and HI titers were measured using enzyme-linked immunosorbent assay and HI assay, while IFN-γ levels were determined using quantitative polymerase chain reaction.
Results
- From their findings, the researchers observed that concurrent administration of the EIV and EHV vaccines increased the production of IFN-γ significantly.
- Interestingly, this increase did not compromise the humoral responses – the part of the immune system responsible for producing antibodies.
Conclusion
- The research suggests that concurrent vaccination with EIV and EHV vaccines can enhance the EIV-specific cellular responses in horses.
- However, these findings must be validated through further research before this dual-vaccination approach can be integrated into practical applications.
Cite This Article
APA
Lee DH, Lee EB, Seo JP, Ko EJ.
(2022).
Evaluation of concurrent vaccinations with recombinant canarypox equine influenza virus and inactivated equine herpesvirus vaccines.
J Anim Sci Technol, 64(3), 588-598.
https://doi.org/10.5187/jast.2022.e30 Publication
Researcher Affiliations
- College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Korea.
- Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea.
- Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University, Jeju 63243, Korea.
- College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Korea.
Conflict of Interest Statement
No potential conflict of interest relevant to this article was reported.
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Citations
This article has been cited 1 times.- Lee DH, Lee EB, Seo JP, Ko EJ. In vitro effects of monophosphoryl lipid A and Poly I:C combination on equine cells.. J Vet Sci 2023 May;24(3):e37.
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