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Drug testing and analysis2020; 13(2); 386-396; doi: 10.1002/dta.2934

Evaluation of dried blood spots as an alternative sample matrix for equine antidoping analysis.

Abstract: Controlling the abuse of prohibited substances such as anabolic steroids, selective androgen receptor modulators, β-adrenoceptor agonists, and blood doping agents is of great interest to racing authorities. The use of dried blood spots (DBS) as an alternative sampling approach may be a feasible approach for controlling the use of these agents. To assess the feasibility of using DBS in equine blood, an 11-min liquid chromatography-mass spectrometry method was developed on a triple quadrupole mass spectrometer following extraction from Whatman 903 DBS cards. A total of 50 compounds across multiple compound classes were detectable with reproducible results. The stability was assessed with good results after almost 3 months of storage at ambient temperatures. These results suggest that the use of DBS may be a feasible alternative sampling approach in equine drug testing.
© 2020 John Wiley & Sons, Ltd.
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Publication Date: 2020-10-19 PubMed ID: 33001574DOI: 10.1002/dta.2934Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about the evaluation of dried blood spots (DBS) as a potential alternative method for testing prohibited substances in equines (horses) for anti-doping purposes.

Introduction

  • The article begins with the researchers explaining why it’s important to control the use of prohibited substances like anabolic steroids and blood doping agents in racehorses. Their inappropriate usage might give unfair advantages in racing and may be harmful to the horse’s health.
  • To enhance the process of doping tests, the researchers proposed the use of dried blood spots (DBS) as an alternative sampling method.

Methodology

  • The researchers developed an 11-minute liquid chromatography-mass spectrometry method for the proposed sampling process. This involved extraction from Whatman 903 DBS cards.
  • This scientific method combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometry, which helps in the identification of different compounds in the sample.

Results

  • A total of 50 compounds across various classes were detectable with this method, and the results were reproducible, validating the effectiveness of the method.

Stability Test

  • The researchers then assessed the stability of the DBS. The samples were found to be stable even after being stored at normal room temperatures for approximately 3 months.

Conclusion

  • Based on the study’s findings, the authors concluded that the use of dried blood spots might be a viable alternative sampling method for equine drug testing.

Cite This Article

APA
Moeller BC, Yang Z. (2020). Evaluation of dried blood spots as an alternative sample matrix for equine antidoping analysis. Drug Test Anal, 13(2), 386-396. https://doi.org/10.1002/dta.2934

Publication

Drug testing and analysis
ISSN: 1942-7611
NlmUniqueID: 101483449
Country: England
Language: English
Volume: 13
Issue: 2
Pages: 386-396

Researcher Affiliations

Moeller, Benjamin C
  • Kenneth L. Maddy Equine Analytical Chemistry Laboratory, School of Veterinary Medicine, University of California Davis, Davis, CA, USA.
Yang, Zicheng
  • Mass Spectrometry Deptartment, Bruker Scientific LLC, San Jose, CA, USA.

MeSH Terms

  • Anabolic Agents / blood
  • Androgens / blood
  • Animals
  • Chromatography, Liquid / methods
  • Doping in Sports
  • Dried Blood Spot Testing / methods
  • Horses / blood
  • Limit of Detection
  • Mass Spectrometry / methods
  • Steroids / blood
  • Substance Abuse Detection / methods

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This article includes 19 references
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Citations

This article has been cited 1 times.
  1. Samsonova JV, Saushkin NY, Osipov AP. Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives. Vet Res Commun 2022 Sep;46(3):655-698.
    doi: 10.1007/s11259-022-09957-wpubmed: 35771305google scholar: lookup
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