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Journal of animal science2020; 98(3); skaa050; doi: 10.1093/jas/skaa050

Evaluation of equine rectal inoculum as representative of the microbial activities within the horse hindgut using a fully automated in vitro gas production technique system.

Abstract: The in vitro gas production technique (IVGPT) has been a valuable tool in ruminant nutrition research for decades and has more recently been used in horse nutrition studies to investigate fermentation activities of the equine hindgut though primarily using feces as inoculum. This study was conducted to evaluate the use of equine rectal content in the IVGPT system as a viable inoculum that can be considered representative of the activities throughout the equine hindgut. Additionally, the study was conducted to measure the effects on fermentation kinetics and end-product production using inoculum from horses fed supplemental levels of coated sodium butyrate in an IVGPT system. Eight warmblood horses were fed a diet consisting of haylage (1% DM intake based on ideal body weight [BW]) and a mash concentrate formulated to provide 2.5 g nonstructural carbohydrate (NSC)/kg BW per meal. The diet was intended to create a NSC challenge to the microbial populations of the hindgut. The horses were randomly assigned to treatment or control group and after a 1-wk diet-adaptation period, the treatment group received 0.4 g/kg BW per day of a coated sodium butyrate supplement, while the control group received a placebo (coating only). After a 3-wk treatment period, the animals were sacrificed and digesta from the cecum, left ventral colon, right dorsal colon, and the rectum were collected within 30 min postmortem and used as inocula for the IVGPT trial. Haylage and concentrates fed to the test animals were also used as substrates in vitro. Sodium butyrate supplementation was not significant for gas production parameters or VFA measured suggesting no effect of sodium butyrate supplementation on the extent or kinetics of gas production or microbial end-product production (P ≥ 0.073). Differences in inocula were significant for organic matter corrected cumulative gas production (P = 0.0001), asymptotic gas production of the second phase (A2) (P < 0.0001); and maximal rate of OM degradation of the second phase (Rmax2) (P = 0.002). Inocula had a significant effect on total VFA (P = 0.0002), butyrate (Bu) (P = 0.015), branched chain fatty acids (P < 0.0001), pH (P < 0.0001), and ammonia (NH3) (P = 0.0024). In conclusion, based on observed results from this study, total tract digestibility may be overestimated if using rectal content inoculum to evaluate forage-based feeds in an IVGPT system.
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Publication Date: 2020-02-23 PubMed ID: 32076715PubMed Central: PMC7097714DOI: 10.1093/jas/skaa050Google Scholar: Lookup
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Summary

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This research article studies the use of the in vitro gas production technique (IVGPT) in horse nutrition research, specifically to investigate the fermentation activities within the equine hindgut. Through the evaluation of rectal inoculum from horses, the study measures the effects of sodium butyrate supplementation on fermentation kinetics and end-product production, and concludes that total tract digestibility may be overestimated if the rectal content is used as an inoculum.

Objective and Methodology of the Research

  • The study aimed at evaluating if an equine’s rectal content, when used with an in vitro gas production technique (IVGPT), could represent the activities within the horse’s hindgut. It also investigated the impact of sodium butyrate supplement when included in the horse’s diet on fermentation kinetics and end-product production.
  • Eight warmblood horses were selected, where they were served haylage and a mash concentrate to challenge the microbial populations in the hindgut. Later, they were randomized into two groups, one which received a sodium butyrate supplement, and a control group given a placebo.
  • After three weeks, the horses were sacrificed and their organisms’ digestive content, from the cecum, left ventral colon, right dorsal colon, and the rectum, were collected for the IVGPT trial.

Findings of the Study

  • The study found that sodium butyrate supplementation had no significant effect on gas production parameters, voluntary feed intake, or the extent or kinetics of gas production and microbial end-product production.
  • Significant differences were noted in the organic matter corrected cumulative gas production, asymptotic gas production, and the maximal rate of organic matter degradation between the different inocula gathered.
  • The inocula also had a significant influence on total volatile fatty acids, butyrate, branched chain fatty acids, pH, and ammonia.
  • The research concluded that if rectal content inoculum is used to evaluate forage-based feeds in an IVGPT system, there could be an overestimation of total tract digestibility.

Significance and Conclusions

  • This research is significant as it throws light on the validity and effectiveness of yield results when using rectal inoculum in the in vitro gas production technique system. It also helps understand the influence of sodium butyrate supplementation on the equine diet.
  • While the inclusion of sodium butyrate does not markedly alter fermentation kinetics, rectal content inoculum could possibly give an overestimated measure of the entire tract’s digestibility in an IVGPT system.

Cite This Article

APA
Kujawa TJ, van Doorn DA, Wambacq WA, Hesta M, Pellikaan WF. (2020). Evaluation of equine rectal inoculum as representative of the microbial activities within the horse hindgut using a fully automated in vitro gas production technique system. J Anim Sci, 98(3), skaa050. https://doi.org/10.1093/jas/skaa050

Publication

Journal of animal science
ISSN: 1525-3163
NlmUniqueID: 8003002
Country: United States
Language: English
Volume: 98
Issue: 3
PII: skaa050

Researcher Affiliations

Kujawa, Theresa J
  • Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
  • Department of Animal Sciences, Animal Nutrition Group, Wageningen University & Research, Wageningen, The Netherlands.
van Doorn, David A
  • Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
  • Department of Equine Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Wambacq, Wendy A
  • Laboratory of Animal Nutrition, Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Hesta, Myriam
  • Laboratory of Animal Nutrition, Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Pellikaan, Wilbert F
  • Department of Animal Sciences, Animal Nutrition Group, Wageningen University & Research, Wageningen, The Netherlands.

MeSH Terms

  • Animals
  • Butyric Acid / analysis
  • Diet / veterinary
  • Dietary Supplements / analysis
  • Digestion
  • Fatty Acids, Volatile / analysis
  • Fatty Acids, Volatile / metabolism
  • Feces / microbiology
  • Fermentation
  • Gastrointestinal Microbiome / physiology
  • Gastrointestinal Tract / metabolism
  • Gastrointestinal Tract / microbiology
  • Horses / metabolism
  • Horses / microbiology
  • In Vitro Techniques / veterinary
  • Random Allocation
  • Rectum / metabolism
  • Rectum / microbiology

References

This article includes 39 references
  1. Akhter S, Owen E, Theodorou M K, Butler E A, Minson D J. Bovine faeces as a source of micro-organisms for the in vitro digestibility assay of forages.. Grass Forage Sci. 1999 54:219–226.
    doi: 10.1046/j.1365-2494.1999.00174.xgoogle scholar: lookup
  2. Biagi G, Piva A, Moschini M, Vezzali E, Roth FX. Performance, intestinal microflora, and wall morphology of weanling pigs fed sodium butyrate.. J Anim Sci 2007 May;85(5):1184-91.
    doi: 10.2527/jas.2006-378pubmed: 17296766google scholar: lookup
  3. Carraro L, Xiccato G, Trocino A, Radaelli G. Dietary supplementation of butyrate in growing rabbits.. It. J. Anim. Sci. 2005 4(Suppl. 2):538–540.
    doi: 10.4081/ijas.2005.2s.538google scholar: lookup
  4. Clarke LL, Roberts MC, Argenzio RA. Feeding and digestive problems in horses. Physiologic responses to a concentrated meal.. Vet Clin North Am Equine Pract 1990 Aug;6(2):433-50.
    doi: 10.1016/s0749-0739(17)30550-3pubmed: 2202501google scholar: lookup
  5. Cone J W, Van Gelder A H, Driehuis F. Description of gas production profiles with a three-phasic model.. Anim. Feed Sci. Technol. 1997 66:31–45.
    doi: 10.1016/S0377-8401(96)01147-9google scholar: lookup
  6. Cone J W, Van Gelder A H, Visscher G J W, Oudshoorn L. Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus.. Anim. Feed Sci. Technol. 1996 61:113–128.
    doi: 10.1016/0377-8401(96)00950-9google scholar: lookup
  7. Desrousseaux G, Santos A G, Pellikaan W F, Van der Poel A F B, Cone J W, Ferreira L M M, Guedes C M V, Rodrigues M A M. Effect of collection time on the fermentative activity of microbes in equine feces.. Anim. Feed. Sci. Technol. 2012 178:183–189.
    doi: 10.1016/j.anifeedsci.2012.09.016google scholar: lookup
  8. Garner HE, Moore JN, Johnson JH, Clark L, Amend JF, Tritschler LG, Coffmann JR, Sprouse RF, Hutcheson DP, Salem CA. Changes in the caecal flora associated with the onset of laminitis.. Equine Vet J 1978 Oct;10(4):249-52.
    doi: 10.1111/j.2042-3306.1978.tb02273.xpubmed: 738266google scholar: lookup
  9. Goodson J, Tyznik WJ, Cline JH, Dehority BA. Effects of an abrupt diet change from hay to concentrate on microbial numbers and physical environment in the cecum of the pony.. Appl Environ Microbiol 1988 Aug;54(8):1946-50.
    pmc: PMC202784pubmed: 3178206doi: 10.1128/aem.54.8.1946-1950.1988google scholar: lookup
  10. Górka P, Kowalski ZM, Pietrzak P, Kotunia A, Jagusiak W, Holst JJ, Guilloteau P, Zabielski R. Effect of method of delivery of sodium butyrate on rumen development in newborn calves.. J Dairy Sci 2011 Nov;94(11):5578-88.
    doi: 10.3168/jds.2011-4166pubmed: 22032381google scholar: lookup
  11. Groot J C J, Cone J W, Williams B A W, Debersaques F M A, Lantinga E A. Multiphasic analysis of gas production kinetics for in vitro fermentation of ruminant feeds.. Anim. Feed Sci. Technol. 1996 64:77–89.
    doi: 10.1016/S0377-8401(96)01012-7google scholar: lookup
  12. Hassanin A, Tony M A, Sawiress F A R, Abdl-Rahman M A, Sohair Saleh Y. Influence of dietary supplementation of coated sodium butyrate and/or synbiotic on growth performances, cecal fermentation, intestinal morphometry and metabolic profile of growing rabbits.. J. Agric. Sci. 2015 7:239–252.
    doi: 10.5539/jas.v7n2p180google scholar: lookup
  13. Hastie PM, Mitchell K, Murray JA. Semi-quantitative analysis of Ruminococcus flavefaciens, Fibrobacter succinogenes and Streptococcus bovis in the equine large intestine using real-time polymerase chain reaction.. Br J Nutr 2008 Sep;100(3):561-8.
    doi: 10.1017/S0007114508968227pubmed: 18377691google scholar: lookup
  14. Henneke DR, Potter GD, Kreider JL, Yeates BF. Relationship between condition score, physical measurements and body fat percentage in mares.. Equine Vet J 1983 Oct;15(4):371-2.
    doi: 10.1111/j.2042-3306.1983.tb01826.xpubmed: 6641685google scholar: lookup
  15. Hintz HF, Argenzio RA, Schryver HF. Digestion coefficients, blood glucose levels and molar percentage of volatile acids in intestinal fluid of ponies fed varying forage-grain ratios.. J Anim Sci 1971 Nov;33(5):992-5.
    doi: 10.2527/jas1971.335992xpubmed: 5119977google scholar: lookup
  16. Hu Z, Guo Y. Effects of dietary sodium butyrate supplementation on the intestinal morphological structure, absorptive function and gut flora in chickens.. Anim. Feed Sci. Technol. 2007 132:240–249.
    doi: 10.1016/j.anifeedsci.2006.03.017google scholar: lookup
  17. Huyen N T, Fryganas C, Uittenbogaard G, Mueller-Harvey I, Verstegen M W A, Hendriks W H, Pellikaan W F. Structural features of condensed tannins affect in vitro ruminal methane production and fermentation characteristics.. J. Agric. Sci. 2016 154:1474–1487.
    doi: 10.1017/S0021859616000393google scholar: lookup
  18. Lowman R S. Investigations into the factors which influence measurements during in vitro gas production studies [Ph.D. thesis].. 1998.
  19. Lowman R S, Theodorou M K, Hyslop J J, Dhanoa M S, Cí·¯ord D. Evaluation of an in vitro batch culture technique for estimating the in vitro digestibility and digestible energy content of equine feeds using equine faeces as the source of microbial inoculum.. Anim. Feed Sci. Techol. 1999 80:11–27.
    doi: 10.1016/S0377-8401(99)00039-5google scholar: lookup
  20. Lu J J, Zou X T, Wang Y M. Effects of sodium butyrate on the growth performance, intestinal microflora and morphology of weanling pigs.. J. Anim. Feed Sci. 2008 17:568–578.
    doi: 10.22358/jafs/66685/2008google scholar: lookup
  21. Mauricio R M, Mould F, Owen E. Comparison of rumen liquor and faeces from cows as sources of micro-organisms for the in vitro gas production technique.. Arq. Bras. Med. Vet. Zootec. 1998 50:569–572.
  22. Mauricio R M, Owen E, Mould F L, Givens I, Theodorou M K, France J, Davies D R, Dhanoa M S. Comparison of bovine rumen liquor and bovine faeces as inoculum for an in vitro gas production technique for evaluating forages.. Anim. Feed Sci. Technol. 2001 89:33–48.
    doi: 10.1016/S0377-8401(00)00234-0google scholar: lookup
  23. Murray J-A M D, Longland A C, Moore-Colyer M J S. In vitro fermentation of different ratios of high-temperature dried lucerne and sugar beet pulp incubated with an equine faecal inoculum.. Anim. Feed Sci. Technol. 2005 129:89–98.
    doi: 10.1016/j.anifeedsci.2005.12.001google scholar: lookup
  24. Murray JA, Longland AC, Moore-Colyer MJ, Dunnett C. The effect of enzyme treatment on the in vitro fermentation of lucerne incubated with equine faecal inocula.. Br J Nutr 2005 Nov;94(5):771-82.
    doi: 10.1079/bjn20051561pubmed: 16277781google scholar: lookup
  25. Murray J-A M D, Longland A C, Moore-Colyer M J S, Dunnett C. The effect of feeding a low- or high-starch diet on the in vitro fermentative capacity of equine faecal inocula.. Anim. Sci. 2006 82:627–635.
    doi: 10.1079/ASC200683google scholar: lookup
  26. Murray J M D, McMullin P, Handel I, Hastie P M. Comparison of intestinal contents from different regions of the equine gastrointestinal tract as inocula for use in an in vitro gas production technique.. Anim. Feed Sci. Technol. 2014 187:98–103.
  27. Murray J-A M D, Scott B, Hastie P M. Fermentative capacity of equine Faecal inocula obtained from clinically normal horses and those predisposed to laminitis.. Anim. Feed Sci. Technol. 2009 151:306–311.
    doi: 10.1016/j.anifeedsci.2009.01.011google scholar: lookup
  28. NRC. Nutrient requirements of horses 6th ed. 2007 Washington (DC): National Academies Press.
  29. Reeves M J, Salman M D, Smith G. Risk factor for equine acute abdominal disease (colic): results from a multi-centered case-control study.. Prev. Vet. Med. 1996 26:285.
    doi: 10.1016/0167-5877(95)00551-Xgoogle scholar: lookup
  30. Ribeiro J, Gaspar S, Pinho M, Freirem J P B, Falcao-e-Cunha L. Sodium butyrate in growing and fattening diets for early-weaned rabbits.. W. Rab. Sci. 2012 20:199–207.
    doi: 10.4995/wrs.2012.1233google scholar: lookup
  31. Soltani M, Kazemi-Bonchenari M, Khaltabaki-Farahani A H, Afsarian O. Interaction of forage provision (alfalfa hay) and sodium butyrate supplementation on performance, structural growth, blood metabolites and rumen fermentation characteristics of lambs during pre-weaning period.. Anim. Feed Sci. Technol. 2017 230:77–86.
    doi: 10.1016/j.anifeedsci.2017.06.002google scholar: lookup
  32. Theodorou M K, Williams B A, Dhanoa M S, McAllan A B, France J. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds.. Anim. Feed Sci. Technol. 1994 48:185–197.
    doi: 10.1016/0377-8401(94)90171-6google scholar: lookup
  33. Wambacq W A, van Doorn D A, Rovers-Paap P M, Ducatelle R, Vlaminck L, Lourenço M, Hesta M. Dietary supplementation of coated butyrate in healthy horses: effect on gut histology and immunohistochemistry parameters.. 2017 In: Proceedings of the 21st Congress European Society of Veterinary & Comparative Nutrition.Cirencester, United Kingdom; p. 158.
  34. Wang J F, Chen Y X, Wang Z X, Dong S H, Lai Z W. Effects of sodium butyrate on structure of small intestine mucous epithelium in weaning piglets.. Chin. J. Vet. Sci. Technol. 2005 35:298–301.
  35. Warren L K, Kivipelto J. Comparison of cecal or faecal inocula used in the determination of in vitro fibre digestibility.. 2005 In: Proceedings of the 19th Equine Science Society Symposium, Tuscon, AZ, p. 304.
  36. White N A. Epidemiology and etiology of colic. The equine acute abdomen.. 1990 Malvern (PA): Lea & Febiger; p. 50–64.
  37. Williams B A, Bosch M W, Boer H, Verstegen M W A, Tamminga S. An in vitro batch culture method to assess potential fermentability of feed ingredients for monogastric diets.. Anim. Feed Sci. Technol. 2005 123–124:445–462.
    doi: 10.1016/j.anifeedsci.2005.04.031google scholar: lookup
  38. Yang H-J, Tamminga S, Williams B A, Dijkstra J, Boer H. In vitro gas and volatile fatty acids production profiles of barley and maize and their soluble and washout fractions after feed processing.. Anim. Feed Sci. Technol. 2005 120:125–140.
    doi: 10.1016/j.anifeedsci.2005.01.007google scholar: lookup
  39. Zhou D, Pan Q, Xin FZ, Zhang RN, He CX, Chen GY, Liu C, Chen YW, Fan JG. Sodium butyrate attenuates high-fat diet-induced steatohepatitis in mice by improving gut microbiota and gastrointestinal barrier.. World J Gastroenterol 2017 Jan 7;23(1):60-75.
    doi: 10.3748/wjg.v23.i1.60pmc: PMC5221287pubmed: 28104981google scholar: lookup

Citations

This article has been cited 1 times.
  1. Kara K, Altınsoy A. Comparison of forages’ digestion levels for different in vitro digestion techniques in horses. Vet Med Sci 2024 Mar;10(2):e31373.
    doi: 10.1002/vms3.1373pubmed: 38369823google scholar: lookup
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