Evaluation of key miRNAs during early pregnancy in Kazakh horse using RNA sequencing.
Abstract: miRNA has an important role in cell differentiation, biological development, and physiology. Milk production is an important quantitative trait in livestock and miRNA plays a role in the amount of milk produced. Methods: The role of regulatory miRNAs involved in equine milk production is not fully understood. We constructed two miRNA libraries for Kazakh horse milk production from higher-producing (H group) and lower-producing (L group) individuals, and used RNA-Seq technology to identify the differentially expressed miRNAs between the two milk phenotypes of Kazakh horses. Results: A total of 341 known and 333 novel miRNAs were detected from the H and L groups, respectively. Eighty-three differentially expressed miRNAs were identified between the H and L group s, of which 32 were known miRNAs (27 were up-regulated, five were down-regulated) and 51 were novel miRNAs (nine were up-regulated, 42 were down-regulated). A total of 2,415 genes were identified. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that these genes were annotated to mammary gland development, mammary gland morphogenesis, tissue development and PI3K-Akt signaling pathways, insulin signaling pathway and TGF-beta signaling pathway, among others. Five miRNAs (miR-199a-3p, miR143, miR145, miR221, miR486-5p) were identified as affecting horse milk production and these five miRNAs were validated using qRT-PCR. Conclusions: We described a methodology for the transcriptome-wide profiling of miRNAs in milk, which may help the design of new intervention strategies to improve the milk yield of Kazakh horses.
©2021 Liu et al.
Publication Date: 2021-02-23 PubMed ID: 33665012PubMed Central: PMC7908884DOI: 10.7717/peerj.10796Google Scholar: Lookup
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Summary
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This research investigated the roles of various miRNAs in milk production in Kazakh horses, discovering some that are differentially expressed in higher vs. lower milk-yielding horse populations, and suggesting possibilities for improving milk yield through miRNA intervention.
Objective and Methodology
- The goal of this research was to investigate the function of regulatory miRNAs in the milk production of Kazakh horses. Understanding this could shed light on quantitative traits like milk yield in livestock.
- The researchers developed two miRNA libraries from Kazakh horses with high milk production (H group) and low milk production (L group).
- The sequencing technology, RNA-Seq, was used to reveal differentially expressed miRNAs between the two groups.
Results
- In the high and low milk production groups, a total of 341 known and 333 novel miRNAs were detected respectively.
- There was differential expression of 83 miRNAs between the two groups, with 32 identified as known (27 up-regulated, 5 down-regulated) and 51 as novel miRNAs (9 up-regulated, 42 down-regulated).
- Through this process, the researchers were able to identify 2,415 genes.
- Both Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses suggested that these genes were associated with mammary gland development and morphogenesis, tissue development, and several signaling pathways.
- Five miRNAs (miR-199a-3p, miR143, miR145, miR221, miR486-5p) were found to have potential influence on horse milk production. These miRNAs were further validated using a method known as qRT-PCR.
Conclusions
- This study offers a methodology for profiling miRNAs on a transcriptome-wide scale within milk, which could guide new intervention strategies aimed to enhance milk yield in Kazakh horses.
- The research also adds to the existing body of knowledge on the role of miRNAs in milk production, which is an important quantitative trait in livestock.
Cite This Article
APA
Liu L, Fang C, Sun Y, Liu W.
(2021).
Evaluation of key miRNAs during early pregnancy in Kazakh horse using RNA sequencing.
PeerJ, 9, e10796.
https://doi.org/10.7717/peerj.10796 Publication
Researcher Affiliations
- College of Animal Science, Xinjiang Agriculture University, Urumqi, Xinjiang, China.
- Department of Animal Production, Farah Research Centre from the Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
- College of Animal Science, Xinjiang Agriculture University, Urumqi, Xinjiang, China.
- College of Animal Science, Xinjiang Agriculture University, Urumqi, Xinjiang, China.
Conflict of Interest Statement
The authors declare there are no competing interests.
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Citations
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