Evaluation of peritoneal fluid pH, glucose concentration, and lactate dehydrogenase activity for detection of septic peritonitis in horses.
Abstract: To determine whether peritoneal fluid pH, glucose concentration, and lactate dehydrogenase activity can be used to differentiate horses with septic peritonitis from those with nonseptic peritonitis. Methods: Prospective study. Methods: 46 horses, including 10 healthy horses, 15 horses with septic peritonitis, and 21 horses with nonseptic peritonitis. Methods: Peritoneal fluid and blood samples were analyzed for pH, glucose concentration, and lactate dehydrogenase activity. Complete blood cell counts were performed, and peritoneal fluid samples were submitted for bacterial culture. Results: Horses with septic peritonitis had significantly lower peritoneal fluid pH and glucose concentrations than horses with nonseptic peritonitis and healthy horses. Compared with other tests, serum-to-peritoneal fluid glucose concentration differences > 50 mg/dl had the highest diagnostic use for detection of septic peritonitis. Peritoneal fluid pH < 7.3, glucose concentration 200 mg/dl were also highly indicative of septic peritonitis. Conclusions: Peritoneal fluid pH and glucose concentration can be used to assist in the identification of horses with septic peritonitis. These measurements can provide an early indication of sepsis, especially if cytologic evaluation of peritoneal fluid is unavailable or results are equivocal and peritoneal fluid bacterial culture results are pending.
Publication Date: 1999-04-14 PubMed ID: 10200799
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This scientific research determined that the pH level and glucose concentration in peritoneal fluid can help in identifying horses suffering from septic peritonitis, providing an early warning sign of the condition especially where results of cytologic evaluation or bacterial culture are pending or inconclusive.
Objective of the Research
- The main objective of this study was to ascertain whether pH levels and glucose concentration in peritoneal fluid, and lactate dehydrogenase activity, can be used as indicators for differentiating horses with septic peritonitis from those with nonseptic peritonitis.
Research Methodology
- The study was carried out on 46 horses which were further classified into three groups: 10 healthy horses, 15 horses with septic peritonitis, and 21 horses with nonseptic peritonitis.
- For each horse, a peritoneal fluid and a blood sample were analyzed for parameters such as pH, glucose concentration, and lactate dehydrogenase activity. Furthermore, the peritoneal fluid samples were also subjected to bacterial culture.
Research Findings
- The outcomes showed that horses suffering from septic peritonitis exhibited considerably lower peritoneal fluid pH and glucose concentrations, compared to healthy horses and those with nonseptic peritonitis.
- Of all the tests conducted, the largest diagnostic use, for identifying septic peritonitis, was found to be attributed to serum-to-peritoneal fluid glucose concentration differences greater than 50 mg/dl.
- Additional indicators of septic peritonitis were identified as peritoneal fluid pH levels below 7.3, glucose concentrations under 30 mg/dl, and fibrinogen concentrations higher than 200 mg/dl.
Conclusions from the Research
- The study concluded that peritoneal fluid pH and glucose concentration can aid the identification process in horses afflicted with septic peritonitis.
- These tests can serve as early detectors of sepsis, even in situations where peritoneal fluid bacterial culture results are not available or are indecisive.
Cite This Article
APA
Van Hoogmoed L, Rodger LD, Spier SJ, Gardner IA, Yarbrough TB, Snyder JR.
(1999).
Evaluation of peritoneal fluid pH, glucose concentration, and lactate dehydrogenase activity for detection of septic peritonitis in horses.
J Am Vet Med Assoc, 214(7), 1032-1036.
Publication
Researcher Affiliations
- Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis 95616, USA.
MeSH Terms
- Animals
- Ascitic Fluid / chemistry
- Ascitic Fluid / enzymology
- Ascitic Fluid / microbiology
- Ascitic Fluid / veterinary
- Blood Cell Count / veterinary
- Blood Glucose / analysis
- Fibrinogen / analysis
- Glucose / analysis
- Horse Diseases / diagnosis
- Horse Diseases / microbiology
- Horses
- Hydrogen-Ion Concentration
- L-Lactate Dehydrogenase / analysis
- L-Lactate Dehydrogenase / blood
- Peritonitis / diagnosis
- Peritonitis / microbiology
- Peritonitis / veterinary
- Prospective Studies
Citations
This article has been cited 3 times.- Klein R, Nagy O, Tóthová C, Chovanová F. Clinical and Diagnostic Significance of Lactate Dehydrogenase and Its Isoenzymes in Animals. Vet Med Int 2020;2020:5346483.
- Cao S, Liu P, Zhu H, Gong H, Yao J, Sun Y, Geng G, Wang T, Feng S, Han M, Zhou J, Xu Y. Extracellular Acidification Acts as a Key Modulator of Neutrophil Apoptosis and Functions. PLoS One 2015;10(9):e0137221.
- Lohmann KL, Lewis SR, Wobeser B, Allen AL. Penetrating metallic foreign bodies as a cause of peritonitis in 3 horses. Can Vet J 2010 Dec;51(12):1400-4.
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