Evaluation of serum amyloid A protein as an acute-phase reactive protein in horses.
Abstract: Serum amyloid A protein (SAA) was isolated from equine acute-phase serum by repeating Sephadex G-75 gel filtration 3 times. Quantitative measurement of equine SAA was performed by the single radial immunodiffusion technique with rabbit anti-equine SAA serum. In clinically normal horses, the SAA concentration remained relatively high from immediately after birth up to 1 week of age. After this the concentration showed periodic fluctiation in the range of approximately 13 to 30 micrograms/ml. The mean (+/- SD) concentration of SAA in foals ( or = 18 months old) was 19.37 +/- 9.41 and 21.53 +/- 9.81 micrograms/ml, respectively. In mares during the perinatal period, the SAA concentration remained stable and within the normal range for 4 months before parturition. After foaling, it increased quickly and reached a peak value of 136.78 +/- 56.74 micrograms/ml on day 3 postpartum, and then began to decrease at 2 weeks postpartum returning to within the normal range by 1 month postpartum. In horses with experimentally induced inflammation, the SAA concentration increased quickly, and reached the highest value, approximately 4 to 20 times higher than pre-treatment values, on day 2 after treatment. It then returned to the base line values within 10 days to 4 weeks, concurrent with the disappearance of local inflammatory signs. The SAA concentration was very high in most horses with clinical signs of inflammation. It was concluded from these data that equine SAA was a sensitive acute-phase reactive protein which increased in the early phase of various acute inflammations.
Publication Date: 1993-12-01 PubMed ID: 7509640DOI: 10.1292/jvms.55.1011Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates Serum Amyloid A protein (SAA) as an acute-phase reactive protein in horses. It includes studies on how its concentration changes in newborn horses, in adult horses, in perinatal mares, and in horses with induced inflammation.
Research Method
- Serum Amyloid A protein (SAA) was isolated from equine acute-phase serum by repeating Sephadex G-75 gel filtration three times.
- The quantitative measurement of equine SAA was performed using the single radial immunodiffusion technique with rabbit anti-equine SAA serum.
Findings in Clinically Normal Horses
- SAA concentration in horses was relatively high after birth up to one week old. Post this period, the concentration showed periodic fluctuation in the range of approximately 13 to 30 micrograms/ml.
- The average concentration of SAA in foals and adult horses was found close, with 19.37 +/- 9.41 and 21.53 +/- 9.81 micrograms/ml, respectively.
Findings in Perinatal Mares
- In mares during the perinatal period, the SAA concentration remained stable and in the normal range for 4 months before giving birth.
- After foaling, SAA concentration increased quickly and reached peak value of 136.78 +/- 56.74 micrograms/ml on day 3 postpartum, and then began to decrease at 2 weeks postpartum returning to the normal range by 1 month postpartum.
Findings in Horses with Induced Inflammation
- In horses with experimentally induced inflammation, the SAA concentration increased quickly. It reached the highest value, approximately 4 to 20 times higher than pre-treatment values, on day 2 post treatment. It then returned to the base line values within 10 days to 4 weeks, concurrent with the disappearance of local inflammatory signs.
- The SAA concentration was very high in most horses with clinical signs of inflammation.
Conclusion
- Based on the collected data, the research concluded that equine SAA is a sensitive acute-phase reactive protein. Its concentration increases in the initial phase of various acute inflammations, making it an effective protein to keep track of inflammatory conditions in horses.
Cite This Article
APA
Nunokawa Y, Fujinaga T, Taira T, Okumura M, Yamashita K, Tsunoda N, Hagio M.
(1993).
Evaluation of serum amyloid A protein as an acute-phase reactive protein in horses.
J Vet Med Sci, 55(6), 1011-1016.
https://doi.org/10.1292/jvms.55.1011 Publication
Researcher Affiliations
- Department of Veterinary Surgery, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
MeSH Terms
- Acute-Phase Proteins / analysis
- Acute-Phase Proteins / biosynthesis
- Aging / blood
- Animals
- Animals, Newborn
- Female
- Horse Diseases
- Horses / blood
- Immunodiffusion
- Inflammation / blood
- Inflammation / veterinary
- Jejunostomy / veterinary
- Male
- Orchiectomy
- Ovariectomy
- Pregnancy
- Pregnancy, Animal / blood
- Reference Values
- Serum Amyloid A Protein / analysis
- Serum Amyloid A Protein / biosynthesis
Citations
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