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Evaluation of serum concentration of acute-phase proteins (haptoglobin and serum amyloid A) in the affected Arabian foals with rhodococcosis.
Abstract: Early detection of Rhodococcus equi pneumonia in foals is essential for horse health and for veterinarians. Objective: This study aimed to demonstrate the usefulness of assessing the serum concentration of acute-phase proteins (APPs) in the early diagnosis of pneumonia. Methods: The study evaluated APPs in 19 Arabian foals with R. equi pneumonia and compared them with 18 normal Arabian foals in equestrian clubs in Tabriz, Iran. Affected foals were identified through history, clinical findings and bacterial culture of tracheal washing. Biochemical methods and polymerase chain reaction tests were performed by examining the 16S rRNA and vapA genes to confirm the diagnosis of bacterial isolates. Blood samples were taken from all sick and healthy horses, and their serum was isolated. APPs in the serum were measured in all the samples. Results: Rhodococcosis increased the serum concentration of haptoglobin (Hp) and serum amyloid A (SAA) (p < 0.001). The relationship between SAA and Hp was meaningful in the infected group (r = 0.933) but not in the healthy group. In cases where there are clinical findings of R. equi pneumonia, the concentration of SAA and Hp can help the effectiveness of treatment. Unassigned: Serum concentration analysis of APPs can be helpful in early diagnosis and successfully treating foals with R. equi pneumonia.
© 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.
Publication Date: 2022-11-24 PubMed ID: 36423204PubMed Central: PMC9856977DOI: 10.1002/vms3.1005Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article investigates the utility of measuring the concentration of certain proteins in the blood for the early detection of a type of pneumonia in Arabian foals. The results reveal that an increase in these proteins, known as acute-phase proteins, can signal an infection in the early stages.
Research Objectives and Methods
This study was designed to evaluate the potential of acute-phase proteins (APPs) in detecting Rhodococcus equi pneumonia in Arabian foals at an early stage. The APPs of interest were haptoglobin (Hp) and serum amyloid A (SAA). These proteins are usually present in higher concentrations in the blood during inflammation or infection.
- The researchers studied 19 Arabian foals suffering from R. equi pneumonia and contrasted their results with those from 18 healthy foals. All the foals belonged to equestrian clubs located in Tabriz, Iran.
- The sick foals were selected based on their medical history, clinical symptoms, and the presence of the R. equi bacteria in their tracheal washing as confirmed by bacterial culture.
- To confirm R. equi infection, biochemical tests and polymerase chain reaction methods targeting 16S rRNA and vapA genes were employed.
- After identifying the sick and healthy foals, the researchers collected blood samples from each one, from which they extracted serum for APP measurement.
Research Findings
The main findings from the research are:
- Rhodococcus equi pneumonia resulted in a significant increase in the serum concentration of haptoglobin and serum amyloid A in the infected foals as compared to the healthy ones.
- There was a significant correlation between the levels of SAA and Hp in the infected group, which was absent in the healthy group.
- In cases where clinical symptoms of R. equi pneumonia were present, the concentration of SAA and Hp can be beneficial in determining the efficacy of the treatment.
Implications of the Findings
The researchers concluded that the serum concentration analysis of acute-phase proteins, specifically haptoglobin and serum amyloid A, could assist in the early diagnosis of Rhodococcus equi pneumonia. This early detection can ensure that treatment can begin sooner, thereby improving the prognosis for infected foals. This finding is relevant for the care and management of Arabian foals in equestrian settings.
Cite This Article
APA
Hassanpour A, Moghaddam S.
(2022).
Evaluation of serum concentration of acute-phase proteins (haptoglobin and serum amyloid A) in the affected Arabian foals with rhodococcosis.
Vet Med Sci, 9(1), 144-149.
https://doi.org/10.1002/vms3.1005 Publication
Researcher Affiliations
- Department of Clinical Science, Tabriz Medical Sciences Branch, Islamic Azad University, Tabriz, Iran.
- Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
MeSH Terms
- Animals
- Horses
- Serum Amyloid A Protein / analysis
- Serum Amyloid A Protein / metabolism
- Acute-Phase Proteins
- Haptoglobins
- Actinomycetales Infections / diagnosis
- Actinomycetales Infections / microbiology
- Actinomycetales Infections / veterinary
- RNA, Ribosomal, 16S
- Pneumonia, Bacterial / diagnosis
- Pneumonia, Bacterial / microbiology
- Pneumonia, Bacterial / veterinary
- Horse Diseases / diagnosis
- Horse Diseases / microbiology
Conflict of Interest Statement
The authors declare that there is no conflict of interest.
References
This article includes 29 references
- Belgrave RL, Dickey MM, Arheart KL, Cray C. Assessment of serum amyloid A testing of horses and its clinical application in a specialized equine practice. Journal of the American Veterinary Medical Association 243(1), 113–119.
- Berg LC, Thomsen PD, Andersen PH, Jensen HE, Jacobsen S. Serum amyloid A is expressed in histologically normal tissues from horses and cattle. Veterinary Immunology and Immunopathology 144(1‐2), 155–159.
- Browning L, Krebs J, Jebb S. Discrimination ratio analysis of inflammatory markers: implications for the study of inflammation in chronic disease. Metabolism 53(7), 899–903.
- Carter A, Martin N. Serum sialic acid levels in health and disease. Journal of Clinical Pathology 15(1), 69–72.
- Chaffin MK, Cohen ND, Martens RJ, Edwards RF, Nevill M. Foal‐related risk factors associated with development of Rhodococcus equi pneumonia on farms with endemic infection. Journal of the American Veterinary Medical Association 223(12), 1791–1799.
- Chavan MM, Kawle PD, Mehta NG. Increased sialylation and defucosylation of plasma proteins are early events in the acute phase response. Glycobiology 15(9), 838–848.
- Cohen ND. Treating foals with Rhodococcus equi infection: What do you recommend?. Compendium on Continuing Education for the Practising Veterinarian 1(1), 14–18.
- Cohen ND, Chaffin M, Vandenplas M, Edwards R, Nevill M, Moore J, Martens R. Study of serum amyloid A concentrations as a means of achieving early diagnosis of Rhodococcus equi pneumonia. Equine Veterinary Journal 37(3), 212–216.
- Constable PD, Hinchcliff KW, Done SH, Grünberg W. Veterinary medicine: A textbook of the diseases of cattle, horses, sheep, pigs and goats (11th ed.). .
- Cray C, Belgrave RL. Haptoglobin quantitation in serum samples from clinically normal and clinically abnormal horses. Journal of Equine Veterinary Science 34(2), 337–340.
- Cray C, Zaias J, Altman NH. Acute phase response in animals: A review. Comparative Medicine 59(6), 517–526.
- De Cozar M, Sherlock C, Knowles E, Mair T. Serum amyloid A and plasma fibrinogen concentrations in horses following emergency exploratory celiotomy. Equine Veterinary Journal 52(1), 59–66.
- Eckersall P. Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Revue de Medecine Veterinaire 151(7), 577–584.
- Giguère S, Berghaus L, Miller C. Clinical assessment of a point‐of‐care serum amyloid A assay in foals with bronchopneumonia. Journal of Veterinary Internal Medicine 30(4), 1338–1343.
- Hultén C, Demmers S. Serum amyloid A (SAA) as an aid in the management of infectious disease in the foal: Comparison with total leucocyte count, neutrophil count and fibrinogen. Equine Veterinary Journal 34(7), 693–698.
- Hultén C, Grönlund U, Hirvonen J, Tulamo RM, Suominen M, Marhaug G, Forsberg M. Dynamics in serum of the inflammatory markers serum amyloid A (SAA), haptoglobin, fibrinogen and α2‐globulins during induced noninfectious arthritis in the horse. Equine Veterinary Journal 34(7), 699–704.
- Hultén C, Sandgren B, Skiöldebrand E, Klingeborn B, Marhaug G, Forsberg M. The acute phase protein serum amyloid A (SAA) as an inflammatory marker in equine influenza virus infection. Acta Veterinaria Scandinavica 40(4), 323–333.
- Hultén C, Tulamo R‐M, Suominen M, Burvall K, Marhaug G, Forsberg M. A non‐competitive chemiluminescence enzyme immunoassay for the equine acute phase protein serum amyloid A (SAA)–a clinically useful inflammatory marker in the horse. Veterinary Immunology and Immunopathology 68(2‐4), 267–281.
- Kent JE, Goodall J. Assessment of an immunoturbidimetric method for measuring equine serum haptoglobin concentrations. Equine Veterinary Journal 23(1), 59–66.
- Letek M, Ocampo‐Sosa AA, Sanders M, Fogarty U, Buckley T, Leadon DP, González P, Scortti M, Meijer WG, Parkhill J, Bentley S, Vázquez‐Boland JA. Evolution of the Rhodococcus equi vap pathogenicity island seen through comparison of host‐associated vapA and vapB virulence plasmids. Journal of Bacteriology 190(17), 5797–5805.
- Muscatello G, Leadon D, Klay M, Ocampo‐Sosa A, Lewis D, Fogarty U, Buckley T, Gilkerson JR, Meijer WG, Vazquez‐Boland J. Rhodococcus equi infection in foals: The science of ‘rattles’. Equine Veterinary Journal 39(5), 470–478.
- Passamonti F, Vardi D, Stefanetti V, Marenzoni M, Prato S, Cévese P, Coletti M, Pepe M, Casagrande Proietti P, Olea‐Popelka F. Rhodococcus equi pneumonia in foals: An assessment of the early diagnostic value of serum amyloid A and plasma fibrinogen concentrations in equine clinical practice. The Veterinary Journal 203(2), 211–218.
- Petersen HH, Ersbøll AK, Jensen CS, Nielsen JP. Serum‐haptoglobin concentration in Danish slaughter pigs of different health status. Preventive Veterinary Medicine 54(4), 325–335.
- Pihl TH, Andersen PH, Kjelgaard‐Hansen M, Mørck NB, Jacobsen S. Serum amyloid A and haptoglobin concentrations in serum and peritoneal fluid of healthy horses and horses with acute abdominal pain. Veterinary Clinical Pathology 42(2), 177–183.
- Reed SM, Bayly WM, Sellon DC. Equine internal medicine. .
- Satué K, Calvo A, Gardón JC. Factors influencing serum amyloid type A (SAA) concentrations in horses. Open Journal of Veterinary Medicine 3(01), 58.
- Sellon DC, Besser TE, Vivrette SL, McConnico RS. Comparison of nucleic acid amplification, serology, and microbiologic culture for diagnosis of Rhodococcus equi pneumonia in foals. Journal of Clinical Microbiology 39(4), 1289–1293.
- Skinner J, Brown R, Roberts L. Bovine haptoglobin response in clinically defined field conditions. The Veterinary Record 128(7), 147–149.
- Skinner J, Roberts L. Haptoglobin as an indicator of infection in sheep. The Veterinary Record 134(2), 33–36.
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