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Serum amyloid A is expressed in histologically normal tissues from horses and cattle.
Abstract: mRNA expression of the acute phase protein serum amyloid A (SAA) in histologically normal tissues derived from horses (n=13) and cattle (n=4) was investigated by quantitative reverse-transcriptase real-time polymerase-chain reaction. As expected, high constitutive SAA mRNA expression was demonstrated in hepatic tissue in both species. In horses, moderate (>1% of the hepatic expression) SAA mRNA expression was detected in the lung, mammary gland, pancreas, synovial membrane, thymus, thyroid gland and uterus. Other equine tissues and organs sampled included adipose tissue, adrenal gland, aorta, brain, different gastro-intestinal tissues, heart, kidney, lymph node, ovary, testis, prostate, skeletal and cardiac muscle, skin and spleen; all showed low (<1% of the hepatic expression) SAA mRNA expression. In cattle, SAA mRNA was expressed in moderate levels in adipose tissue, colon, jejunum, mammary gland, skeletal muscle, synovial membrane, thymus, thyroid gland, and uterus; expression was low in the remainder of the samples (same tissue panel as horses). The results confirm the liver as the main site of SAA production. Even though there was some inter-species variation in tissues expressing SAA mRNA, several organs communicating with the external environment (lung, mammary gland, uterus, and certain parts of the gastro-intestinal tract) showed SAA mRNA expression, which supports the hypothesis that SAA might possess a role in the innate defence against invading pathogens. The results of the study thus warrant further studies into functions of hepatically and extrahepatically produced SAA isoforms.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication Date: 2011-07-05 PubMed ID: 21783263DOI: 10.1016/j.vetimm.2011.06.037Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article explores the expression of the acute phase protein serum amyloid A (SAA) in normal tissues derived from cattle and horses. It further confirms the liver as the main site for SAA production and suggests that SAA could potentially play a role in defending against invading pathogens.
Experimental Design & Methodology
- The study focused on mRNA expression of serum amyloid A (SAA), an acute phase protein, in healthy tissue samples derived from horses (n=13) and cattle (n=4).
- The method used for the investigation was quantitative reverse-transcriptase real-time polymerase chain reaction, a technique commonly used in molecular biology to detect and quantify a specific DNA sequence in a DNA sample.
Key Findings
- The study confirmed high constitutive SAA mRNA expression in hepatic (liver) tissue in both species, emphasizing the liver as the main site for SAA production.
- In horses, moderate SAA mRNA expression (>1% of the hepatic expression) was found in multiple areas such as lung, mammary gland, pancreas, synovial membrane, thymus, thyroid gland, and uterus. All other sampled tissues showed low (<1% of hepatic expression) levels of SAA mRNA expression.
- In cattle, moderate levels of SAA mRNA expression were found in adipose tissue, colon, jejunum, mammary gland, skeletal muscle, synovial membrane, thymus, thyroid gland, and uterus. The remaining tissues displayed low SAA mRNA expression levels.
Significance and Implications
- The results highlight potential variations in the tissues expressing SAA mRNA between different species.
- Several organs that interact with the external environment – such as the lungs, mammary glands, uterus, and certain parts of the gastrointestinal tract – showed SAA mRNA expression. This finding supports the hypothesis that SAA might play a key role in innate defense mechanisms against invading pathogens.
- The findings of the study underline the need for further research into the functions of SAA isoforms produced both within and outside the liver (hepatically and extrahepatically), to better understand the physiological role of SAA and its potential implications in health and disease.
Cite This Article
APA
Berg LC, Thomsen PD, Andersen PH, Jensen HE, Jacobsen S.
(2011).
Serum amyloid A is expressed in histologically normal tissues from horses and cattle.
Vet Immunol Immunopathol, 144(1-2), 155-159.
https://doi.org/10.1016/j.vetimm.2011.06.037 Publication
Researcher Affiliations
- Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Gronnegaardsvej 7, 1870 Frederiksberg C, Denmark.
MeSH Terms
- Animals
- Cattle / immunology
- Cattle / metabolism
- Female
- Horses / immunology
- Horses / metabolism
- Male
- Real-Time Polymerase Chain Reaction / veterinary
- Serum Amyloid A Protein / analysis
- Serum Amyloid A Protein / metabolism
- Tissue Distribution
Citations
This article has been cited 16 times.- Shinya U, Yamato O, Iwamura Y, Kato T, Hamada Y, Widodo OS, Taniguchi M, Takagi M. Usefulness of Measuring Serum Amyloid A Concentration in Japanese Black Cattle in Clinical Practice.. Vet Sci 2023 Aug 17;10(8).
- Palmisano M, Javsicas L, McNaughten J, Gamsju00e4ger L, Renaud DL, Gomez DE. Effect of plasma transfusion on serum amyloid A concentration in healthy neonatal foals and foals with failure of transfer of passive immunity.. J Vet Intern Med 2023 Mar;37(2):697-702.
- Hassanpour A, Moghaddam S. Evaluation of serum concentration of acute-phase proteins (haptoglobin and serum amyloid A) in the affected Arabian foals with rhodococcosis.. Vet Med Sci 2023 Jan;9(1):144-149.
- Widodo OS, Nishihara S, Pambudi D, Kusakabe KT, Taura Y, Nishi Y, Yamato O, Taniguchi M, Takagi M. Relationship Between Ovary Size and Anti-Mu00fcllerian Hormone Levels in Holstein-Friesian Cows.. Front Vet Sci 2022;9:828123.
- Widodo OS, Etoh M, Kokushi E, Uno S, Yamato O, Pambudi D, Okawa H, Taniguchi M, Lamid M, Takagi M. Practical Application of Urinary Zearalenone Monitoring System for Feed Hygiene Management of a Japanese Black Cattle Breeding Herd-The Relationship between Monthly Anti-Mu00fcllerian Hormone and Serum Amyloid A Concentrations.. Toxins (Basel) 2022 Feb 16;14(2).
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