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Home / Equine Research Bank / Am J Vet Res / Evaluation of the osteogenic and chondrogenic differentiatio...
American journal of veterinary research2010; 71(10); 1228-1236; doi: 10.2460/ajvr.71.10.1228

Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells.

Abstract: To evaluate the proliferative behavior, telomere length, immunophenotype, and differentiation capacity of equine adipose tissue-derived mesenchymal stem cells (AT-MSCs). Methods: 6 adult racing horses treated for articular Injury but otherwise healthy. Methods: AT-MSCs were Isolated from horses and expanded In Dulbecco modified Eagle medium enriched with fetal bovine serum and antimicrobials. Expression of cell surface antigens and telomere length were Investigated via flow cytometry Differentiation of MSCs Into chondrocytes, osteoblasts, and adipocytes was Induced In vitro by specific stimuli and was evaluated by analyzing marker genes with quantitative reverse transcriptase PCR assays and immunocytochemical and cytologie evaluations. Results: Equine MSCs could be cultured up to the fifth passage before signs of senescence, apoptosis, and detachment Indicated cellular exhaustion. However, the AT-MSCs from 2 of 6 horses survived to later passages with Increased doubling rates and telomere lengths. The cells had a typical phenotype, with expression of CD14, CD73, CD90, CD105, CD140b, and CD164 antigens and a lack of CD34 and CD45 antigens. The cells also had a strong potential to differentiate Into osteoblasts, as characterized by Intense von Kossa and alizarin red staining as well as high Induction of osteopontin. Chondrogenic differentiation was detected via Alelan blue staining and expression of aggrecan and type II collagen Adipogenesis was Induced in AT-MSCs by supplementation of differentiation media with rabbit serum. Conclusions: Equine AT-MSCs representa suitable cellular source for regenerative treatment of bone or cartilage defects, particularly when expanded In vitro for only a few passages.
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Publication Date: 2010-10-06 PubMed ID: 20919912DOI: 10.2460/ajvr.71.10.1228Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the growth behavior, telomere length, immunophenotype, and differentiation capacity of mesenchymal stem cells (AT-MSCs) derived from horse adipose tissue, which could potentially be used in the regenerative treatment for bone or cartilage defects in horses.

Research Methods

  • The study involved 6 adult racing horses, which were treated for articular injury but were otherwise healthy.
  • The AT-MSCs were isolated from the horses and expanded in a Dulbecco modified Eagle medium enriched with fetal bovine serum and antimicrobials.
  • Through flow cytometry, the researchers analyzed the expression of cell surface antigens and the length of the cells’ telomeres.
  • The researchers induced the differentiation of MSCs into chondrocytes (cells that produce and maintain the cartilaginous matrix), osteoblasts (bone-forming cells), and adipocytes (fat cells) in vitro, using specific stimuli and analyzing marker genes with quantitative reverse transcriptase PCR assays as well as immunocytochemical and cytologic evaluations.

Findings

  • It was found that equine MSCs could be cultured up to the fifth passage before signs of senescence, apoptosis, and detachment indicated cellular exhaustion.
  • However, the AT-MSCs from 2 of the 6 horses survived to later passages, showing increased doubling rates and longer telomeres.
  • The cells displayed a typical phenotype with expression of CD14, CD73, CD90, CD105, CD140b, and CD164 antigens, and no expression of CD34 and CD45 antigens.
  • The cells had strong potential to differentiate into osteoblasts, identified by intense Von Kossa and alizarin red staining, as well as high induction of osteopontin, a protein important in bone formation.
  • Chondrogenic differentiation was identified through Alcian Blue staining and expression of aggrecan and type II collagen, both of which are important components of cartilage.
  • Adipogenesis was induced in AT-MSCs through supplementation of the differentiation media with rabbit serum.

Conclusion

  • The research concludes that equine AT-MSCs could be a suitable cellular source for regenerative treatment of bone or cartilage defects, especially when expanded in vitro for only a few passages.

Cite This Article

APA
Braun J, Hack A, Weis-Klemm M, Conrad S, Treml S, Kohler K, Walliser U, Skutella T, Aicher WK. (2010). Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells. Am J Vet Res, 71(10), 1228-1236. https://doi.org/10.2460/ajvr.71.10.1228

Publication

American journal of veterinary research
ISSN: 0002-9645
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 71
Issue: 10
Pages: 1228-1236

Researcher Affiliations

Braun, Julian
  • Center for Regenerative Medicine, University Hospital Tübingen, D-72076 Tübingen, Germany.
Hack, Anita
    Weis-Klemm, Michaela
      Conrad, Sabine
        Treml, Sabrina
          Kohler, Konrad
            Walliser, Ulrich
              Skutella, Thomas
                Aicher, Wilhelm K

                  MeSH Terms

                  • Adipose Tissue / cytology
                  • Animals
                  • Antigens, CD / genetics
                  • Antigens, CD / metabolism
                  • Bone and Bones / cytology
                  • Cartilage / cytology
                  • Cartilage / physiology
                  • Cell Differentiation / physiology
                  • Cell Proliferation
                  • Cells, Cultured
                  • Gene Expression Regulation / physiology
                  • Horses
                  • Immunophenotyping
                  • Mesenchymal Stem Cells / cytology
                  • Mesenchymal Stem Cells / physiology
                  • Osteogenesis / physiology
                  • Telomere

                  Citations

                  This article has been cited 32 times.
                  1. Petrova V, Yonkova P, Simeonova G, Vachkova E. Horse serum potentiates cellular viability and improves indomethacin-induced adipogenesis in equine subcutaneous adipose-derived stem cells (ASCs).. Int J Vet Sci Med 2023;11(1):94-105.
                    doi: 10.1080/23144599.2023.2248805pubmed: 37655053google scholar: lookup
                  2. Stage HJ, Trappe S, Söllig K, Trachsel DS, Kirsch K, Zieger C, Merle R, Aschenbach JR, Gehlen H. Multilineage Differentiation Potential of Equine Adipose-Derived Stromal/Stem Cells from Different Sources.. Animals (Basel) 2023 Apr 15;13(8).
                    doi: 10.3390/ani13081352pubmed: 37106915google scholar: lookup
                  3. Trachsel DS, Stage HJ, Rausch S, Trappe S, Söllig K, Sponder G, Merle R, Aschenbach JR, Gehlen H. Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine.. Animals (Basel) 2022 Aug 11;12(16).
                    doi: 10.3390/ani12162049pubmed: 36009640google scholar: lookup
                  4. Song K, Yang GM, Han J, Gil M, Dayem AA, Kim K, Lim KM, Kang GH, Kim S, Jang SB, Vellingiri B, Cho SG. Modulation of Osteogenic Differentiation of Adipose-Derived Stromal Cells by Co-Treatment with 3, 4'-Dihydroxyflavone, U0126, and N-Acetyl Cysteine.. Int J Stem Cells 2022 Aug 30;15(3):334-345.
                    doi: 10.15283/ijsc22044pubmed: 35769058google scholar: lookup
                  5. Hagen A, Lehmann H, Aurich S, Bauer N, Melzer M, Moellerberndt J, Patané V, Schnabel CL, Burk J. Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture.. Front Bioeng Biotechnol 2020;8:613621.
                    doi: 10.3389/fbioe.2020.613621pubmed: 33553119google scholar: lookup
                  6. Kannan S, Ghosh J, Dhara SK. Osteogenic differentiation potential of porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media.. Biol Open 2020 Oct 19;9(10).
                    doi: 10.1242/bio.053280pubmed: 32973080google scholar: lookup
                  7. Al Naem M, Bourebaba L, Kucharczyk K, Röcken M, Marycz K. Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders.. Stem Cell Rev Rep 2020 Apr;16(2):301-322.
                    doi: 10.1007/s12015-019-09932-0pubmed: 31797146google scholar: lookup
                  8. Contentin R, Demoor M, Concari M, Desancé M, Audigié F, Branly T, Galéra P. Comparison of the Chondrogenic Potential of Mesenchymal Stem Cells Derived from Bone Marrow and Umbilical Cord Blood Intended for Cartilage Tissue Engineering.. Stem Cell Rev Rep 2020 Feb;16(1):126-143.
                    doi: 10.1007/s12015-019-09914-2pubmed: 31745710google scholar: lookup
                  9. Merlo B, Teti G, Lanci A, Burk J, Mazzotti E, Falconi M, Iacono E. Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells.. BMC Vet Res 2019 Aug 2;15(1):277.
                    doi: 10.1186/s12917-019-2023-5pubmed: 31375144google scholar: lookup
                  10. Saadeldin IM, Swelum AA, Noreldin AE, Tukur HA, Abdelazim AM, Abomughaid MM, Alowaimer AN. Isolation and Culture of Skin-Derived Differentiated and Stem-Like Cells Obtained from the Arabian Camel (Camelus dromedarius).. Animals (Basel) 2019 Jun 20;9(6).
                    doi: 10.3390/ani9060378pubmed: 31226810google scholar: lookup
                  11. Liu J, Lu Y, Liu J, Jin C, Meng Y, Pei D. Influence of epigallocatechin-3-gallate in promoting proliferation and osteogenic differentiation of human periodontal ligament cells.. BMC Oral Health 2019 May 2;19(1):73.
                    doi: 10.1186/s12903-019-0768-7pubmed: 31046751google scholar: lookup
                  12. Yamasaki A, Omura T, Murata D, Kobayashi M, Sunaga T, Kusano K, Ueno Y, Kuramoto T, Hobo S, Misumi K. A pilot study of regenerative therapy by implanting synovium-derived mesenchymal stromal cells in equine osteochondral defect models.. J Equine Sci 2018 Dec;29(4):117-122.
                    doi: 10.1294/jes.29.117pubmed: 30607136google scholar: lookup
                  13. Desancé M, Contentin R, Bertoni L, Gomez-Leduc T, Branly T, Jacquet S, Betsch JM, Batho A, Legendre F, Audigié F, Galéra P, Demoor M. Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy.. Int J Mol Sci 2018 Feb 10;19(2).
                    doi: 10.3390/ijms19020537pubmed: 29439436google scholar: lookup
                  14. Ishikawa S, Horinouchi C, Mizoguchi R, Senokuchi A, Kamikakimoto R, Murata D, Hatazoe T, Tozaki T, Misumi K, Hobo S. Isolation of equine peripheral blood stem cells from a Japanese native horse.. J Equine Sci 2017;28(4):153-158.
                    doi: 10.1294/jes.28.153pubmed: 29270073google scholar: lookup
                  15. Tidd N, Michelsen J, Hilbert B, Quinn JC. Minicircle Mediated Gene Delivery to Canine and Equine Mesenchymal Stem Cells.. Int J Mol Sci 2017 Apr 12;18(4).
                    doi: 10.3390/ijms18040819pubmed: 28417917google scholar: lookup
                  16. Tillotson M, Logan N, Brett P. Osteogenic stem cell selection for repair and regeneration.. Bone Rep 2016 Dec;5:22-32.
                    doi: 10.1016/j.bonr.2016.01.003pubmed: 28326344google scholar: lookup
                  17. Zayed M, Caniglia C, Misk N, Dhar MS. Donor-Matched Comparison of Chondrogenic Potential of Equine Bone Marrow- and Synovial Fluid-Derived Mesenchymal Stem Cells: Implications for Cartilage Tissue Regeneration.. Front Vet Sci 2016;3:121.
                    doi: 10.3389/fvets.2016.00121pubmed: 28149840google scholar: lookup
                  18. Ishikawa S, Horinouchi C, Murata D, Matsuzaki S, Misumi K, Iwamoto Y, Korosue K, Hobo S. Isolation and characterization of equine dental pulp stem cells derived from Thoroughbred wolf teeth.. J Vet Med Sci 2017 Jan 20;79(1):47-51.
                    doi: 10.1292/jvms.16-0131pubmed: 27818457google scholar: lookup
                  19. Metcalf GL, McClure SR, Hostetter JM, Martinez RF, Wang C. Evaluation of adipose-derived stromal vascular fraction from the lateral tailhead, inguinal region, and mesentery of horses.. Can J Vet Res 2016 Oct;80(4):294-301.
                    pubmed: 27733784
                  20. Song X, Hong C, Zheng Q, Zhao H, Song K, Liu Z, Shen J, Li Y, Wang J, Shen T. Differentiation potential of rabbit CD90-positive cells sorted from adipose-derived stem cells in vitro.. In Vitro Cell Dev Biol Anim 2017 Jan;53(1):77-82.
                    doi: 10.1007/s11626-016-0081-6pubmed: 27553254google scholar: lookup
                  21. Shikh Alsook MK, Gabriel A, Piret J, Waroux O, Tonus C, Connan D, Baise E, Antoine N. Tissues from equine cadaver ligaments up to 72 hours of post-mortem: a promising reservoir of stem cells.. Stem Cell Res Ther 2015 Dec 18;6:253.
                    doi: 10.1186/s13287-015-0250-7pubmed: 26684484google scholar: lookup
                  22. Tessier L, Bienzle D, Williams LB, Koch TG. Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.. PLoS One 2015;10(4):e0122954.
                    doi: 10.1371/journal.pone.0122954pubmed: 25902064google scholar: lookup
                  23. Radtke CL, Nino-Fong R, Rodriguez-Lecompte JC, Esparza Gonzalez BP, Stryhn H, McD￮ LA. Osteogenic potential of sorted equine mesenchymal stem cell subpopulations.. Can J Vet Res 2015 Apr;79(2):101-8.
                    pubmed: 25852225
                  24. Mohanty N, Gulati BR, Kumar R, Gera S, Kumar S, Kumar P, Yadav PS. Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood.. Cytotechnology 2016 Aug;68(4):795-807.
                    doi: 10.1007/s10616-014-9831-zpubmed: 25487085google scholar: lookup
                  25. Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential.. Stem Cell Res Ther 2014 Feb 21;5(1):25.
                    doi: 10.1186/scrt414pubmed: 24559797google scholar: lookup
                  26. Mohanty N, Gulati BR, Kumar R, Gera S, Kumar P, Somasundaram RK, Kumar S. Immunophenotypic characterization and tenogenic differentiation of mesenchymal stromal cells isolated from equine umbilical cord blood.. In Vitro Cell Dev Biol Anim 2014 Jun;50(6):538-48.
                    doi: 10.1007/s11626-013-9729-7pubmed: 24414976google scholar: lookup
                  27. Carrade DD, Borjesson DL. Immunomodulation by mesenchymal stem cells in veterinary species.. Comp Med 2013 Jun;63(3):207-17.
                    pubmed: 23759523
                  28. Mohammadi-Sangcheshmeh A, Shafiee A, Seyedjafari E, Dinarvand P, Toghdory A, Bagherizadeh I, Schellander K, Cinar MU, Soleimani M. Isolation, characterization, and mesodermic differentiation of stem cells from adipose tissue of camel (Camelus dromedarius).. In Vitro Cell Dev Biol Anim 2013 Feb;49(2):147-54.
                    doi: 10.1007/s11626-012-9578-9pubmed: 23299319google scholar: lookup
                  29. Carrade DD, Lame MW, Kent MS, Clark KC, Walker NJ, Borjesson DL. Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().. Cell Med 2012;4(1):1-11.
                    doi: 10.3727/215517912X647217pubmed: 23152950google scholar: lookup
                  30. Ranera B, Remacha AR, Álvarez-Arguedas S, Romero A, Vázquez FJ, Zaragoza P, Martín-Burriel I, Rodellar C. Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue.. BMC Vet Res 2012 Aug 22;8:142.
                    doi: 10.1186/1746-6148-8-142pubmed: 22913590google scholar: lookup
                  31. Requicha JF, Viegas CA, Albuquerque CM, Azevedo JM, Reis RL, Gomes ME. Effect of anatomical origin and cell passage number on the stemness and osteogenic differentiation potential of canine adipose-derived stem cells.. Stem Cell Rev Rep 2012 Dec;8(4):1211-22.
                    doi: 10.1007/s12015-012-9397-0pubmed: 22773405google scholar: lookup
                  32. Mensing N, Gasse H, Hambruch N, Haeger JD, Pfarrer C, Staszyk C. Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse.. BMC Vet Res 2011 Aug 2;7:42.
                    doi: 10.1186/1746-6148-7-42pubmed: 21810270google scholar: lookup
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