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Vaccines2020; 8(2); 268; doi: 10.3390/vaccines8020268

Evaluation of Three Different Vaccination Protocols against EHV1/EHV4 Infection in Mares: Double Blind, Randomized Clinical Trial.

Abstract: EHV1 and EHV4 are the most important herpesviruses in horses. Repeated cases of abortion in mares regularly vaccinated, prompted us to investigate the immune response after vaccination with the same inactivated vaccine, but with three different protocols. Eighteen mares were chosen and randomly divided in three study groups (G-G-G) and a control group (Ctrl). For serologic and PCR investigations nasal swabs, sera and blood were collected. The protocol used in G (4 doses) increased the titer recorded by ELISA and seroneutralization (SN). Poor agreement and no correlation were observed in titer values between ELISA and SN and between SN and PCR. A very weak positive correlation between ELISA and PCR was obtained. Seven out of 18 nasal swabs were positive by PCR; none showed viremia and no abortion occurred, regardless of vaccination status and despite active circulation of EHV-1 in the farm at the time of the study. The study was conducted in field conditions, in a susceptible population with a known history of infection and abortion, and among the three protocols, the one proposed in the G was the least efficient while the one proposed for the G, seems to have induced a higher antibody titer in both SN and ELISA.
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Publication Date: 2020-06-01 PubMed ID: 32492841PubMed Central: PMC7350013DOI: 10.3390/vaccines8020268Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents a clinical trial study that tested three different vaccination protocols aimed at protecting horses against EHV1 and EHV4 herpesviruses. The study illustrates the varying levels of efficiency of the protocols in producing immune response in mares.

Introduction and Methodology

  • The study was motivated by frequent cases of abortion in mares that were already regularly vaccinated against EHV1 and EHV4, leading the researchers to explore the immune response after vaccination with the same inactivated vaccine, but under different protocols.
  • A total of eighteen mares were selected and randomly segregated into three study groups (G-G-G) and a control group (Ctrl). The chest groups were each subjected to a different vaccination protocol.
  • Data for the study was collected through nasal swabs, blood samples, and sera for serological and Polymerase Chain Reaction (PCR) investigations.

Findings and Implications

  • The protocol followed by one of the study groups, the G (4 doses) group, was noted to have increased the antibody titer as identified via Enzyme-Linked Immunosorbent Assay (ELISA) and Seroneutralization (SN).
  • The study revealed poor agreement and lack of correlation in titer values between ELISA and SN, as well as between SN and PCR.
  • Only a very weak positive correlation was found between ELISA and PCR.
  • PCR analysis on nasal swabs revealed that seven out of the eighteen samples were positive; however, none showed signs of viremia (presence of viruses in the blood) and no abortion occurred, irrespective of the vaccination status. This was despite active circulation of EHV-1 in the farm during the study period.
  • Among the three protocols tested in real-world conditions, the method proposed for one of the study groups proved to be the least efficient. On the other hand, the protocol followed by another group appeared to have induced a higher antibody titer in both SN and ELISA tests.
  • The findings of the study shed light on the effectiveness of different vaccination protocols and call attention to the need for further analysis and improvements in the existing horse vaccination protocols against herpesviruses.

Cite This Article

APA
(2020). Evaluation of Three Different Vaccination Protocols against EHV1/EHV4 Infection in Mares: Double Blind, Randomized Clinical Trial. Vaccines (Basel), 8(2), 268. https://doi.org/10.3390/vaccines8020268

Publication

Vaccines
ISSN: 2076-393X
NlmUniqueID: 101629355
Country: Switzerland
Language: English
Volume: 8
Issue: 2
PII: 268

Researcher Affiliations

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 30 references
  1. Fortier G, van Erck E, Pronost S, Lekeux P, Thiry E. Equine gammaherpesviruses: pathogenesis, epidemiology and diagnosis.. Vet J 2010 Nov;186(2):148-56.
    doi: 10.1016/j.tvjl.2009.08.017pubmed: 19766026google scholar: lookup
  2. Davison AJ. Herpesvirus systematics.. Vet Microbiol 2010 Jun 16;143(1):52-69.
    doi: 10.1016/j.vetmic.2010.02.014pmc: PMC2995426pubmed: 20346601google scholar: lookup
  3. Patel JR, Heldens J. Equine herpesviruses 1 (EHV-1) and 4 (EHV-4)--epidemiology, disease and immunoprophylaxis: a brief review.. Vet J 2005 Jul;170(1):14-23.
    doi: 10.1016/j.tvjl.2004.04.018pubmed: 15993786google scholar: lookup
  4. Kolb AW, Lewin AC, Moeller Trane R, McLellan GJ, Brandt CR. Phylogenetic and recombination analysis of the herpesvirus genus varicellovirus.. BMC Genomics 2017 Nov 21;18(1):887.
    doi: 10.1186/s12864-017-4283-4pmc: PMC5697016pubmed: 29157201google scholar: lookup
  5. Telford EA, Watson MS, McBride K, Davison AJ. The DNA sequence of equine herpesvirus-1.. Virology 1992 Jul;189(1):304-16.
    doi: 10.1016/0042-6822(92)90706-Upubmed: 1318606google scholar: lookup
  6. Telford EA, Watson MS, Perry J, Cullinane AA, Davison AJ. The DNA sequence of equine herpesvirus-4.. J Gen Virol 1998 May;79 ( Pt 5):1197-203.
    doi: 10.1099/0022-1317-79-5-1197pubmed: 9603335google scholar: lookup
  7. Crabb BS, Studdert MJ. Comparative studies of the proteins of equine herpesviruses 4 and 1 and asinine herpesvirus 3: antibody response of the natural hosts.. J Gen Virol 1990 Sep;71 ( Pt 9):2033-41.
    doi: 10.1099/0022-1317-71-9-2033pubmed: 2170572google scholar: lookup
  8. Crabb BS, Allen GP, Studdert MJ. Characterization of the major glycoproteins of equine herpesviruses 4 and 1 and asinine herpesvirus 3 using monoclonal antibodies.. J Gen Virol 1991 Sep;72 ( Pt 9):2075-82.
    doi: 10.1099/0022-1317-72-9-2075pubmed: 1716650google scholar: lookup
  9. Allen GP. Respiratory infections by Equine Herpesvirus types 1 and 4. .
  10. Harless W, Pusterla N. Equine Herpesvirus 1 and 4 respiratory disease in the horse. Clin. Tech. Equine Pract. 2006;5:197–202.
    doi: 10.1053/j.ctep.2006.03.014google scholar: lookup
  11. Van de Walle GR, Goupil R, Wishon C, Damiani A, Perkins GA, Osterrieder N. A single-nucleotide polymorphism in a herpesvirus DNA polymerase is sufficient to cause lethal neurological disease.. J Infect Dis 2009 Jul 1;200(1):20-5.
    doi: 10.1086/599316pubmed: 19456260google scholar: lookup
  12. Pusterla N, David Wilson W, Madigan JE, Ferraro GL. Equine herpesvirus-1 myeloencephalopathy: a review of recent developments.. Vet J 2009 Jun;180(3):279-89.
    doi: 10.1016/j.tvjl.2008.08.004pubmed: 18805030google scholar: lookup
  13. Kydd JH, Townsend HG, Hannant D. The equine immune response to equine herpesvirus-1: the virus and its vaccines.. Vet Immunol Immunopathol 2006 May 15;111(1-2):15-30.
    doi: 10.1016/j.vetimm.2006.01.005pubmed: 16476492google scholar: lookup
  14. O'Neill T, Kydd JH, Allen GP, Wattrang E, Mumford JA, Hannant D. Determination of equid herpesvirus 1-specific, CD8+, cytotoxic T lymphocyte precursor frequencies in ponies.. Vet Immunol Immunopathol 1999 Sep 1;70(1-2):43-54.
    doi: 10.1016/S0165-2427(99)00037-9pubmed: 10507286google scholar: lookup
  15. Goehring LS, Wagner B, Bigbie R, Hussey SB, Rao S, Morley PS, Lunn DP. Control of EHV-1 viremia and nasal shedding by commercial vaccines.. Vaccine 2010 Jul 19;28(32):5203-11.
    doi: 10.1016/j.vaccine.2010.05.065pubmed: 20538091google scholar: lookup
  16. Goodman LB, Wagner B, Flaminio MJ, Sussman KH, Metzger SM, Holland R, Osterrieder N. Comparison of the efficacy of inactivated combination and modified-live virus vaccines against challenge infection with neuropathogenic equine herpesvirus type 1 (EHV-1).. Vaccine 2006 Apr 24;24(17):3636-45.
    doi: 10.1016/j.vaccine.2006.01.062pubmed: 16513225google scholar: lookup
  17. Heldens JG, Hannant D, Cullinane AA, Prendergast MJ, Mumford JA, Nelly M, Kydd JH, Weststrate MW, van den Hoven R. Clinical and virological evaluation of the efficacy of an inactivated EHV1 and EHV4 whole virus vaccine (Duvaxyn EHV1,4). Vaccination/challenge experiments in foals and pregnant mares.. Vaccine 2001 Jul 20;19(30):4307-17.
    doi: 10.1016/S0264-410X(01)00131-1pubmed: 11457558google scholar: lookup
  18. Bresgen C, Lämmer M, Wagner B, Osterrieder N, Damiani AM. Serological responses and clinical outcome after vaccination of mares and foals with equine herpesvirus type 1 and 4 (EHV-1 and EHV-4) vaccines.. Vet Microbiol 2012 Nov 9;160(1-2):9-16.
    doi: 10.1016/j.vetmic.2012.04.042pubmed: 22633483google scholar: lookup
  19. Foote CE, Love DN, Gilkerson JR, Whalley JM. Serological responses of mares and weanlings following vaccination with an inactivated whole virus equine herpesvirus 1 and equine herpesvirus 4 vaccine.. Vet Microbiol 2002 Aug 2;88(1):13-25.
    doi: 10.1016/S0378-1135(02)00100-1pubmed: 12119135google scholar: lookup
  20. Foote CE, Love DN, Gilkerson JR, Wellington JE, Whalley JM. EHV-1 and EHV-4 infection in vaccinated mares and their foals.. Vet Immunol Immunopathol 2006 May 15;111(1-2):41-6.
    doi: 10.1016/j.vetimm.2006.01.007pubmed: 16513181google scholar: lookup
  21. Crabb BS, Studdert MJ. Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host.. J Virol 1993 Oct;67(10):6332-8.
    doi: 10.1128/JVI.67.10.6332-6338.1993pmc: PMC238063pubmed: 7690425google scholar: lookup
  22. . Equine Rhinopneumonitis (Infection with Equid Herpesvirus-1 and -4). OIE Manual for Terrestrial Animals 2008. Chapter 3, 5.9.
  23. Wang L, Raidal SL, Pizzirani A, Wilcox GE. Detection of respiratory herpesviruses in foals and adult horses determined by nested multiplex PCR.. Vet Microbiol 2007 Mar 31;121(1-2):18-28.
    doi: 10.1016/j.vetmic.2006.11.009pubmed: 17208393google scholar: lookup
  24. Bannai H, Mae N, Ode H, Nemoto M, Tsujimura K, Yamanaka T, Kondo T, Matsumura T. Successful control of winter pyrexias caused by equine herpesvirus type 1 in Japanese training centers by achieving high vaccination coverage.. Clin Vaccine Immunol 2014 Aug;21(8):1070-6.
    doi: 10.1128/CVI.00258-14pmc: PMC4135906pubmed: 24872513google scholar: lookup
  25. Dunowska M, Gopakumar G, Perrott MR, Kendall AT, Waropastrakul S, Hartley CA, Carslake HB. Virological and serological investigation of Equid herpesvirus 1 infection in New Zealand.. Vet Microbiol 2015 Apr 17;176(3-4):219-28.
    doi: 10.1016/j.vetmic.2015.01.016pubmed: 25666453google scholar: lookup
  26. Crabb BS, MacPherson CM, Reubel GH, Browning GF, Studdert MJ, Drummer HE. A type-specific serological test to distinguish antibodies to equine herpesviruses 4 and 1.. Arch Virol 1995;140(2):245-58.
    doi: 10.1007/BF01309860pubmed: 7710353google scholar: lookup
  27. Mumford EL, Traub-Dargatz JL, Carman J, Callan RJ, Collins JK, Goltz KL, Romm SR, Tarr SF, Salman MD. Occurrence of infectious upper respiratory tract disease and response to vaccination in horses on six sentinel premises in northern Colorado.. Equine Vet J 2003 Jan;35(1):72-7.
    doi: 10.2746/042516403775467379pubmed: 12553466google scholar: lookup
  28. Yasunaga S, Maeda K, Matsumura T, Kondo T, Kai K. Application of a type-specific enzyme-linked immunosorbent assay for equine herpesvirus types 1 and 4 (EHV-1 and -4) to horse populations inoculated with inactivated EHV-1 vaccine.. J Vet Med Sci 2000 Jul;62(7):687-91.
    doi: 10.1292/jvms.62.687pubmed: 10945284google scholar: lookup
  29. Lunn DP, Davis-Poynter N, Flaminio MJ, Horohov DW, Osterrieder K, Pusterla N, Townsend HG. Equine herpesvirus-1 consensus statement.. J Vet Intern Med 2009 May-Jun;23(3):450-61.
    doi: 10.1111/j.1939-1676.2009.0304.xpubmed: 19645832google scholar: lookup
  30. Pusterla N, Chaney KP, Maes R, Wise AG, Holland R, Schott HC 2nd. Investigation of the molecular detection of vaccine-derived equine herpesvirus type 1 in blood and nasal secretions from horses following intramuscular vaccination.. J Vet Diagn Invest 2007 May;19(3):290-3.
    doi: 10.1177/104063870701900311pubmed: 17459860google scholar: lookup

Citations

This article has been cited 4 times.
  1. Petrini S, Maple P. Herpesvirus Vaccines. Vaccines (Basel) 2022 Apr 18;10(4).
    doi: 10.3390/vaccines10040628pubmed: 35455377google scholar: lookup
  2. El Brini Z, Fassi Fihri O, Paillot R, Lotfi C, Amraoui F, El Ouadi H, Dehhaoui M, Colitti B, Alyakine H, Piro M. Seroprevalence of Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) in the Northern Moroccan Horse Populations. Animals (Basel) 2021 Sep 29;11(10).
    doi: 10.3390/ani11102851pubmed: 34679874google scholar: lookup
  3. Warda FF, Ahmed HES, Shafik NG, Mikhael CA, Abd-ElAziz HMG, Mohammed WA, Shosha EA. Application of equine herpesvirus-1 vaccine inactivated by both formaldehyde and binary ethylenimine in equine. Vet World 2021 Jul;14(7):1815-1821.
    doi: 10.14202/vetworld.2021.1815-1821pubmed: 34475703google scholar: lookup
  4. Kutumbetov L, Myrzakhmetova B, Tussipova A, Zhapparova G, Tlenchiyeva T, Bissenbayeva K, Nurabayev S, Kerimbayev A. Development and Preclinical Evaluation of a Lyophilized Vaccine Against Equine Herpesvirus Type 4 (EHV-4). Vaccines (Basel) 2025 May 31;13(6).
    doi: 10.3390/vaccines13060604pubmed: 40573935google scholar: lookup
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