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Home / Equine Research Bank / Influenza Other Respir Viruses / Evaluation of twenty-two rapid antigen detection tests in th...
Influenza and other respiratory viruses2016; 10(2); 127-133; doi: 10.1111/irv.12358

Evaluation of twenty-two rapid antigen detection tests in the diagnosis of Equine Influenza caused by viruses of H3N8 subtype.

Abstract: Equine influenza (EI) is a highly contagious disease caused by viruses of the H3N8 subtype. The rapid diagnosis of EI is essential to reduce the disease spread. Many rapid antigen detection (RAD) tests for diagnosing human influenza are available, but their ability to diagnose EI has not been systematically evaluated. Objective: The aim of this study was to compare the performance of 22 RAD tests in the diagnosis of EI. Methods: The 22 RAD tests were performed on fivefold serial dilutions of EI virus to determine their detection limits. The four most sensitive RAD tests (ImmunoAce Flu, BD Flu examan, Quick chaser Flu A, B and ESPLINE Influenza A&B-N) were further evaluated using nasopharyngeal samples collected from experimentally infected and naturally infected horses. The results were compared to those obtained using molecular tests. Results: The detection limits of the 22 RAD tests varied hugely. Even the four RAD tests showing the best sensitivity were 125-fold less sensitive than the molecular techniques. The duration of virus detection in the experimentally infected horses was shorter using the RAD tests than using the molecular techniques. The RAD tests detected between 27% and 73% of real-time RT-PCR-positive samples from naturally infected horses. Conclusions: The study demonstrated the importance of choosing the right RAD tests as only three of 22 were fit for diagnosing EI. It was also indicated that even RAD tests with the highest sensitivity serve only as an adjunct to molecular tests because of the potential for false-negative results.
© 2015 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
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Publication Date: 2016-02-01 PubMed ID: 26568369PubMed Central: PMC4746556DOI: 10.1111/irv.12358Google Scholar: Lookup
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  • Comparative Study
  • Evaluation Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper sought to determine the reliability of 22 rapid antigen detection (RAD) tests in diagnosing Equine Influenza (EI), a communicable ailment incited by H3N8 subtype viruses. Accuracy was evaluated against the standard of molecular testing techniques, with results indicating even the most sensitive RAD test was significantly less reliable.

Research Objective and Methods

  • The study aimed to gauge the dependability of 22 RAD tests for diagnosing EI compared to molecular tests. This was done to help curtail the spread of EI with quick, accurate diagnoses.
  • The 22 RAD tests were executed on progressively diluted EI virus to establish their detection thresholds.
  • Following that, they specifically studied the four most sensitive RAD tests (ImmunoAce Flu, BD Flu examan, Quick chaser Flu A, B and ESPLINE Influenza A&B-N) on nasopharyngeal samples harvested from both experimentally and naturally infected equines.
  • The results of these tests were then scrutinized in relation to those procured through molecular examinations.

Research Results

  • Data indicated a wide disparity in the detection limits of the 22 RAD tests. Even the four RAD tests with the best sensitivity proved to be 125-fold less reliable than the molecular techniques.
  • The RAD tests demonstrated a shorter virus detection duration in experimentally infected horses compared to molecular methods, signifying a high likelihood of false negatives.
  • The RADs detection performance with naturally infected horses varied substantially too, picking up between 27% and 73% of samples rendered positive through real-time RT-PCR.

Conclusion of the Study

  • The examination underscored the necessity of selecting an appropriate RAD test for diagnosing EI, as only three out of the 22 evaluated tests were deemed fit for purpose.
  • The findings also suggested that despite their expedience, RAD test results should only support, but not supplant molecular test results due to the potential for yielding false-negative outcomes.

Cite This Article

APA
Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Kondo T, Matsumura T, Gildea S, Cullinane A. (2016). Evaluation of twenty-two rapid antigen detection tests in the diagnosis of Equine Influenza caused by viruses of H3N8 subtype. Influenza Other Respir Viruses, 10(2), 127-133. https://doi.org/10.1111/irv.12358

Publication

Influenza and other respiratory viruses
ISSN: 1750-2659
NlmUniqueID: 101304007
Country: England
Language: English
Volume: 10
Issue: 2
Pages: 127-133

Researcher Affiliations

Yamanaka, Takashi
  • Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
Nemoto, Manabu
  • Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
Bannai, Hiroshi
  • Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
Tsujimura, Koji
  • Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
Kondo, Takashi
  • Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
Matsumura, Tomio
  • Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
Gildea, Sarah
  • Virology Unit, Irish Equine Centre, Johnstown, Naas, Co. Kildare, Ireland.
Cullinane, Ann
  • Virology Unit, Irish Equine Centre, Johnstown, Naas, Co. Kildare, Ireland.

MeSH Terms

  • Animals
  • Antibodies, Viral
  • Antigens, Viral / analysis
  • Enzyme-Linked Immunosorbent Assay
  • Horse Diseases / diagnosis
  • Horse Diseases / virology
  • Horses
  • Humans
  • Influenza A Virus, H3N8 Subtype / genetics
  • Influenza A Virus, H3N8 Subtype / immunology
  • Influenza A Virus, H3N8 Subtype / isolation & purification
  • Influenza, Human / diagnosis
  • Influenza, Human / virology
  • Japan
  • Nasopharynx / virology
  • Orthomyxoviridae Infections / diagnosis
  • Orthomyxoviridae Infections / veterinary
  • Orthomyxoviridae Infections / virology
  • RNA, Viral
  • Reagent Kits, Diagnostic
  • Real-Time Polymerase Chain Reaction / standards
  • Sensitivity and Specificity

References

This article includes 29 references
  1. Wilson WD. Equine influenza.. Vet Clin North Am Equine Pract 1993 Aug;9(2):257-82.
    pubmed: 8358645doi: 10.1016/s0749-0739(17)30395-4google scholar: lookup
  2. van Maanen C, Cullinane A. Equine influenza virus infections: an update.. Vet Q 2002 Jun;24(2):79-94.
    pubmed: 12095083doi: 10.1080/01652176.2002.9695127google scholar: lookup
  3. Webster RG, Bean WJ, Gorman OT, Chambers TM, Kawaoka Y. Evolution and ecology of influenza A viruses.. Microbiol Rev 1992 Mar;56(1):152-79.
    pmc: PMC372859pubmed: 1579108doi: 10.1128/mr.56.1.152-179.1992google scholar: lookup
  4. Webster RG. Are equine 1 influenza viruses still present in horses?. Equine Vet J 1993 Nov;25(6):537-8.
    pubmed: 8276003doi: 10.1111/j.2042-3306.1993.tb03009.xgoogle scholar: lookup
  5. Cullinane A, Newton JR. Equine influenza--a global perspective.. Vet Microbiol 2013 Nov 29;167(1-2):205-14.
    pubmed: 23680107doi: 10.1016/j.vetmic.2013.03.029google scholar: lookup
  6. Daly JM, MacRae S, Newton JR, Wattrang E, Elton DM. Equine influenza: a review of an unpredictable virus.. Vet J 2011 Jul;189(1):7-14.
    pubmed: 20685140doi: 10.1016/j.tvjl.2010.06.026google scholar: lookup
  7. Chambers TM, Reedy SE. Equine influenza culture methods.. Methods Mol Biol 2014;1161:403-10.
    pubmed: 24899449doi: 10.1007/978-1-4939-0758-8_35google scholar: lookup
  8. Quinlivan M, Dempsey E, Ryan F, Arkins S, Cullinane A. Real-time reverse transcription PCR for detection and quantitative analysis of equine influenza virus.. J Clin Microbiol 2005 Oct;43(10):5055-7.
    pmc: PMC1248463pubmed: 16207961doi: 10.1128/jcm.43.10.5055-5057.2005google scholar: lookup
  9. Balasuriya UB. Type A influenza virus detection from horses by real-time RT-PCR and insulated isothermal RT-PCR.. Methods Mol Biol 2014;1161:393-402.
    pubmed: 24899448doi: 10.1007/978-1-4939-0758-8_34google scholar: lookup
  10. Powell DG, Watkins KL, Li PH, Shortridge KF. Outbreak of equine influenza among horses in Hong Kong during 1992.. Vet Rec 1995 May 27;136(21):531-6.
    pubmed: 7660556doi: 10.1136/vr.136.21.531google scholar: lookup
  11. Wernery R, Yates PJ, Wernery U, Mumford JA. An equine influenza outbreak in a polo club in Dubai, United Arab Emirates in 1995/1996. in Wernery U, Wade JF, Mumford JA, Kaaden OR. (eds): Equine Infectious Diseases VIII. Newmarket: R&W Publications Ltd., 1999.
  12. van Maanen C, van Essen GJ, Minke J, Daly JM, Yates PJ. Diagnostic methods applied to analysis of an outbreak of equine influenza in a riding school in which vaccine failure occurred.. Vet Microbiol 2003 Jun 10;93(4):291-306.
    pubmed: 12713892doi: 10.1016/s0378-1135(03)00029-4google scholar: lookup
  13. McCabe VJ, Sindle T, Daly JM. Evaluation of the Binax NOW Flu A test kit for the rapid detection of equine influenza virus.. Vet Rec 2006 Feb 4;158(5):164-5.
    pubmed: 16461624doi: 10.1136/vr.158.5.164google scholar: lookup
  14. Chambers TM, Shortridge KF, Li PH, Powell DG, Watkins KL. Rapid diagnosis of equine influenza by the Directigen FLU-A enzyme immunoassay.. Vet Rec 1994 Sep 17;135(12):275-9.
    pubmed: 7817505doi: 10.1136/vr.135.12.275google scholar: lookup
  15. Yamanaka T, Tsujimura K, Kondo T, Matsumura T. Evaluation of antigen detection kits for diagnosis of equine influenza.. J Vet Med Sci 2008 Feb;70(2):189-92.
    pubmed: 18319581doi: 10.1292/jvms.70.189google scholar: lookup
  16. Chartrand C, Leeflang MM, Minion J, Brewer T, Pai M. Accuracy of rapid influenza diagnostic tests: a meta-analysis.. Ann Intern Med 2012 Apr 3;156(7):500-11.
    pubmed: 22371850doi: 10.7326/0003-4819-156-7-201204030-00403google scholar: lookup
  17. Yamanaka T, Niwa H, Tsujimura K, Kondo T, Matsumura T. Epidemic of equine influenza among vaccinated racehorses in Japan in 2007.. J Vet Med Sci 2008 Jun;70(6):623-5.
    pubmed: 18628606doi: 10.1292/jvms.70.623google scholar: lookup
  18. Sakai-Tagawa Y, Ozawa M, Tamura D, Le Mt, Nidom CA, Sugaya N, Kawaoka Y. Sensitivity of influenza rapid diagnostic tests to H5N1 and 2009 pandemic H1N1 viruses.. J Clin Microbiol 2010 Aug;48(8):2872-7.
    pmc: PMC2916590pubmed: 20554831doi: 10.1128/jcm.00439-10google scholar: lookup
  19. Yamanaka T, Bannai H, Nemoto M, Tsujimura K, Kondo T, Muranaka M, Hobo S, Minamijima YH, Yamada M, Matsumura T. Efficacy of a single intravenous dose of the neuraminidase inhibitor peramivir in the treatment of equine influenza.. Vet J 2012 Aug;193(2):358-62.
    pubmed: 22326934doi: 10.1016/j.tvjl.2012.01.005google scholar: lookup
  20. Galvin P, Gildea S, Nelly M, Quinlivan M, Arkins S, Walsh C, Cullinane A. The evaluation of three diagnostic tests for the detection of equine influenza nucleoprotein in nasal swabs.. Influenza Other Respir Viruses 2014 May;8(3):376-83.
    pmc: PMC4181487pubmed: 24512560doi: 10.1111/irv.12235google scholar: lookup
  21. Newton JR, Daly JM, Spencer L, Mumford JA. Description of the outbreak of equine influenza (H3N8) in the United Kingdom in 2003, during which recently vaccinated horses in Newmarket developed respiratory disease.. Vet Rec 2006 Feb 11;158(6):185-92.
    pubmed: 16474051doi: 10.1136/vr.158.6.185google scholar: lookup
  22. Nemoto M, Yamanaka T, Bannai H, Tsujimura K, Kondo T, Matsumura T. Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for H3N8 equine influenza virus.. J Virol Methods 2011 Dec;178(1-2):239-42.
    pubmed: 21907240doi: 10.1016/j.jviromet.2011.07.015google scholar: lookup
  23. Lu Z, Chambers TM, Boliar S, Branscum AJ, Sturgill TL, Timoney PJ, Reedy SE, Tudor LR, Dubovi EJ, Vickers ML, Sells S, Balasuriya UB. Development and evaluation of one-step TaqMan real-time reverse transcription-PCR assays targeting nucleoprotein, matrix, and hemagglutinin genes of equine influenza virus.. J Clin Microbiol 2009 Dec;47(12):3907-13.
    pmc: PMC2786649pubmed: 19846644doi: 10.1128/jcm.00598-09google scholar: lookup
  24. Nemoto M, Oue Y, Morita Y, Kanno T, Kinoshita Y, Niwa H, Ueno T, Katayama Y, Bannai H, Tsujimura K, Yamanaka T, Kondo T. Experimental inoculation of equine coronavirus into Japanese draft horses.. Arch Virol 2014 Dec;159(12):3329-34.
    pmc: PMC7087042pubmed: 25139547doi: 10.1007/s00705-014-2205-1google scholar: lookup
  25. Landis JR, Koch GG. The measurement of observer agreement for categorical data.. Biometrics 1977 Mar;33(1):159-74.
    pubmed: 843571
  26. Nishiura H, Satou K. Potential effectiveness of public health interventions during the equine influenza outbreak in racehorse facilities in Japan, 2007.. Transbound Emerg Dis 2010 Jun;57(3):162-70.
    pubmed: 20345573doi: 10.1111/j.1865-1682.2010.01134.xgoogle scholar: lookup
  27. Hurt AC, Baas C, Deng YM, Roberts S, Kelso A, Barr IG. Performance of influenza rapid point-of-care tests in the detection of swine lineage A(H1N1) influenza viruses.. Influenza Other Respir Viruses 2009 Jul;3(4):171-6.
    pmc: PMC4634687pubmed: 19627374doi: 10.1111/j.1750-2659.2009.00086.xgoogle scholar: lookup
  28. Guthrie AJ, Stevens KB, Bosman PP. The circumstances surrounding the outbreak and spread of equine influenza in South Africa.. Rev Sci Tech 1999 Apr;18(1):179-85.
    pubmed: 10190213doi: 10.20506/rst.18.1.1155google scholar: lookup
  29. Watson J, Daniels P, Kirkland P, Carroll A, Jeggo M. The 2007 outbreak of equine influenza in Australia: lessons learned for international trade in horses.. Rev Sci Tech 2011 Apr;30(1):87-93.
    pubmed: 21809755doi: 10.20506/rst.30.1.2021google scholar: lookup

Citations

This article has been cited 4 times.
  1. Chiba S. Effect of early oseltamivir on outpatients without hypoxia with suspected COVID-19. Wien Klin Wochenschr 2021 Apr;133(7-8):292-297.
    doi: 10.1007/s00508-020-01780-0pubmed: 33296027google scholar: lookup
  2. Singh RK, Dhama K, Karthik K, Khandia R, Munjal A, Khurana SK, Chakraborty S, Malik YS, Virmani N, Singh R, Tripathi BN, Munir M, van der Kolk JH. A Comprehensive Review on Equine Influenza Virus: Etiology, Epidemiology, Pathobiology, Advances in Developing Diagnostics, Vaccines, and Control Strategies. Front Microbiol 2018;9:1941.
    doi: 10.3389/fmicb.2018.01941pubmed: 30237788google scholar: lookup
  3. Yamanaka T, Nemoto M, Bannai H, Tsujimura K, Kondo T, Matsumura T, Fu TQH, Fernandez CJ, Gildea S, Cullinane A. Rapid diagnosis of equine influenza by highly sensitive silver amplification immunochromatography system. J Vet Med Sci 2017 Jun 16;79(6):1061-1063.
    doi: 10.1292/jvms.17-0105pubmed: 28458275google scholar: lookup
  4. Sandybayev N, Strochkov V, Beloussov V, Orkara S, Kydyrmanov A, Khan Y, Batanova Z, Kassenov M. Evaluation of a novel real-time polymerase chain reaction assay for identifying H3 equine influenza virus in Kazakhstan. Vet World 2023 Aug;16(8):1682-1689.
    doi: 10.14202/vetworld.2023.1682-1689pubmed: 37766711google scholar: lookup
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