Evaluation of ultrasmall superparamagnetic iron oxide contrast agent labeling of equine cord blood and bone marrow mesenchymal stromal cells.
Abstract: To evaluate the efficacy and effects of labeling equine umbilical cord blood (UCB)- and bone marrow (BM)-derived multipotent mesenchymal stromal cells (MSCs) with an ultrasmall superparamagnetic iron oxide (SPIO) contrast agent and the detection of labeled MSCs by use of MRI. Methods: UCB MSCs from placental tissues of 5 foals and BM MSCs from 5 horses. Methods: UCB and BM MSC cultures were seeded in duplicate (5,000 cells/cm(2)). One duplicate was incubated with SPIO (50 μg/mL); the other was processed identically, but without SPIO. Mesenchymal stromal cells were expanded in triplicates for 5 passages and assessed for viability and proliferative capacity, labeling efficacy, and labeled cell proportion. For MRI detection, 5 × 10(6) labeled BM MSCs from passage 1 or 2 were injected into a collagenase-induced superficial digital flexor tendon defect of an equine cadaveric forelimb from 2 horses. Results: For passages 1, 2, and 3, labeling efficacy and cell proportion for UCB MSCs (99.6% [range, 98.8% to 99.9%], 16.6% [range, 6.5% to 36.1%], and 1.0% [range, 0.4% to 2.8%], respectively) were significantly higher than for BM MSCs (99.2% [range, 97.8% to 99.7%], 4.5% [range, 1.6% to 11.8%], and 0.2% [range, 0.1% to 0.6%], respectively). Labeling was not detectable after passage 3. Viability of MSCs was not affected, but cell doubling time increased in labeled MSCs, compared with that of unlabeled MSCs. On MRI 3-D T2*-weighted fast gradient echo sequences, decreased signal intensity was observed for BM passage 1 MSCs. Conclusions: Equine UCB and BM MSCs were labeled with SPIO at high efficiencies.
Publication Date: 2014-10-29 PubMed ID: 25350092DOI: 10.2460/ajvr.75.11.1010Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Biochemistry
- Biotechnology
- Bone Marrow
- Cadaver Study
- Cell Proliferation
- Cell Viability
- Clinical Study
- Equine Diseases
- Equine Health
- Equine Science
- Experimental Methods
- Imaging Techniques
- In Vitro Research
- Magnetic Resonance Imaging
- Mesenchymal Cells
- Physiology
- Stem Cells
- Superficial Digital Flexor Tendon
- Veterinary Medicine
- Veterinary Research
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research paper focuses on the examination of the effectiveness and implications of labelling horse umbilical cord blood and bone marrow-derived stem cells with superparamagnetic iron oxide, a contrast agent, and tracking these labelled cells using MRI.
Objective and Methods of the Study
- The main aim of the study was to determine the success and impact of using ultrasmall superparamagnetic iron oxide (SPIO) to label mesenchymal stromal cells (MSCs) sourced from equine Umbilical Cord Blood (UCB) and Bone Marrow (BM).
- Furthermore, the study aimed to detect the labeled MSCs using MRI technology.
- UCB MSCs were collected from the placental tissues of five foals (young horses) while BM MSCs were collected from five adult horses.
- The collected cells were cultured in a controlled environment to stimulate growth.
- In each culture, some cells were treated with SPIO while others were left untreated to serve as a comparison.
- Mesenchymal stromal cells were expanded for five passages and measured for viability, proliferative capacity, labeling efficacy, and labeled cell proportion.
- For MRI detection, labeled BM MSCs were injected into an induced injury in a horse’s forelimb and then detected via MRI imaging.
Results of the Study
- Results indicated that the labeling efficacy and cell proportion for UCB MSCs was significantly higher than those from bone marrow.
- It was also found that this labeling was not detectable after the third passage, indicating that it may lose effectiveness over time.
- The study found no significant impact on the viability of the MSCs following the labeling, but it was observed that the cell doubling time increased in the labeled MSCs compared to the unlabeled ones.
- Through MRI imaging, a decreased signal intensity was observed for labeled bone marrow cells.
Conclusions from the Study
- Concluding the study, it was shown that equine UCB and BM MSCs could be effectively labeled with SPIO.
- The results of this study can be useful for subsequent research into tracking and understanding the behavior of MSCs in the body.
Cite This Article
APA
Bourzac CA, Koenig JB, Link KA, Nykamp SG, Koch TG.
(2014).
Evaluation of ultrasmall superparamagnetic iron oxide contrast agent labeling of equine cord blood and bone marrow mesenchymal stromal cells.
Am J Vet Res, 75(11), 1010-1017.
https://doi.org/10.2460/ajvr.75.11.1010 Publication
Researcher Affiliations
- Departments of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
MeSH Terms
- Animals
- Cadaver
- Contrast Media
- Dextrans
- Female
- Fetal Blood / cytology
- Forelimb
- Horses / anatomy & histology
- Horses / blood
- Magnetic Resonance Imaging
- Magnetite Nanoparticles
- Mesenchymal Stem Cells / cytology
- Placenta / cytology
- Pregnancy
- Serial Passage / veterinary
Citations
This article has been cited 8 times.- Lee DY, Lee SE, Kwon DH, Nithiyanandam S, Lee MH, Hwang JS, Basith S, Ahn JH, Shin TH, Lee G. Strategies to Improve the Quality and Freshness of Human Bone Marrow-Derived Mesenchymal Stem Cells for Neurological Diseases.. Stem Cells Int 2021;2021:8444599.
- Ahrberg AB, Horstmeier C, Berner D, Brehm W, Gittel C, Hillmann A, Josten C, Rossi G, Schubert S, Winter K, Burk J. Effects of mesenchymal stromal cells versus serum on tendon healing in a controlled experimental trial in an equine model.. BMC Musculoskelet Disord 2018 Jul 18;19(1):230.
- Fazeli Z, Abedindo A, Omrani MD, Ghaderian SMH. Mesenchymal Stem Cells (MSCs) Therapy for Recovery of Fertility: a Systematic Review.. Stem Cell Rev Rep 2018 Feb;14(1):1-12.
- Fang J, Wei Y, Lv C, Peng S, Zhao S, Hua J. CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling.. Sci Rep 2017 Mar 3;7:43851.
- Jasmin, de Souza GT, Louzada RA, Rosado-de-Castro PH, Mendez-Otero R, Campos de Carvalho AC. Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations.. Int J Nanomedicine 2017;12:779-793.
- Scharf A, Holmes SP, Thoresen M, Mumaw J, Stumpf A, Peroni J. MRI-Based Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem Cells in a Model of Equine Tendonitis.. Stem Cells Int 2016;2016:8610964.
- Berner D, Brehm W, Gerlach K, Gittel C, Offhaus J, Paebst F, Scharner D, Burk J. Longitudinal Cell Tracking and Simultaneous Monitoring of Tissue Regeneration after Cell Treatment of Natural Tendon Disease by Low-Field Magnetic Resonance Imaging.. Stem Cells Int 2016;2016:1207190.
- Geburek F, Mundle K, Conrad S, Hellige M, Walliser U, van Schie HT, van Weeren R, Skutella T, Stadler PM. Tracking of autologous adipose tissue-derived mesenchymal stromal cells with in vivo magnetic resonance imaging and histology after intralesional treatment of artificial equine tendon lesions--a pilot study.. Stem Cell Res Ther 2016 Feb 1;7:21.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
