Evaluation of use of dimethyl sulfoxide for intra-articular lavage in clinically normal horses.
Abstract: The antebrachiocarpal and tarsocrural joints of 10 adult horses were randomly assigned to 1 of 4 groups. Groups were formulated and were treated as follows: group 1, control (arthrocentesis only); group 2, buffered lactated Ringer solution; group 3, 10% dimethyl sulfoxide (DMSO; w/v) in lactated Ringer solution; and group 4, 30% DMSO (w/v) in lactated Ringer solution. Joints were lavaged once with the respective solution. Prior to lavage and on days 1, 4, and 8 after lavage, all horses were evaluated for lameness and joint effusion; synovial fluid total and differential WBC counts, synovial fluid total protein concentration, and mucin clot quality were determined. Horses were euthanatized on day 8, and joints were evaluated grossly, histologically, and histochemically. Significant difference was not observed in effect of lactated Ringer solution, 10% DMSO, and 30% DMSO on any measured variable. At 24 hours after treatment, significant (P less than 0.05) difference in synovial fluid WBC numbers and total protein concentration was detected between control and treated joints. Eighty percent of lavaged joints had effusion 24 hours after treatment, compared with 30% of control joints. Gross, histopathologic, or histochemical differences were not detected between treated and control joints. Results of the study indicate that buffered lactated Ringer, 10% DMSO, and 30% DMSO solutions induce similar inflammatory changes in articular structures and significantly greater inflammatory reaction than does arthrocentesis alone.
Publication Date: 1991-02-01 PubMed ID: 2012346
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study investigated the effects of using different concentrations of Dimethyl Sulfoxide (DMSO) for intra-articular lavage in horses. The authors conclude that buffered lactated Ringer, 10% DMSO, and 30% DMSO solutions create similar inflammatory responses in joints and significantly higher inflammation than arthrocentesis alone.
Study Design
- The researchers selected ten adult horses for the study.
- The horses’ antebrachiocarpal and tarsocrural joints were randomly divided into four groups.
- Group 1 was the control and only underwent arthrocentesis, a procedure to remove joint fluid for diagnosis.
- Group 2 was treated with buffered lactated Ringer Solution; Group 3 with a 10% DMSO solution and Group 4 with a 30% DMSO solution in lactated Ringer solution.
- All these groups were washed once with the corresponding solution.
Procedure and Evaluation
- Prior to the lavage and on days 1, 4, and 8 following the procedure, all the horses were examined for lameness and joint effusion – a condition wherein excess joint fluid is accumulated.
- The synovial fluid’s total and differential White Blood Cells (WBC) counts, total protein concentration, and mucin clot quality – a measure of synovial fluid inflammation – were also determined.
- All the horses were euthanized on day 8, and a gross, histological, and histochemical analysis of the joints was performed.
Findings
- It was observed that there were no significant differences in the effects of lactated Ringer solution, 10% DMSO, and 30% DMSO on any measured variable.
- 24 hours following the treatment, a significant difference in synovial fluid WBC counts and total protein concentration was noted between the control and treated joints.
- Also, 80% of the joints that had undergone lavage displayed effusion 24 hours post-treatment, in contrast with only 30% of the control joints.
- No significant gross, histopathological, or histochemical differences were noticed between the treated and control joints.
Conclusion
- The researchers inferred that using buffered lactated Ringer solution, 10% DMSO, and 30% DMSO for intra-articular lavage in horses results in similar inflammatory changes in the joints.
- They also noticed that these solutions caused a significantly stronger inflammatory reaction compared to just performing arthrocentesis.
Cite This Article
APA
Adair HS, Goble DO, Vanhooser S, Blackford JT, Rohrbach BW.
(1991).
Evaluation of use of dimethyl sulfoxide for intra-articular lavage in clinically normal horses.
Am J Vet Res, 52(2), 333-336.
Publication
Researcher Affiliations
- Department of Rural Practice, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071.
MeSH Terms
- Animals
- Dimethyl Sulfoxide / pharmacology
- Evaluation Studies as Topic
- Injections, Intra-Articular / veterinary
- Isotonic Solutions
- Random Allocation
- Ringer's Solution
- Synovial Fluid / chemistry
- Therapeutic Irrigation
Citations
This article has been cited 2 times.- Sotelo EDP, Vendruscolo CP, Fülber J, Seidel SRT, Jaramillo FM, Agreste FR, Silva LCLCD, Baccarin RYA. Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses-Clinical and Laboratorial Aspects. Vet Sci 2020 Apr 30;7(2).
- Francoz D, Desrochers A, Latouche JS. Effect of repeated arthrocentesis and single joint lavage on cytologic evaluation of synovial fluid in 5 young calves. Can J Vet Res 2007 Apr;71(2):129-34.
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