Evaluation of Villus Synovium From Unaffected Metacarpophalangeal Joints of Adult and Juvenile Horses.
Abstract: Horses are a widely accepted model for osteoarthritis (OA) research. Synovial tissue sampling is commonly used in studies to evaluate and grade the progress of OA or to assess treatment effects. Synovial explants play an important role in ex-vivo studies, increasingly replacing the use of living animals. To understand histomorphological changes in the process of joint-related diseases such as OA, detailed information about histomorphometric parameters of unaffected synovial villi is necessary. The objective of the present study was to evaluate the mean width of the intimal synovial lining and its cellularity as well as the vascularization of the subintimal layer in juvenile and adult horses not affected by a joint-related disease. One hundred synovial samples from both metacarpophalangeal joints from 25 horses (one day to 24 years old) were collected to evaluate the following parameters on digitalized hematoxylin-eosin stained samples: Width of intimal synovial lining measured by the distance from the inner joint surface to the subintimal layer; density of the cells making up the intimal synovial lining by counting cell nuclei; vascularization of the subintimal layer measured by the number and size of vessels in relation to the subintimal area. The median width of the intimal lining did not differ among juvenile (22.34 µm) and adult (23.34 µm) horses. The cellularity of the intimal lining was significantly lower in juvenile (one cell/143.8 µm) than in adult (one cell /188.7µm), (P < .001) horses as well as the density of blood vessels per mm within the subintimal layer (juveniles 1/mm vs. adults 0.05/mm), (P < .001). This study provides morphometric data regarding synovial intimal width, intimal cellularity, and vascularization of equine synovial villi of unaffected horses. For future studies, age-related characteristics should be taken into consideration when synovial tissue samples are used for in-vivo and in-vitro studies.
Copyright © 2021. Published by Elsevier Inc.
Publication Date: 2021-04-29 PubMed ID: 34119205DOI: 10.1016/j.jevs.2021.103637Google Scholar: Lookup
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- Journal Article
Summary
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The research article investigates the structural differences in the synovial tissues of juvenile and adult horses unaffected by joint-related diseases, in order to serve as a basis for future osteoarthritis studies.
Objective and Methodology
- The study is aimed at understanding the thickness of the synovial lining in joints, the cell density of this lining, and the blood supply to the underlying layer of synovial tissue in horses without any joint-related issues. This analysis is meant to provide a baseline for future investigations into joint diseases like osteoarthritis in horses.
- Experiments were conducted by sampling synovial tissues from the metacarpophalangeal joints of 25 horses varying in age from a single day to 24 years. The collected samples were then stained to distinguish different parts of synovial tissue and examined to measure the parameters of interest.
Findings
- The study found that the thickness of the synovial lining remained consistent in both juvenile and adult horses. This indicates that the synovial lining does not thicken as the horse matures.
- However, there were significant differences detected in cellularity and vascularization between young and adult horses. Juvenile horses had lower cell density in the synovial lining and fewer blood vessels per mm in the underlying layer compared to adult horses. This suggests that as horses age, there is an increase in the concentration of cells and blood vessels in the synovial tissue.
Implications for Future Research
- These findings highlight the need to consider age-related changes when using synovial tissue samples in research. Since the composition and characteristics of synovial lining can shift as horses mature, age could potentially influence the outcomes of both in-vivo and in-vitro studies.
- This study provides a foundation for future osteoarthritis research in horses and underscores the importance of understanding the normal physiological conditions before studying pathological ones.
Cite This Article
APA
Della Tommasa S, Winter K, Seeger J, Spitzbarth I, Brehm W, Troillet A.
(2021).
Evaluation of Villus Synovium From Unaffected Metacarpophalangeal Joints of Adult and Juvenile Horses.
J Equine Vet Sci, 102, 103637.
https://doi.org/10.1016/j.jevs.2021.103637 Publication
Researcher Affiliations
- Deparment for horses, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany. Electronic address: della.tommasa@vetmed.uni-leipzig.de.
- Institute of Anatomy, Medical Faculty, Leipzig University, Leipzig, Germany.
- Institute of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany.
- Institute of Veterinary Pathology, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany.
- Deparment for horses, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany.
- Deparment for horses, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany.
MeSH Terms
- Animals
- Duodenum
- Horse Diseases
- Horses
- Joint Diseases / veterinary
- Metacarpophalangeal Joint
- Osteoarthritis / veterinary
- Synovial Membrane
Citations
This article has been cited 4 times.- Farì G, Megna M, Scacco S, Ranieri M, Raele MV, Noya EC, Macchiarola D, Bianchi FP, Carati D, Gnoni A, Inchingolo AD, Qorri E, Scarano A, Scacco A, Arrigoni R, Rapone B. Effects of Terpenes on the Osteoarthritis Cytokine Profile by Modulation of IL-6: Double Face versus Dark Knight?. Biology (Basel) 2023 Jul 28;12(8).
- Farì G, Megna M, Scacco S, Ranieri M, Raele MV, Chiaia Noya E, Macchiarola D, Bianchi FP, Carati D, Panico S, Di Campi E, Gnoni A, Scacco V, Inchingolo AD, Qorri E, Scarano A, Rapone B. Hemp Seed Oil in Association with β-Caryophyllene, Myrcene and Ginger Extract as a Nutraceutical Integration in Knee Osteoarthritis: A Double-Blind Prospective Case-Control Study. Medicina (Kaunas) 2023 Jan 18;59(2).
- Della Tommasa S, Brehm W, Farì G, Bernetti A, Imperante A. Use of Autologous Conditioned Serum (ACS) for Osteoarthritis Treatment in Horses: A Systematic Review of Clinical Data. Vet Sci 2023 Dec 18;10(12).
- Bernetti A, Agostini F, Paoloni M, Raele MV, Farì G, Megna M, Mangone M. Could Hyaluronic Acid Be Considered as a Senomorphic Agent in Knee Osteoarthritis? A Systematic Review. Biomedicines 2023 Oct 22;11(10).
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