Evidence supporting an increased presence of reactive oxygen species in the diseased equine joint.
Abstract: Reactive oxygen species (ROS) are capable of degrading many components of the joint in the presence of insufficient antioxidant defences, and as a result have been implicated in the pathogenesis of joint disease in horses. However, to our knowledge, evidence of ROS occurring in diseased joints of horses has not been reported. The objective of this experiment was to compare differences in synovial fluid protein carbonyl content (as a marker of oxidative modification of synovial fluid proteins by ROS) and the antioxidant status of synovial fluid between clinically normal and diseased equine joints. Synovial fluid was collected from the metacarpophalangeal, metatarsophalangeal, carpal and tarsal joints of 4 horses, age 2-5 years, as controls, and from diseased joints (metacarpophalangeal, metatarsophalangeal, carpal, tarsal and/or femoropatellar) of 61 horses, age 2-5 years. Synovial fluid protein carbonyl content was higher (P<0.01) in diseased joints as compared to controls. Antioxidant status of synovial fluid from diseased joints was higher, but not significantly, than that of controls (P = 0.0595). These findings require further study to determine their contribution to the overall disease process.
Publication Date: 2000-10-19 PubMed ID: 11037267DOI: 10.2746/042516400777591129Google Scholar: Lookup
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- Journal Article
- Antioxidants
- Carpal Joint
- Clinical Study
- Comparative Study
- Diagnosis
- Disease Diagnosis
- Disease Etiology
- Equine Health
- Horses
- Inflammation
- Joint Health
- Metacarpophalangeal Joint
- Metatarsophalangeal Joint
- Oxidative Stress
- Pathogenesis
- Reactive Oxygen Species
- Synovial Fluid
- Tarsal Joint
- Veterinary Medicine
- Veterinary Research
Summary
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The study explores the correlation between increased reactive oxygen species (ROS) and joint disease in horses. Using various methods, the researchers found evidence of higher synovial fluid protein carbonyl content (a ROS marker) and antioxidant presence in diseased equine joints compared to healthy ones.
Objective of the Research
The study’s goal was to explore the possible role of ROS in the development of joint diseases in horses. So far, there were no reported instances of ROS appearing in diseased joints of horses. Therefore, this study focused on comparing the protein carbonyl content (a marker of oxidative modification) and the antioxidant status in synovial fluid between normal and diseased equine joints.
Methods Used in the Study
- Synovial fluid, the lubricant of the joints, was collected from various joints of control and diseased horses, all aged between 2-5 years.
- Four horses with healthy joints served as controls, while synovial fluid was collected from the diseased joints of 61 horses as the research subjects.
Results of the Experiment
- The researchers found that synovial fluid protein carbonyl content, an indicator of ROS activity, was significantly higher in diseased equine joints versus the control group.
- Notably, the antioxidant status in the synovial fluid of diseased joints was also higher, indicating the body’s response to oxidative stress, though it wasn’t significantly different from the control group.
Conclusions and Future Directions
- The results of this study suggest an increased presence of ROS in diseased equine joints, which supports the hypothesis that ROS might play a role in the pathogenesis of joint disease in horses.
- Although these findings contribute to our understanding of equine joint diseases, further investigations are needed to determine the exact contributions of ROS and antioxidant responses to disease processes.
Cite This Article
APA
Dimock AN, Siciliano PD, McIlwraith CW.
(2000).
Evidence supporting an increased presence of reactive oxygen species in the diseased equine joint.
Equine Vet J, 32(5), 439-443.
https://doi.org/10.2746/042516400777591129 Publication
Researcher Affiliations
- Department of Animal Sciences Colorado State University, Fort Collins 80523, USA.
MeSH Terms
- Animals
- Antioxidants / analysis
- Horse Diseases / metabolism
- Horses
- Joint Diseases / metabolism
- Joint Diseases / veterinary
- Proteins / analysis
- Reactive Oxygen Species
- Synovial Fluid / chemistry
Citations
This article has been cited 12 times.- Nocera I, Sgorbini M, Meucci V, Gracia-Calvo LA, Tapio H, Camisi M, Sala G, Citi S. Procalcitonin and carbonylated protein concentrations in equine synovial fluid. Vet Res Commun 2024 Apr;48(2):1263-1269.
- Nocera I, Bonelli F, Meucci V, Rinnovati R, Spadari A, Intorre L, Pretti C, Sgorbini M. Evaluation of Protein Carbonyl Content in Healthy and Sick Hospitalized Horses. Front Vet Sci 2020;7:582886.
- Donnell JR, Frisbie DD. Use of firocoxib for the treatment of equine osteoarthritis. Vet Med (Auckl) 2014;5:159-168.
- Żak A, Siwińska N, Chełmecka E, Bażanów B, Romuk E, Adams A, Niedźwiedź A, Stygar D. Effects of Advanced Age, Pituitary Pars Intermedia Dysfunction and Insulin Dysregulation on Serum Antioxidant Markers in Horses. Antioxidants (Basel) 2020 May 21;9(5).
- Tsuzuki N, Kanbayashi Y, Kusano K. Markers for oxidative stress in the synovial fluid of Thoroughbred horses with carpal bone fracture. J Equine Sci 2019 Mar;30(1):13-16.
- Bourebaba L, Röcken M, Marycz K. Osteochondritis dissecans (OCD) in Horses - Molecular Background of its Pathogenesis and Perspectives for Progenitor Stem Cell Therapy. Stem Cell Rev Rep 2019 Jun;15(3):374-390.
- Celi P, Gabai G. Oxidant/Antioxidant Balance in Animal Nutrition and Health: The Role of Protein Oxidation. Front Vet Sci 2015;2:48.
- McIlwraith CW, Frisbie DD, Kawcak CE. The horse as a model of naturally occurring osteoarthritis. Bone Joint Res 2012 Nov;1(11):297-309.
- Lamprecht ED, Williams CA. Biomarkers of antioxidant status, inflammation, and cartilage metabolism are affected by acute intense exercise but not superoxide dismutase supplementation in horses. Oxid Med Cell Longev 2012;2012:920932.
- Benbarek H, Ayad A, Deby-Dupont G, Boukraa L, Serteyn D. Modulatory effects of non-steroidal anti-inflammatory drugs on the luminol and lucigenin amplified chemiluminescence of equine neutrophils. Vet Res Commun 2012 Mar;36(1):29-33.
- Villasante A, Araneda OF, Behn C, Galleguillos M, Adarmes H. Antioxidant capacity and oxidative damage determination in synovial fluid of chronically damaged equine metacarpophalangeal joint. Vet Res Commun 2010 Feb;34(2):133-41.
- Lejeune JP, Serteyn D, Gangl M, Schneider N, Deby-Dupont G, Deberg M, Henrotin Y. Plasma concentrations of a type II collagen-derived peptide and its nitrated form in growing Ardenner sound horses and in horses suffering from juvenile digital degenerative osteoarthropathy. Vet Res Commun 2007 Jul;31(5):591-601.
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