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Veterinary surgery : VS2024; doi: 10.1111/vsu.14150

Ex vivo comparison of one-layer versus two-layer closures in end-to-end anastomoses in normal equine descending colon.

Abstract: The aim of the study was to evaluate time to closure, bursting pressures and luminal diameters of a single and double-layer end-to-end anastomoses in normal equine descending colon. Methods: Experimental study. Methods: Eight adult horses. Methods: Four segments of descending colon from each horse were randomly assigned to a control (n = 8, CON), one-layer (n = 12, group 1; continuous Lembert pattern), or two-layer group (n = 12, group 2; simple continuous oversewn with Cushing pattern). Anastomoses were performed, and time to closure and luminal diameter were measured. Bursting pressures were determined, and location of failure was recorded. Mixed analysis of variance (ANOVA) was employed. Results: Mean time to completion was decreased (p = .003) in group 1 (18.6 min ± 22.8 s) compared with group 2 (21.35 min ± 22.8 s). Luminal diameter was reduced (p < .0001) in group 2 (47 ± 2.46 mm) compared to group 1 (65 ± 2.77 mm). Location of failure was remote to the anastomosis in all but one segment; therefore, differences in anastomotic bursting pressures could not be determined. Conclusions: Two-layer closures resulted in significant luminal reduction and took longer to complete than one-layer anastomoses. Conclusions: Use of one-layer closures may be advantageous primarily with respect to luminal diameter.
© 2024 The American College of Veterinary Surgeons.
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Publication Date: 2024-09-05 PubMed ID: 39235049DOI: 10.1111/vsu.14150Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research compares the differences between one-layer and two-layer surgical closures in the descending colon of horses. The study found that one-layer closures took less time to perform and resulted in a wider luminal diameter, suggesting potential advantages over two-layer closures.

Objective and Methods

  • The research aimed to assess the efficiency and outcomes of one-layer versus two-layer surgical closures in the descending colon of horses. Specifically, it focused on the time it takes to perform the closure, the resilience of the closure as measured by its bursting pressure, and the resultant diameter of the colon’s lumen.
  • The study used eight adult horses and separated four segments of each horse’s descending colon into three categories: a control group (eight segments), a one-layer group (twelve segments) with a continuous Lembert pattern, and a two-layer group (twelve segments) with a simple continuous pattern oversewn with a Cushing pattern.
  • After performing the closures, the time to closure, lumen diameter, and bursting pressures were measured and recorded. The points of failure were also noted down. Mixed analysis of variance (ANOVA) was used for the analysis of the results.

Findings

  • The study revealed that one-layer closures took less time, with an average duration of 18.6 minutes, compared to the two-layer closure method, which took around 21.35 minutes. This implies that one-layer closures are more time-efficient in surgical settings.
  • Moreover, the one-layer closures resulted in a wider luminal diameter (an average of 65mm) compared to the two-layer closures (an average of 47mm). A wider lumen might promote better bowel function post-surgery as it facilitates smoother passing of contents.
  • The bursting pressures of the closures were not compared due to consistent remote failures, showing that failures did not typically occur at the site of the anastomosis.

Conclusions

  • The two-layer closure method took more time and resulted in smaller luminal diameters than the one-layer method in the equine descending colon.
  • The study concluded that one-layer closures might be beneficial over two-layer closures primarily due to the larger luminal diameter post-surgery. However, the resilience of closures, as determined by bursting pressures, could not be compared in this study.

Cite This Article

APA
St Blanc MP, Mirza MH, Riggs LM, Leise BS. (2024). Ex vivo comparison of one-layer versus two-layer closures in end-to-end anastomoses in normal equine descending colon. Vet Surg. https://doi.org/10.1111/vsu.14150

Publication

Veterinary surgery : VS
ISSN: 1532-950X
NlmUniqueID: 8113214
Country: United States
Language: English

Researcher Affiliations

St Blanc, Michael P
  • Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA.
Mirza, Mustajab H
  • Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA.
Riggs, Laura M
  • Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA.
Leise, Britta S
  • Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, Louisiana, USA.

Grant Funding

  • School of Veterinary Medicine, Louisiana State University LSU Department of Veterinary Clinical Sciences Com

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