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Home / Equine Research Bank / BMC Vet Res / Ex vivo comparison of sliding knot ligatures vs. haemostatic...
BMC veterinary research2020; 16(1); 290; doi: 10.1186/s12917-020-02498-x

Ex vivo comparison of sliding knot ligatures vs. haemostatic clips for equine small intestinal mesenteric vessel occlusion.

Abstract: In equine abdominal surgery, resection and anastomosis of strangulated intestine is a commonly performed procedure. To date, ligatures, vessel sealing devices and the ligate-divide stapler have been described for this use in horses. The objective of this study was to compare the application of haemostatic clips and ligatures to occlude equine mesenteric vessels. Portions of jejunum with ten associated mesenteric vessels were collected from 12 horses at a local abattoir and divided into two groups. Portions of intestine were divided into two sections comprising five vessels each and assigned to Group A or Group B. Each vessel was occluded with a triple ligature. In Group A, vessels were ligated with three circumferential ligatures tied with a sliding knot with two overthrows. In Group B, vessels were occluded with application of three haemoclips. The procedures were performed by the same experienced surgeon. Intestinal length, construction time and vessel leaking pressure were measured and compared between groups. Results: The intestinal length (mean ± SD) was 3.78 ± 0.43 m in Group A and 3.04 ± 0.83 m in Group B. The difference was not significant (p = 0.297). The construction time (mean ± SD) was 7.03 ± 0.34 min in Group A and 2.40 ± 0.43 min in Group B. The difference was significant (p < 0.0001). The leaking pressure was 1000 (750-1050) mmHg (median, IQ range) in Group A and 1050 (800-1050) mmHg (median, IQ range) in Group B. The difference was not significant (p = 0.225). Conclusions: Haemoclip application is comparable in terms of leaking pressure but quicker than sliding knots to apply.
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Publication Date: 2020-08-12 PubMed ID: 32787840PubMed Central: PMC7424996DOI: 10.1186/s12917-020-02498-xGoogle Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the differences between the use of sliding knot ligatures and haemostatic clips in equine abdominal surgery, specifically in occluding small intestinal mesenteric vessels. The study shows haemostatic clips as an efficient method comparable to sliding knot ligatures, with the benefit of less application time.

Research Design and Method

  • The research was conducted in an in vitro setting using portions of jejunum sourced from 12 horses at a local abattoir.
  • The specimens were then divided into two groups with equal mesenteric vessels to ensure fairness in comparison.
  • The same experienced surgeon performed the occlusion procedures in both methods, nullifying differential expertise as a variable.

Procedure for the Two Groups

  • In Group A, the mesenteric vessels were occluded using three circumferential ligatures tied with a sliding knot.
  • In Group B, haemostatic clips were used to occlude the mesenteric vessels in an identical fashion.

Key Measures

  • With each method, various factors were evaluated, including the length of the intestine, the construction time, and the pressure at which leaking occurred.

Results and Conclusion

  • The study found no significant difference in the length of the intestine or leaking pressure between the two methods.
  • However, construction time was significantly lower when using haemostatic clips compared to sliding knot ligatures.
  • The findings strongly suggest that the haemostatic clip method could be a valuable alternative to the traditional sliding knot ligature in equine abdominal surgery due to its efficacy and time-saving properties.

Cite This Article

APA
Giusto G, Gandini M. (2020). Ex vivo comparison of sliding knot ligatures vs. haemostatic clips for equine small intestinal mesenteric vessel occlusion. BMC Vet Res, 16(1), 290. https://doi.org/10.1186/s12917-020-02498-x

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 16
Issue: 1
Pages: 290
PII: 290

Researcher Affiliations

Giusto, Gessica
  • Department of Veterinary Sciences, University of Turin, Largo Braccini 2-5, 10095, Grugliasco, TO, Italy. gessica.giusto@unito.it.
Gandini, Marco
  • Department of Veterinary Sciences, University of Turin, Largo Braccini 2-5, 10095, Grugliasco, TO, Italy.

MeSH Terms

  • Animals
  • Hemostasis, Surgical / instrumentation
  • Hemostasis, Surgical / methods
  • Hemostasis, Surgical / veterinary
  • Horses / surgery
  • Jejunum / surgery
  • Ligation / methods
  • Ligation / veterinary
  • Mesenteric Arteries / surgery
  • Mesenteric Veins / surgery
  • Pressure

Grant Funding

  • R01 CA092108 / NCI NIH HHS

Conflict of Interest Statement

The authors declare that they have no competing interests.

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This article includes 17 references
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