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Human gene therapy2009; 20(12); 1697-1702; doi: 10.1089/hum.2009.030

Ex vivo serotype-specific transduction of equine joint tissue by self-complementary adeno-associated viral vectors.

Abstract: Cell transplantation for the treatment of joint disease is an important clinical tool. Genetic modification of cells before transplantation has shown enhanced healing. Ex vivo genetic modification of joint tissue cells with various adeno-associated virus (AAV) serotypes has not been investigated. The transduction efficiencies of self-complementary AAV serotypes (1-6 and 8) were determined in joint tissue containing chondrocytes and synoviocytes isolated from equine models. When comparing scAAV serotypes for efficient transduction ex vivo, in chondrocytes versus synoviocytes, serotypes 6 and 2, and serotypes 3 and 2, respectively, appeared superior for gene expression. Unlike adenoviral vectors, no upregulation of inflammatory markers, such as matrix metalloproteinases and aggrecanase, was seen on treatment of joint tissue with AAV vectors ex vivo. Our findings also corroborate that ex vivo transduction of joint tissue can result in high transgene protein levels over time, and transplantation modalities might be feasible using AAV vectors in the treatment of joint-related diseases.
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Publication Date: 2009-08-01 PubMed ID: 19642864PubMed Central: PMC2861962DOI: 10.1089/hum.2009.030Google Scholar: Lookup
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  • Journal Article
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper describes a study involving the use of certain types of viruses to modify cells ex vivo (outside the body), potentially improving treatment of joint diseases. Particularly, the study examined the effectiveness of various serotypes of adeno-associated virus (AAV) in modifying cells from horse joint tissue.

Objective of the Study

  • The primary objective of this study was to analyze the ability of various serotypes (or variations) of adeno-associated virus (AAV) to modify cells found in equine (horse) joint tissue outside of the body. This process is known as ex vivo serotype-specific transduction.

Methodology

  • The researchers tested a set of self-complementary AAV serotypes (1-6 and 8) on joint tissue containing chondrocytes and synoviocytes. These are types of cells found in joints that play a role in joint health and disease.
  • The process of genetic modification was conducted outside the living body, thus making it an ‘ex vivo’ process.
  • The effectiveness of the serotypes was determined by their efficiency in cell transduction, or the ability to introduce new genes into cells.

Results and Findings

  • The study pointed out that certain AAV serotypes were superior in terms of their transduction efficiency. In the case of chondrocytes, serotypes 6 and 2 showed high efficiency, while for synoviocytes, serotypes 3 and 2 were superior.
  • Unlike treatments involving adenoviral vectors, using AAV vectors did not lead to an increase in inflammatory markers, like matrix metalloproteinases and aggrecanase.
  • The research also showed that ex vivo transduction of joint tissue can result in high transgene protein levels over time. This finding suggests that cell transplantation might be improved through cell modification via AAV vectors, making it a potentially effective treatment for joint-related diseases.

Cite This Article

APA
Goodrich LR, Choi VW, Carbone BA, McIlwraith CW, Samulski RJ. (2009). Ex vivo serotype-specific transduction of equine joint tissue by self-complementary adeno-associated viral vectors. Hum Gene Ther, 20(12), 1697-1702. https://doi.org/10.1089/hum.2009.030

Publication

Human gene therapy
ISSN: 1557-7422
NlmUniqueID: 9008950
Country: United States
Language: English
Volume: 20
Issue: 12
Pages: 1697-1702

Researcher Affiliations

Goodrich, L R
  • Orthopaedic Research Center, College of Veterinary Medicine, Colorado State University, Fort Collins, CO 80523, USA.
Choi, V W
    Carbone, B A Duda
      McIlwraith, C W
        Samulski, R J

          MeSH Terms

          • Animals
          • Chondrocytes / cytology
          • Chondrocytes / transplantation
          • Dependovirus / classification
          • Dependovirus / genetics
          • Genetic Vectors / classification
          • Genetic Vectors / genetics
          • Horses
          • Serotyping
          • Synovial Membrane / cytology
          • Synovial Membrane / transplantation
          • Transduction, Genetic / methods

          Grant Funding

          • 1K08AR054903-01A2 / NIAMS NIH HHS

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          This article has been cited 25 times.
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