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Metabolites2020; 10(7); 298; doi: 10.3390/metabo10070298

Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by 1H-NMR.

Abstract: In the present study, data related to the metabolomics of saliva and serum in trained standardbred horses are provided for the first time. Metabolomic analysis allows to analyze all the metabolites within selected biofluids, providing a better understanding of biochemistry modifications related to exercise. On the basis of the current advances observed in metabolomic research on human athletes, we aimed to investigate the metabolites' profile of serum and saliva samples collected from healthy standardbred horses and the relationship with physical exercise. Twelve trained standardbred horses were sampled for blood and saliva before (T) and immediately after (T) standardized exercise. Metabolomic analysis of both samples was performed by H-NMR spectroscopy. Forty-six metabolites in serum and 62 metabolites in saliva were detected, including alcohols, amino acids, organic acids, carbohydrates and purine derivatives. Twenty-six and 14 metabolites resulted to be significantly changed between T and T in serum and saliva, respectively. The findings of 2-hydroxyisobutyrate and 3-hydroxybutyrate in serum and GABA in equine saliva, as well as their modifications following exercise, provide new insights about the physiology of exercise in athletic horses. Glycerol might represent a novel biomarker for fitness evaluation in sport horses.
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Publication Date: 2020-07-21 PubMed ID: 32708237PubMed Central: PMC7407172DOI: 10.3390/metabo10070298Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper discusses a study conducted on the changes in saliva and serum metabolites in trained standardbred horses after they exercise, and these changes are analyzed using 1H-NMR spectroscopy. The study found different types of metabolites in both saliva and serum, including alcohols, amino acids, organic acids, carbohydrates, and purine derivatives. The findings suggested that 2-hydroxyisobutyrate, 3-hydroxybutyrate, and GABA could provide insights about the physiology of exercise in horses, and glycerol might be a new biomarker for fitness evaluation in sport horses.

Research Methodology

  • The research was conducted on twelve trained standardbred horses, selected for the study.
  • Saliva and serum samples from these horses were collected at two different time points – before and immediately after they underwent standardized physical exercises.
  • The metabolites in these samples were then analyzed using 1H-NMR spectroscopy, a method used to measure the quantity of metabolites in biofluids.
  • A total of 46 metabolites in serum and 62 metabolites in saliva were detected, encompassing a range of compound types including alcohols, amino acids, organic acids, carbohydrates, and purine derivatives.

Results of the Study

  • There were significant differences between the metabolite composition of serum and saliva samples taken before and after exercise.
  • Taken together, 26 metabolites in the serum and 14 in the saliva showed significant changes after exercise.
  • In the serum samples, 2-hydroxyisobutyrate and 3-hydroxybutyrate were detected, and their levels changed post-exercise. Similarly, the levels of GABA, a neurotransmitter that regulates muscle tone, changed in the saliva samples after exercise.
  • The researchers suggested that the changes in these metabolites might provide new insights into the physiology of exercise in horses.

Significance and Potential Applications of the Study

  • The study provides first-time data on the metabolomics of saliva and serum in trained horses, contributing towards a better understanding of biochemical changes due to exercise.
  • The finding that the levels of 2-hydroxyisobutyrate, 3-hydroxybutyrate, and GABA in serum and saliva respectively change with exercise helps to enhance our understanding of the physiological response to exercise in horses, which could potentially lead to more effective training methods and care for athletic horses.
  • Moreover, the study highlights glycerol – a compound that changed significantly with exercise – as a potential novel biomarker for assessing fitness in sport horses, suggesting possible practical applications of metabolite analysis in equine sport medicine and performance analysis.

Cite This Article

APA
Bazzano M, Laghi L, Zhu C, Lotito E, Sgariglia S, Tesei B, Laus F. (2020). Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by 1H-NMR. Metabolites, 10(7), 298. https://doi.org/10.3390/metabo10070298

Publication

Metabolites
ISSN: 2218-1989
NlmUniqueID: 101578790
Country: Switzerland
Language: English
Volume: 10
Issue: 7
PII: 298

Researcher Affiliations

Bazzano, Marilena
  • School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione, 93/95, 62024 Matelica, Italy.
Laghi, Luca
  • Department of Agricultural and Food Sciences, University of Bologna, 47521 Cesena, Italy.
Zhu, Chenglin
  • Department of Agricultural and Food Sciences, University of Bologna, 47521 Cesena, Italy.
Lotito, Enrica
  • School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione, 93/95, 62024 Matelica, Italy.
Sgariglia, Stefano
  • Practitioner, 63900 Fermo, Italy.
Tesei, Beniamino
  • School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione, 93/95, 62024 Matelica, Italy.
Laus, Fulvio
  • School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione, 93/95, 62024 Matelica, Italy.

Conflict of Interest Statement

The authors declare no conflict of interest.

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