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Osteoarthritis and cartilage open2023; 5(2); 100354; doi: 10.1016/j.ocarto.2023.100354

Salivary biglycan-neo-epitope-BGN262: A novel surrogate biomarker for equine osteoarthritic sub-chondral bone sclerosis and to monitor the effect of short-term training and surface arena.

Abstract: We aimed to delineate a novel soluble Biglycan Neo-epitope-BGN262 in saliva from young reference and osteoarthritic horses in conjunction with the influence of short-term training exercise, riding surface hardness, circadian rhythm, and feeding on its soluble levels. Unassigned: A custom-made inhibition ELISA was used for the quantification of BGN262 in saliva. Cohort 1: A cross-sectional study comprising reference (N ​= ​19) and OA horses (N ​= ​9) with radiographically classified subchondral bone sclerosis. Receiver operating characteristic curve analysis was performed to evaluate the robustness of BGN262. Cohorts 2 (N ​= ​5) & 3 (N ​= ​7): Longitudinal studies of sampling during a short-term training exercise (sand-fibre) and a cross-over design of short-training exercise on 2 different riding arenas (sand and sand-fibre), respectively. Capillary western immunoassay was used to determine the BGN262 molecular size in a selection of saliva samples collected from cohort 1. Unassigned: Cohort 1: Salivary BGN262 levels were significantly higher in the OA group. The Receiver operating characteristic curve analysis showed an area under the curve of 0.8304 [0.6386 to 1.022], indicating a good separation from the reference group. Cohorts 2 & 3: Salivary BGN262 levels significantly changed during the exercise on sand and sand-fibre arena, with a trend towards higher levels for sand-fibre. The size of the BGN262 fragment determined by Capillary western assay was 18 ​kDa. Unassigned: The data presented show saliva BGN262 levels as a novel biomarker in evaluating the influence of exercise, and interaction with riding arenas alongside assessing osteoarthritis severity.
© 2023 The Author(s).
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Publication Date: 2023-03-15 PubMed ID: 36968250PubMed Central: PMC10033749DOI: 10.1016/j.ocarto.2023.100354Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aimed to identify a new biomarker found in saliva, called Biglycan Neo-epitope-BGN262, that may help evaluate exercise impact, arena riding interaction, and the severity of osteoarthritis, particularly subchondral bone sclerosis, in horses.

Background

  • The study focused on Biglycan Neo-epitope-BGN262 (BGN262), a protein fragment found in saliva.
  • Research aimed to understand BGN262’s behavior relative to short-term exercise, the hardness of riding surfaces, circadian rhythms, and feeding.
  • The researchers also investigated whether BGN262 levels could indicate the presence and the severity of osteoarthritis, particularly subchondral bone sclerosis, in horses.

Methodology

  • An enzyme-linked immunosorbent assay (ELISA), a commonly used lab test to measure the concentration of substances such as proteins and hormones in body fluids, was used to quantify BGN262 in equine saliva.
  • The study included a cross-sectional study (Cohort 1) which consisted of a reference group (19 horses) and an osteoarthritic (OA) group (9 horses) to compare BGN262 levels.
  • Additionally, two longitudinal studies (Cohorts 2 and 3) were conducted, involving a training exercise and a cross-over test between two different types of riding arenas, respectively.
  • The size of the BGN262 fragment was determined using a Capillary Western Assay, a method used to measure proteins in samples.

Findings

  • Salivary BGN262 levels were significantly higher in horses with OA.
  • The use of the Receiver Operating Characteristic (ROC) curve, a measure of the robustness of BGN262, showed good separation from the reference group.
  • Salivary levels of BGN262 also changed significantly during exercises conducted on sand and sand-fibre arenas, and they tended to be higher on sand-fibre.

Implications

  • Results suggested that BGN262 levels can serve as a new biomarker to evaluate the impact of exercise and interaction with various riding arenas on horses.
  • Moreover, the study demonstrated that higher levels of BGN262 in saliva might indicate the severity of osteoarthritis in horses.

Cite This Article

APA
Adepu S, Lord M, Hugoh Z, Nyström S, Mattsson-Hulten L, Abrahamsson-Aurell K, Lützelschwab C, Skiöldebrand E. (2023). Salivary biglycan-neo-epitope-BGN262: A novel surrogate biomarker for equine osteoarthritic sub-chondral bone sclerosis and to monitor the effect of short-term training and surface arena. Osteoarthr Cartil Open, 5(2), 100354. https://doi.org/10.1016/j.ocarto.2023.100354

Publication

Osteoarthritis and cartilage open
ISSN: 2665-9131
NlmUniqueID: 101767068
Country: England
Language: English
Volume: 5
Issue: 2
Pages: 100354
PII: 100354

Researcher Affiliations

Adepu, S
  • Department of Pathology, Institute of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Lord, M
  • Department of Physics, Chalmers University of Technology, Gothenburg, Sweden.
Hugoh, Z
  • Department of Physics, Chalmers University of Technology, Gothenburg, Sweden.
Nyström, S
  • Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska University Hospital, Gothenburg University, Gothenburg, Sweden.
Mattsson-Hulten, L
  • Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska University Hospital, Gothenburg University, Gothenburg, Sweden.
Abrahamsson-Aurell, K
  • Hallands Djursjukhus Kungsbacka Hästklinik, Älvsåkers Byväg 20, 434 95 Kungsbacka, Sweden.
Lützelschwab, C
  • Department of Pathology, Institute of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Skiöldebrand, E
  • Department of Pathology, Institute of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.

Conflict of Interest Statement

ES and LMH are among the stakeholders of SGPTH Life Science holding the patent covering the BGN262 neo-epitope. The other co-authors have no conflicts of interest to declare.

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