Exercise induced upregulation of glutamate-cysteine ligase catalytic subunit and glutamate-cysteine ligase modifier subunit gene expression in Thoroughbred horses.
Abstract: This study was performed to reveal the molecular structure and expression patterns of horse glutamate-cysteine ligase catalytic subunit () and glutamate-cysteine ligase modifier subunit () genes whose products form glutamate cysteine ligase, which were identified as differentially expressed genes in the previous study. Methods: We performed bioinformatics analyses, and gene expression assay with quantitative polymerase chain reaction (qPCR) for horse and genes in muscle and blood leukocytes of Thoroughbred horses. Results: Expression of showed the same pattern in both blood and muscle tissues after exercise. Expression of increased in the muscle and blood of Thoroughbreds, suggesting a tissue-specific regulatory mechanism for the expression of . In addition, expression of the gene increased after exercise in both the blood and muscle of Thoroughbreds. Conclusions: We established the expression patterns of and in the skeletal muscle and blood of Thoroughbred horses in response to exercise. Further study is now warranted to uncover the functional importance of these genes in exercise and recovery in racehorses.
Publication Date: 2017-01-02 PubMed ID: 28111441PubMed Central: PMC5411833DOI: 10.5713/ajas.16.0776Google Scholar: Lookup
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- Journal Article
Summary
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The research investigates the expression patterns of two genes involved in forming glutamate cysteine ligase in horses. The genes’ expression is studied following exercise and seems to differ between muscle and blood tissues.
Objective of the Research
- The primary purpose of this study is to uncover the molecular structure and expression patterns of two distinct genes, glutamate-cysteine ligase catalytic subunit () and glutamate-cysteine ligase modifier subunit (), in Thoroughbred horses. These genes are components of the enzyme glutamate cysteine ligase.
Methods Used
- To comprehend these genes’ structures and expression patterns, the researchers have used certain processes, such as bioinformatics analyses and a gene expression assay with a quantitative polymerase chain reaction (qPCR).
- The focus of these tests was specifically on blood leukocytes and muscle tissues of Thoroughbred horses.
Findings
- The research revealed that the expression of had consistent patterns in both blood and muscle tissues post-exercise.
- The expression was found to increase in the muscle and blood of Thoroughbreds, implying a tissue-specific regulatory approach for the expression.
- On top of these, the expression of the gene also saw an increase after the exercise in both blood and muscle tissues of Thoroughbred horses.
Conclusion and Future Research
- Based on these findings, the research successfully established the expression patterns of and in the skeletal muscle and blood of Thoroughbred horses post-exercise.
- The observations driven from this study can pave the way for future research to understand these genes’ functional significance in exercise and recovery processes in racehorses.
Cite This Article
APA
Park JW, Choi JY, Hong SA, Kim NY, Do KT, Song KD, Cho BW.
(2017).
Exercise induced upregulation of glutamate-cysteine ligase catalytic subunit and glutamate-cysteine ligase modifier subunit gene expression in Thoroughbred horses.
Asian-Australas J Anim Sci, 30(5), 728-735.
https://doi.org/10.5713/ajas.16.0776 Publication
Researcher Affiliations
- Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea.
- Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea.
- Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea.
- National Institute of Animal Science, Rural Development Administration, Jeju 63242, Korea.
- Department of Animal Biotehnology, Faculty of Biotechnology, Jeju National University, Jeju 63243, Korea.
- Department of Animal Biotechnology, Chonbuk National, University, Jeonju 54896, Korea.
- Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea.
Conflict of Interest Statement
. We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.
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Citations
This article has been cited 6 times.- Park JW, Kim KH, Choi JK, Park TS, Song KD, Cho BW. Regulation of toll-like receptors expression in muscle cells by exercise-induced stress. Anim Biosci 2021 Oct;34(10):1590-1599.
- Lee HG, Choi JY, Park JW, Park TS, Song KD, Shin D, Cho BW. Effects of exercise on myokine gene expression in horse skeletal muscles. Asian-Australas J Anim Sci 2019 Mar;32(3):350-356.
- Zeng N, D'Souza RF, Sorrenson B, Merry TL, Barnett MPG, Mitchell CJ, Cameron-Smith D. The putative leucine sensor Sestrin2 is hyperphosphorylated by acute resistance exercise but not protein ingestion in human skeletal muscle. Eur J Appl Physiol 2018 Jun;118(6):1241-1253.
- Choi JY, Jang HJ, Park JW, Oh JD, Shin D, Kim NY, Oh JH, Song KD, Cho BW. Characterization of gene expression and genetic variation of horse ERBB receptor feedback inhibitor 1 in Thoroughbreds. Asian-Australas J Anim Sci 2018 Mar;31(3):309-315.
- Cheng X, Park N, Lee Y. Metal-Based Nanomedicines for Inducing Programmed Cell Death to Enhance the Efficacy of Cancer Immunotherapy. Int J Nanomedicine 2025;20:16055-16092.
- Zhu J, Zhang Z, Chu Y, Xie Z, Zeng D, Jin L, Li L. Application of Nanomaterial-Mediated Ferroptosis Regulation in Kidney Disease. Int J Nanomedicine 2025;20:1637-1659.
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