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Home / Equine Research Bank / Vet J / Expansion under hypoxic conditions enhances the chondrogenic...
Veterinary journal (London, England : 1997)2012; 195(2); 248-251; doi: 10.1016/j.tvjl.2012.06.008

Expansion under hypoxic conditions enhances the chondrogenic potential of equine bone marrow-derived mesenchymal stem cells.

Abstract: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are widely used in regenerative medicine in horses. Most of the molecular characterisations of BM-MSCs have been made at 20% O(2), a higher oxygen level than the one surrounding the cells inside the bone marrow. The present work compares the lifespan and the tri-lineage potential of equine BM-MSCs expanded in normoxia (20% O(2)) and hypoxia (5% O(2)). No significant differences were found in long-term cultures for osteogenesis and adipogenesis between normoxic and hypoxic expanded BM-MSCs. An up-regulation of the chondrogenesis-related genes (COL2A1, ACAN, LUM, BGL, and COMP) and an increase of the extracellular sulphated glycosaminoglycan content were found in cells that were expanded under hypoxia. These results suggest that the expansion of BM-MSCs in hypoxic conditions enhances chondrogenesis in equine BM-MSCs.
Copyright © 2012 Elsevier Ltd. All rights reserved.
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Publication Date: 2012-07-06 PubMed ID: 22771146DOI: 10.1016/j.tvjl.2012.06.008Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how growing bone marrow-derived stem cells in low-oxygen conditions can improve their potential to turn into cartilage cells in horses.

Objective of the Research

The aim of the research was to discern if the conditions under which equine bone marrow-derived mesenchymal stem cells (BM-MSCs) were grown affected their potential to differentiate into different cell types. The researchers specifically compared the effects of normoxia (normal oxygen levels, 20% O2) and hypoxia (low oxygen levels, 5% O2) on the cells.

Methodology

  • The researchers cultivated equine BM-MSCs under two different oxygen conditions – normoxia and hypoxia.
  • The research team then looked at the lifespan and the potential of these cells to develop into three types of cells – bone cells (osteogenesis), fat cells (adipogenesis), and cartilage cells (chondrogenesis).

Findings

  • The study discovered no discernible differences in the ability of the cells to develop into bone or fat cells when cultivated under either oxygen conditions over a long period.
  • However, there was an observable difference when it came to the potential of the cells to become cartilage cells.
  • Equine BM-MSCs that were grown under hypoxic conditions presented an up-regulation, meaning an increase in the activity of the genes associated with chondrogenesis, namely COL2A1, ACAN, LUM, BGL, and COMP.
  • There was also a rise in the content of extracellular sulphated glycosaminoglycan (a molecule related to cartilage tissue production) in these hypoxia-conditioned cells.

Conclusions

The study hence implies that growing BM-MSCs under low-oxygen conditions might enhance their ability to differentiate into cartilage cells. This finding could bear significance for regenerative medicine in horses, particularly for conditions needing enhanced cartilage repair.

Cite This Article

APA
Ranera B, Remacha AR, Álvarez-Arguedas S, Castiella T, Vázquez FJ, Romero A, Zaragoza P, Martín-Burriel I, Rodellar C. (2012). Expansion under hypoxic conditions enhances the chondrogenic potential of equine bone marrow-derived mesenchymal stem cells. Vet J, 195(2), 248-251. https://doi.org/10.1016/j.tvjl.2012.06.008

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 195
Issue: 2
Pages: 248-251
PII: S1090-0233(12)00240-7

Researcher Affiliations

Ranera, Beatriz
  • Laboratorio de Genética Bioquímica LAGENBIO, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain. branera@unizar.es
Remacha, Ana Rosa
    Álvarez-Arguedas, Samuel
      Castiella, Tomás
        Vázquez, Francisco José
          Romero, Antonio
            Zaragoza, Pilar
              Martín-Burriel, Inmaculada
                Rodellar, Clementina

                  MeSH Terms

                  • Animals
                  • Bone Marrow / metabolism
                  • Cell Culture Techniques
                  • Chondrogenesis / physiology
                  • Gene Expression Regulation / drug effects
                  • Gene Expression Regulation / physiology
                  • Horses
                  • Mesenchymal Stem Cells / drug effects
                  • Mesenchymal Stem Cells / physiology
                  • Oxygen / pharmacology

                  Citations

                  This article has been cited 15 times.
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