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Home / Equine Research Bank / J Vet Intern Med / Experimental transmission of Corynebacterium pseudotuberculo...
Journal of veterinary internal medicine2015; 29(2); 636-643; doi: 10.1111/jvim.12545

Experimental transmission of Corynebacterium pseudotuberculosis biovar equi in horses by house flies.

Abstract: The route of Corynebacterium pseudotuberculosis infection in horses remains undetermined, but transmission by insects is suspected. Objective: To investigate house flies (Musca domestica L.) as vectors of C. pseudotuberculosis transmission in horses. Methods: Eight healthy, adult ponies. Methods: Randomized, controlled, blinded prospective study. Ten wounds were created in the pectoral region where cages for flies were attached. Three ponies were directly inoculated with C. pseudotuberculosis. Four ponies were exposed for 24 hours to 20 hours C. pseudotuberculosis-inoculated flies. One negative control pony was exposed to noninoculated flies. Ponies were examined daily for swelling, heat, pain, and drainage at the inoculation site. Blood was collected weekly for CBC and biochemical analysis, and twice weekly for synergistic hemolysis inhibition titers. Results: Clinical signs of local infection and positive cultures were observed in 7/7 exposed ponies and were absent in the negative control. In exposed ponies, peak serologic titers (1:512 to 1:2,048) were obtained between days 17 and 21. Seroconversion was not observed in the negative control. Neutrophil counts were higher in the positive and fly-exposed groups than in the negative control (P = .002 and P = .005) on day 3 postinoculation. Serum amyloid A concentrations were higher in the positive control than in the negative control and fly-exposed ponies on days 3 (P < .0001) and 7 (P = .0004 and P = .0001). No differences were detected for other biochemical variables. Conclusions: House flies can serve as mechanical vectors of C. pseudotuberculosis and can transmit the bacterium to ponies.
Copyright © 2015 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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Publication Date: 2015-03-31 PubMed ID: 25818218PubMed Central: PMC4895518DOI: 10.1111/jvim.12545Google Scholar: Lookup
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  • Journal Article
  • Randomized Controlled Trial
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper investigates whether house flies can transmit Corynebacterium pseudotuberculosis, a bacteria causing infection, to horses. It concludes that indeed, house flies can serve as mechanical vectors for the bacterium and can transmit it to ponies.

Introduction and Objectives

  • The primary aim of this study was to determine if house flies (Musca domestica L.) can act as vectors for the transmission of Corynebacterium pseudotuberculosis in horses.

Methodology

  • The researchers conducted a randomized, controlled, blinded prospective study involving eight healthy adult ponies.
  • Ten wounds were created on each pony’s pectoral region where cages for flies were attached. Three ponies were directly inoculated with C. pseudotuberculosis while four ponies were exposed to flies that had been inoculated with the bacterium for 24 hours. A negative control pony was exposed to non-inoculated flies.

Examinations and Analysis

  • The ponies were daily examined for signs of infection like swelling, heat, pain, and drainage at the inoculation site.
  • Blood samples were collected weekly for Complete Blood Count (CBC) and biochemical analysis, and twice weekly for synergistic hemolysis inhibition titers which is a test to detect antibodies.

Results

  • Local infection signs and positive cultures were observed in all the seven ponies exposed to the bacterium, while these were absent in the negative control.
  • Peak serologic titers, indicating an immune response, ranged from 1:512 to 1:2,048 and were noticed between the 17th and 21st days in the exposed ponies. The negative control did not show any seroconversion – a clear indication that an infection had taken place.
  • The neutrophil counts, which are a type of white blood cell that fights infection, were higher in the positive control and the fly-exposed group than in the negative control.
  • The positive control had a higher concentration of serum amyloid A, a biomarker for inflammation, than the negative control and the fly-exposed ponies, especially on the 3rd and 7th days after inoculation.

Conclusion

  • The results of this study demonstrate that house flies can act as mechanical vectors for C. pseudotuberculosis and transmit the bacterium to ponies, thereby causing infection.

Cite This Article

APA
Barba M, Stewart AJ, Passler T, Wooldridge AA, van Santen E, Chamorro MF, Cattley RC, Hathcock T, Hogsette JA, Hu XP. (2015). Experimental transmission of Corynebacterium pseudotuberculosis biovar equi in horses by house flies. J Vet Intern Med, 29(2), 636-643. https://doi.org/10.1111/jvim.12545

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 29
Issue: 2
Pages: 636-643

Researcher Affiliations

Barba, M
  • Department of Clinical Sciences, Auburn University, Auburn, AL.
Stewart, A J
    Passler, T
      Wooldridge, A A
        van Santen, E
          Chamorro, M F
            Cattley, R C
              Hathcock, T
                Hogsette, J A
                  Hu, X P

                    MeSH Terms

                    • Animals
                    • Corynebacterium Infections / microbiology
                    • Corynebacterium Infections / transmission
                    • Corynebacterium Infections / veterinary
                    • Corynebacterium pseudotuberculosis / physiology
                    • Diptera / microbiology
                    • Horse Diseases / microbiology
                    • Horse Diseases / transmission
                    • Horses
                    • Insect Vectors / microbiology
                    • Skin Diseases, Bacterial / microbiology
                    • Skin Diseases, Bacterial / transmission
                    • Skin Diseases, Bacterial / veterinary

                    References

                    This article includes 23 references
                    1. Quinn P, Markey B, Leonard F, et al. Veterinary Microbiology and Microbial Disease, 2nd ed Dublin: Wiley‐Blackwell; 2011.
                    2. Biberstein EL, Knight HD, Jang S. Two biotypes of Corynebacterium pseudotuberculosis. Vet Rec 1971;89:691–692.
                      pubmed: 5168555
                    3. Sutherland SS, Hart RA, Buller NB. Genetic differences between nitrate‐negative and nitrate‐positive C. pseudotuberculosis strains using restriction fragment length polymorphisms. Vet Microbiol 1996;49:1–9.
                      pubmed: 8861638
                    4. Yeruham I, Braverman Y, Shpigel NY. Mastitis in dairy cattle caused by Corynebacterium pseudotuberculosis and the feasibility of transmission by houseflies. Vet Quart 1996;18:87–89.
                      pubmed: 8903139
                    5. Aleman M, Spier S, Wilson WD. Corynebacterium pseudotuberculosis infection in horses: 538 cases (1982–1993). J Am Vet Med Assoc 1996;209:804–809.
                      pubmed: 8756884
                    6. Foley JE, Spier SJ, Mihalyi J. Molecular epidemiologic features of Corynebacterium pseudotuberculosis isolated from horses. Am J Vet Res 2004;65:1734–1737.
                      pubmed: 15631043
                    7. Dorella FA, Pacheco LGC, Oliveira SC. Corynebacterium pseudotuberculosis: Microbiology, biochemical properties, pathogenesis and molecular studies of virulence. Vet Res 2006;37:201–218.
                      pubmed: 16472520
                    8. Pratt SM, Spier SJ, Carroll SP. Evaluation of clinical characteristics, diagnostic test results, and outcome in horses with internal infection caused by Corynebacterium pseudotuberculosis: 30 cases (1995–2003). J Am Vet Med Assoc 2005;227:441–448.
                      pubmed: 16121612
                    9. . Pigeon fever detected in Florida. 2012.
                    10. Pratt SM, Spier SJ, Vaughan B. Respiratory disease caused by Corynebacterium pseudotuberculosis. Clin Tech Equine Pract 2006;5:239–246.
                    11. Miers KC, Ley WB. Corynebacterium pseudotuberculosis infection in the horse: Study of 117 clinical cases and consideration of etiopathogenesis. J Am Vet Med Assoc 1980;177:250–253.
                      pubmed: 6449498
                    12. Szonyi B, Swinford A, Clavijo A. Re‐emergence of pigeon fever (Corynebacterium pseudotuberculosis) infection in TEXAS horses: Epidemiologic investigation of laboratory‐diagnosed cases. J Equine Vet Sci 2014;34:281–287.
                    13. Kilcoyne I, Spier S, Carter C. Frequency of Corynebacterium pseudotuberculosis infection in horses across the United States during a 10‐year period. J Am Vet Med Assoc 2014;245:309–314.
                      pubmed: 25029310
                    14. Spier SJ, Leutenegger CM, Vet M. Use of a real‐time polymerase chain reaction‐based fluorogenic 5 ‘nuclease assay to evaluate insect vectors of Corynebacterium pseudotuberculosis infections in horses. Am J Vet Res 2002;65:829–834.
                      pubmed: 15198224
                    15. Braverman Y, Chizov‐Ginzburg A, Saran A. The role of houseflies (Musca domestica) in harbouring Corynebacterium pseudotuberculosis in dairy herds in Israel. Rev Sci Tech 1999;18:681–690.
                      pubmed: 10588012
                    16. Hogsette JA. New diets for production of house flies and stable flies (Diptera: Muscidae) in the laboratory. J Econ Entomol 1992;85:2291–2294.
                      pubmed: 1464690
                    17. Quinn G, Keough M. Experimental Design and Data Analysis for Biologists. Cambridge: Cambridge University Press; 2002.
                    18. Yozwiak ML, Songer JG. Effect of Corynebacterium pseudotuberculosis phospholipase D on viability and chemotactic responses of ovine neutrophils. Am J Vet Res 1993;54:392–397.
                      pubmed: 8498741
                    19. Nieto NC, Foley JE, Maclachlan NJ. Evaluation of hepatic disease in mice following intradermal inoculation with Corynebacterium pseudotuberculosis. Am J Vet Res 2009;70:257–262.
                      pubmed: 19231959
                    20. Britz E, Spier SJ, Kass PH. The relationship between Corynebacterium pseudotuberculosis biovar equi phenotype with location and extent of lesions in horses. Vet J 2014;200:282–286.
                      pubmed: 24703322
                    21. Eckersall PD, Lawson FP, Bence L. Acute phase protein response in an experimental model of ovine caseous lymphadenitis. BMC Vet Res 2007;3:35.
                      pmc: PMC2235841pubmed: 18093286
                    22. Jeske JM, Spier SJ, Whitcomb MB. Use of antibody titers measured via serum synergistic hemolysis inhibition testing to predict internal infection in horses. J Am Vet Med Assoc 2013;242:86–92.
                      pubmed: 23234286
                    23. Chamorro MF, Passler T, Givens MD. Evaluation of transmission of bovine viral diarrhea virus (BVDV) between persistently infected and naive cattle by the horn fly (Haematobia irritans). Vet Res Commun 2011;35:123–129.
                      pubmed: 21136161
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