Expression of 11β-HSD in steroid-induced avascular necrosis of the femoral head.
Abstract: The aim of the present study was to investigate the association between glucocorticoid receptors and steroid‑induced avascular necrosis of the femoral head (SANFH). Healthy New Zealand rabbits were randomly divided into 3 groups (n=16/group); the normal (no treatment), control (horse serum injections) and treatment (horse serum and methylprednisolone injections) groups. Methylprednisolone and horse serum injections were used to establish a SANFH model in rabbits; magnetic resonance imaging (MRI) and histopathological analysis were used to evaluate the SANFH rabbit model. Total cholesterol and triglyceride contents in the blood of SANFH rabbits were determined. The protein expression levels of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and type 2 (11β-HSD2) were determined using western blotting. The total cholesterol and triglyceride levels in the blood of methylprednisolone‑treated rabbits were significantly increased compared with the control and normal groups, which provides evidence in support of the metabolic disorder theory. Based on the results obtained from western blotting, the expression levels of 11β-HSD1 protein were increased, whereas the expression levels of 11β-HSD2 protein were decreased following injection. The results of the present study indicate that 11β-HSDs are important in the development of SANFH. Furthermore, 11β-HSDs may be important targets for preventing the development of ANFH in patients treated with steroids, which has a significant reference value for the use of steroids in clinical practice.
Publication Date: 2013-03-28 PubMed ID: 23546306DOI: 10.3892/mmr.2013.1401Google Scholar: Lookup
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- Journal Article
Summary
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This research investigates the link between glucocorticoid receptors and steroid-induced necrosis of the femoral head, a condition which cuts off blood supply to areas of bone tissue, causing it to die. The study makes use of rabbits to test this link, revealing how different steroids and proteins could contribute to the development of this condition.
Research Method
- The researchers selected healthy New Zealand rabbits for the experiment and divided them into three groups; the normal group, the control group, and the treatment group with each group comprising of sixteen rabbits. Each group received distinct treatments: the normal group received no treatment, the control group received horse serum injections, and the treatment group underwent both horse serum and methylprednisolone injections.
- The scientists induced SANFH in the rabbits using methylprednisolone and horse serum injections. They used magnetic resonance imaging (MRI) and histopathological analysis to validate the effectiveness of the SANFH model in the rabbits.
- The researchers also assessed the total cholesterol and triglyceride contents in the blood of the SANFH rabbits. These measurements help to provide evidence for the metabolic disorder theory, as the cholesterol and triglyceride levels significantly increased in the methylprednisolone-treated rabbits.
Key Findings
- The researchers used western blotting, a common method in molecular biology, to determine the protein expression levels of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and type 2 (11β-HSD2). These proteins are part of the family of enzymes that metabolize steroids. They found that the expression of 11β-HSD1 protein increased, and the expression of 11β-HSD2 protein decreased after injection, contributing to their affirmation of the role of these proteins in SANFH development.
- The key findings suggest that 11β-HSDs proteins are crucial to the development of steroid-induced avascular necrosis of the femoral head. These proteins may thus be crucial preventative targets in patients undergoing steroid treatments. This has significant implications for the administration of steroids in clinical practice.
Cite This Article
APA
Wang L, Luo DK, Pan ZY.
(2013).
Expression of 11β-HSD in steroid-induced avascular necrosis of the femoral head.
Mol Med Rep, 7(5), 1482-1486.
https://doi.org/10.3892/mmr.2013.1401 Publication
Researcher Affiliations
- Department of Orthopaedic Microsurgery and Hand Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, PR China.
MeSH Terms
- 11-beta-Hydroxysteroid Dehydrogenase Type 1 / metabolism
- Animals
- Blotting, Western
- Bone and Bones / enzymology
- Bone and Bones / pathology
- Cholesterol / blood
- Femur Head Necrosis / blood
- Femur Head Necrosis / chemically induced
- Femur Head Necrosis / enzymology
- Femur Head Necrosis / pathology
- Horses
- Magnetic Resonance Imaging
- Rabbits
- Steroids / adverse effects
- Triglycerides / blood
Citations
This article has been cited 8 times.- Xu H, Zeng Q, Zou K, Huang H, Chen J, Wang P, Yuan W, Xiao L, Tong P, Jin H. Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH.. Apoptosis 2023 Oct;28(9-10):1332-1345.
- Li Z, Shao W, Lv X, Wang B, Han L, Gong S, Wang P, Feng Y. Advances in experimental models of osteonecrosis of the femoral head.. J Orthop Translat 2023 Mar;39:88-99.
- Liu J, Li C, Yang F, Li M, Wu B, Chen H, Li S, Zhang X, Yang J, Xia Y, Wu M, Li Y, Liu B, Zhao D. Effects of angiotensin II combined with asparaginase and dexamethasone on the femoral head in mice: A model of steroid-induced femoral head osteonecrosis.. Front Cell Dev Biol 2022;10:975879.
- Liu D, Wang Y, Pan Z, Huang Z, Chen F. cAMP regulates 11β-hydroxysteroid dehydrogenase-2 and Sp1 expression in MLO-Y4/MC3T3-E1 cells.. Exp Ther Med 2020 Sep;20(3):2166-2172.
- Li F, Zhou C, Gao M, Xu L, Wen G, Zhao J, Yan J. Effect of 11β-HSD1 inhibitor on bone microstructure and bone density in rats with femoral head necrosis.. J Musculoskelet Neuronal Interact 2020 Jun 1;20(2):282-290.
- Li YH, Gao FQ, Cheng LM, Zeng MD, Qiu QY, Ding M. Different changes of microarchitectures of cortical and cancellous bones in sheep femoral head after long-term glucocorticoid interventions.. Sci Rep 2018 Jul 3;8(1):9988.
- Xu J, Gong H, Lu S, Deasey MJ, Cui Q. Animal models of steroid-induced osteonecrosis of the femoral head-a comprehensive research review up to 2018.. Int Orthop 2018 Jul;42(7):1729-1737.
- Zhang C, Zhu X, Pei G, Xu P, Zeng X, Zhang L, Zhang N, Zeng D, Cao L, Yu W, Zhang X. Dyslipidaemia for patients with low-energy femoral neck fractures after the treatment of cancellous screws: a retrospective study with a 3-year minimum follow-up.. BMC Musculoskelet Disord 2017 Nov 10;18(1):440.
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