Expression of angiogenic factors and luteinizing hormone receptors in the corpus luteum of mares induced to ovulate with deslorelin acetate.
Abstract: The effects of deslorelin acetate use in inducing ovulation need to be clarified to improve the results of equine embryo transfer. The mRNA abundance for angiogenic factors and LH receptor (LHR) in corpus luteum (CL) was studied in mares with natural (control group [CG]) and induced ovulation with deslorelin acetate (treatment group [TG]; follicles: ≥ 35 mm). Transrectal ultrasonography was used to verify the ovulation day, and on Days 4, 8, and 12 after ovulation (Day 0), CL samples were obtained through ultrasound-guided biopsy. The messenger RNA expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and LHR genes were analyzed by real-time polymerase chain reaction. A positive correlation was observed between VEGF and LHR (P < 0.00001, r = 0.78), and it was possible to detect higher LHR expression in the TG than in the CG on Day 4 (P < 0.05). Moreover, this expression was higher on Days 4 and 8 than on Day 12 in the TG. Basic fibroblast growth factor was also expressed in luteal tissue on all days for both groups; however, these differences were not significant. In conclusion, deslorelin acetate was effective for the induction of ovulation in mares, resulting in higher expression of LHR, especially on the fourth day after ovulation. In addition, VEGF expression was influenced by induced ovulation, with a lower level on Day 12, which is expected in nonpregnant mares.
Copyright © 2016 Elsevier Inc. All rights reserved.
Publication Date: 2015-09-18 PubMed ID: 26476595DOI: 10.1016/j.theriogenology.2015.09.025Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigates the changes in the expression of angiogenic factors and Luteinizing hormone receptors (LHR) in the corpus luteum of mares induced to ovulate with deslorelin acetate. The key finding of the research is that deslorelin acetate is effective for inducing ovulation and also resulted in higher expression of LHR in the early post-ovulation stage.
Research Methodology and Procedures
- The research was designed to study the mRNA abundance for angiogenic factors and LHR in mares naturally ovulated (Control Group) and those that were induced to ovulate with deslorelin acetate (Treatment Group).
- The study involved mares with 35mm or larger follicles with ovulation times verified through transrectal ultrasonography.
- Corpus luteum (CL) samples were extracted through ultrasound-guided biopsy on the 4th, 8th and 12th days after ovulation.
- Using a real-time polymerase chain reaction, the mRNA expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and LHR genes were analyzed.
Research Findings
- The research found a positive correlation between VEGF and LHR.
- It was observed that the expression of LHR was higher in the treatment group compared to the control group on the 4th day after ovulation.
- LHR expression was also higher on the 4th and 8th days post-ovulation, compared to the 12th day in the treatment group.
- The basic fibroblast growth factor was found present in the luteal tissue in both groups and on all days of the experiment, but the differences were not significant.
Conclusion
- The results of the research concluded that deslorelin acetate is effective in inducing ovulation for mares, and it leads to higher LHR expression especially on the fourth day after ovulation.
- The research also found that VEGF expression was influenced by induced ovulation, however, the level dropped by the 12th day which is expected in nonpregnant mares.
Cite This Article
APA
Maia VN, Batista AM, Cunha Neto S, Silva DM, Adrião M, Wischral A.
(2015).
Expression of angiogenic factors and luteinizing hormone receptors in the corpus luteum of mares induced to ovulate with deslorelin acetate.
Theriogenology, 85(3), 461-465.
https://doi.org/10.1016/j.theriogenology.2015.09.025 Publication
Researcher Affiliations
- Mauricio de Nassau University, Recife, Pernambuco, Brazil.
- Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
- Mauricio de Nassau University, Recife, Pernambuco, Brazil.
- Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
- Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
- Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil. Electronic address: aurea.wischral@gmail.com.
MeSH Terms
- Animals
- Corpus Luteum / metabolism
- Female
- Fibroblast Growth Factor 2 / genetics
- Gene Expression / drug effects
- Horses / physiology
- Ovulation / physiology
- Ovulation Induction / methods
- Ovulation Induction / veterinary
- RNA, Messenger / analysis
- Receptors, LH / genetics
- Triptorelin Pamoate / analogs & derivatives
- Triptorelin Pamoate / pharmacology
- Vascular Endothelial Growth Factor A / genetics
Citations
This article has been cited 1 times.- Billhaq DH, Lee S. The Role of the Guanosine Nucleotide-Binding Protein in the Corpus Luteum.. Animals (Basel) 2021 May 24;11(6).
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