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Theriogenology2013; 80(2); 145-152; doi: 10.1016/j.theriogenology.2013.03.025

Expression of enzymes and receptors of leukotriene pathway genes in equine endometrium during the estrous cycle and early pregnancy.

Abstract: The aims of the present study were to elucidate the expression profiles of leukotriene (LT) pathway mRNA transcription and to determine the possible interaction of LT and prostaglandin (PTG) pathways genes in equine endometrium during the estrous cycle and early pregnancy. Endometrial biopsies were obtained from mares on the day of ovulation (d0), at late diestrous (LD, n = 4), and after luteolysis in the estrus phase (AL, n = 4) of the cycle. Biopsies were also taken on Days 14 (P14; n = 4), 18 (P18, n = 4), and 22 (P22, n = 4) during early pregnancy that were comparable days to cyclic sampling days. A mixed model was fitted on the normalized relative mRNA levels, quantified by qPCR in duplicate, and least significant difference test was employed to detect significantly different group(s). In addition, to determine the degree of contribution of each gene to separation of treatment groups, the multivariate projection method partial least square regression discriminant analysis was used. The expression of 5-lipoxygenase mRNA was greater on d0 and LD, declined at AL, and was suppressed by early pregnancy. Leukotriene A4 hydrolase mRNA expression increased at LD and during early pregnancy, but was significantly greater at LD compared with P14. The expression of LT C4 synthase mRNA was only induced at LD. Cysteinyl leukotriene receptors (CysLT1 and CysLT2) mRNA expressions were decreased by both cyclic changes and early pregnancy, whereas 5-lipoxygenase-activating protein and B leukotriene receptor mRNA expressions were not affected by early pregnancy or stages of the estrous cycle. Partial least square discriminant analysis suggests that LT and PTG pathway enzymes and receptors appear to behave similarly in terms of mRNA expression. In conclusion, the expression profiles of LT pathway genes are demonstrated in equine endometrium for the first time by the present study, and the present data suggest that LT pathway mRNA transcriptions are tightly regulated during early pregnancy in mares.
Copyright © 2013 Elsevier Inc. All rights reserved.
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Publication Date: 2013-04-24 PubMed ID: 23623165DOI: 10.1016/j.theriogenology.2013.03.025Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates how leukotriene (LT) pathway genes’ expression changes in horse uterine tissue (endometrium) during various stages of the estrous cycle and early pregnancy. The goal is to comprehend the possible interaction of LT pathway genes with prostaglandin (PTG) pathway genes.

Objective and Methodology

  • The study aims to understand the expression profiles and interactions of LT and PTG pathway genes during the estrous cycle and early pregnancy in horses.
  • Uterine tissue samples were collected from female horses (mares) at various stages of the estrous cycle and early pregnancy.
  • The mRNA levels were quantified using a technique called qPCR. The measurement was made twice to ensure accuracy.
  • A statistical analysis was conducted to identify significant differences between groups.
  • A multivariate projection method, called partial least square regression discriminant analysis, was employed to determine each gene’s contribution to group differences.

Key Findings

  • Expression of 5-lipoxygenase mRNA was found to be higher at the start of the cycle (day 0) and in the late part of the estrous cycle. Its expression reduced after the luteolysis phase and during early pregnancy.
  • The expression of Leukotriene A4 hydrolase mRNA increased during late diestrus and early pregnancy, but was far greater at late diestrus than during early pregnancy (P14).
  • LT C4 synthase mRNA was only observed during late diestrus.
  • Expression of cysteinyl leukotriene receptors (CysLT1 and CysLT2) mRNA decreased due to cyclic changes and early pregnancy, while 5-lipoxygenase-activating protein and B leukotriene receptor mRNA expressions were not affected by either conditions.
  • The multivariate analysis showed that LT and PTG pathway enzymes and receptors tend to behave similarly in terms of mRNA expression.

Conclusion

  • The study is the first to provide a comprehensive depiction of the expression profiles of LT pathway genes in the equine endometrium.
  • The data implies that the transcriptions of LT pathway mRNA are tightly regulated during the early stages of pregnancy in mares.

Cite This Article

APA
Guzeloglu A, Atli MO, Kurar E, Kayis SA, Handler J, Semacan A, Aslan S. (2013). Expression of enzymes and receptors of leukotriene pathway genes in equine endometrium during the estrous cycle and early pregnancy. Theriogenology, 80(2), 145-152. https://doi.org/10.1016/j.theriogenology.2013.03.025

Publication

Theriogenology
ISSN: 1879-3231
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 80
Issue: 2
Pages: 145-152
PII: S0093-691X(13)00125-8

Researcher Affiliations

Guzeloglu, Aydin
  • Department of Genetics, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey.
Atli, Mehmet O
    Kurar, Ercan
      Kayis, Seyit A
        Handler, Johannes
          Semacan, Ahmet
            Aslan, Selim

              MeSH Terms

              • Animals
              • Arachidonic Acid / metabolism
              • Endometrium / metabolism
              • Enzymes / genetics
              • Enzymes / metabolism
              • Estrous Cycle / blood
              • Estrous Cycle / genetics
              • Female
              • Gestational Age
              • Horses / physiology
              • Leukotrienes / metabolism
              • Male
              • Pregnancy
              • Pregnancy, Animal / genetics
              • Receptors, Leukotriene / genetics
              • Receptors, Leukotriene / metabolism
              • Signal Transduction / genetics

              Citations

              This article has been cited 1 times.
              1. Jana B, Andronowska A, Całka J, Mówińska A. Biosynthetic pathway for leukotrienes is stimulated by lipopolysaccharide and cytokines in pig endometrial stromal cells. Sci Rep 2025 Jan 22;15(1):2806.
                doi: 10.1038/s41598-025-86787-1pubmed: 39843578google scholar: lookup
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