Expression of interleukin-1β, interleukin-8, and interferon-γ in blood samples obtained from healthy and sick neonatal foals.
Abstract: To evaluate and compare the gene expression of interleukin(IL)-1β, IL-8, and interferon-γ during the first 72 hours after birth in healthy foals and during the first 72 hours after hospitalization in sick neonatal foals and investigate correlations of clinicopathologic variables with cytokine expressions in healthy and sick neonatal foals. Methods: 33 foals < 7 days old (10 healthy foals, 7 foals with sepsis, 6 foals with peripartum asphyxia syndrome, and 12 foals with other diseases [2 with failure of passive transfer of immunity only were not further evaluated]). Methods: A blood sample (15 mL) was collected from each foal immediately after birth or hospital admission (0 hours) and at 24 and 72 hours later. Clinicopathologic variables were evaluated, and cytokine gene expression in WBCs was measured with an absolute quantitative real-time reverse transcriptase PCR assay. Results: At all time points, gene expression of interferon-γ was low in all groups. No time-dependent changes in cytokine expressions were detected in healthy or sick foals. Foals with sepsis had significantly higher IL-1β gene expression than did healthy foals, foals with peripartum asphyxia syndrome, or foals with other diseases. At 0 hours, IL-1β expression was correlated with plasma fibrinogen concentration in healthy foals and with the neutrophil-to-lymphocyte ratio in foals with sepsis; IL-8 expression was correlated with monocyte count in foals with sepsis and with arterial pH, plasma fibrinogen concentration, and plasma lactate concentration in foals with peripartum asphyxia syndrome. Conclusions: Data have suggested that evaluation of IL-1β expression in sick neonatal foals could help identify those with sepsis.
Publication Date: 2012-08-29 PubMed ID: 22924724DOI: 10.2460/ajvr.73.9.1418Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research looks into the gene expression of interleukin-1β, IL-8, and interferon-γ within the first 72 hours of birth in both healthy and sick newborn horses. It suggests that the evaluation of IL-1β expression in sick neonatal foals could help identify those with sepsis.
Objective of the Study
- The research was conducted to evaluate and compare the gene expression of interleukin-1β (IL-1β), IL-8, and interferon-γ during the first 72 hours after birth in both healthy and sick neonatal foals.
- The study also aimed to investigate possible correlations of clinicopathologic variables with cytokine expressions in these foals.
Methods Employed
- The study was conducted on 33 foals that were less than seven days old, including 10 healthy foals, 7 foals with sepsis, 6 foals with peripartum asphyxia syndrome, and 12 with other diseases.
- A 15mL blood sample was taken from each foal immediately after birth or hospital admission and again at 24 and 72 hours later.
- The clinicopathologic variables were evaluated, and cytokine gene expression in white blood cells (WBCs) was measured using an absolute quantitative real-time reverse transcriptase PCR assay.
Results of the Study
- Throughout all time points, the gene expression of interferon-γ was low in all groups, and no time-dependent changes in cytokine expressions were found in either healthy or sick foals.
- Foals with sepsis were found to have significantly higher IL-1β gene expression than healthy foals, foals with peripartum asphyxia syndrome, or foals with other diseases.
- The study also revealed correlations between IL-1β and other variables such as plasma fibrinogen concentration in healthy foals and the neutrophil-to-lymphocyte ratio in foals with sepsis. Correlations were also discovered between IL-8 expression and monocyte count in foals with sepsis, and arterial pH, plasma fibrinogen concentration, and plasma lactate concentration in foals with peripartum asphyxia syndrome.
Conclusions of the Study
- The data from this study suggested that evaluating IL-1β expression in sick neonatal foals could aid in identifying those with sepsis.
Cite This Article
APA
Castagnetti C, Mariella J, Pirrone A, Cinotti S, Mari G, Peli A.
(2012).
Expression of interleukin-1β, interleukin-8, and interferon-γ in blood samples obtained from healthy and sick neonatal foals.
Am J Vet Res, 73(9), 1418-1427.
https://doi.org/10.2460/ajvr.73.9.1418 Publication
Researcher Affiliations
- Department of Veterinary Medical Sciences, Faculty of Veterinary Medicine, University of Bologna, 40064 Ozzano Emilia, Bologna, Italy. carolina.castagnetti@unibo.it
MeSH Terms
- Animals
- Animals, Newborn
- Asphyxia / blood
- Asphyxia / genetics
- Asphyxia / immunology
- Asphyxia / veterinary
- Fibrinogen / immunology
- Fibrinogen / metabolism
- Horse Diseases / blood
- Horse Diseases / genetics
- Horse Diseases / immunology
- Horses
- Interferon-gamma / biosynthesis
- Interferon-gamma / genetics
- Interferon-gamma / immunology
- Interleukin-1beta / biosynthesis
- Interleukin-1beta / genetics
- Interleukin-1beta / immunology
- Interleukin-8 / biosynthesis
- Interleukin-8 / genetics
- Interleukin-8 / immunology
- Lactic Acid / blood
- Lactic Acid / immunology
- RNA / chemistry
- RNA / genetics
- Real-Time Polymerase Chain Reaction
- Sepsis / blood
- Sepsis / genetics
- Sepsis / immunology
- Sepsis / veterinary
- Statistics, Nonparametric
Citations
This article has been cited 13 times.- Hu D, Tang Y, Wang C, Qi Y, Ente M, Li X, Zhang D, Li K, Chu H. The Role of Intestinal Microbial Metabolites in the Immunity of Equine Animals Infected With Horse Botflies. Front Vet Sci 2022;9:832062.
- Taylor SD, Serpa PBS, Santos AP, Hart KA, Vaughn SA, Moore GE, Mukhopadhyay A, Page AE. Effects of intravenous administration of peripheral blood-derived mesenchymal stromal cells after infusion of lipopolysaccharide in horses. J Vet Intern Med 2022 Jul;36(4):1491-1501.
- Anderson MJ, Ibrahim AS, Cooper BR, Woolcock AD, Moore GE, Taylor SD. Effects of administration of ascorbic acid and low-dose hydrocortisone after infusion of sublethal doses of lipopolysaccharide to horses. J Vet Intern Med 2020 Nov;34(6):2710-2718.
- Mendoza Garcia FJ, Gonzalez-De Cara C, Aguilera-Aguilera R, Buzon-Cuevas A, Perez-Ecija A. Meloxicam ameliorates the systemic inflammatory response syndrome associated with experimentally induced endotoxemia in adult donkeys. J Vet Intern Med 2020 Jul;34(4):1631-1641.
- Taylor S. A review of equine sepsis. Equine Vet Educ 2015 Feb;27(2):99-109.
- Merlo B, Teti G, Lanci A, Burk J, Mazzotti E, Falconi M, Iacono E. Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells. BMC Vet Res 2019 Aug 2;15(1):277.
- Sheats MK. A Comparative Review of Equine SIRS, Sepsis, and Neutrophils. Front Vet Sci 2019;6:69.
- Martin EM, Messenger KM, Sheats MK, Jones SL. Misoprostol Inhibits Lipopolysaccharide-Induced Pro-inflammatory Cytokine Production by Equine Leukocytes. Front Vet Sci 2017;4:160.
- Vinther AM, Heegaard PM, Skovgaard K, Buhl R, Andreassen SM, Andersen PH. Characterization and differentiation of equine experimental local and early systemic inflammation by expression responses of inflammation-related genes in peripheral blood leukocytes. BMC Vet Res 2016 Jun 1;12:83.
- Vinther AM, Skovgaard K, Heegaard PM, Andersen PH. Dynamic expression of leukocyte innate immune genes in whole blood from horses with lipopolysaccharide-induced acute systemic inflammation. BMC Vet Res 2015 Jun 16;11:134.
- Davias C, Mendoza FJ, De Las Heras A, Gonzalez-De-Cara C, Buzon-Cuevas A, Perez-Ecija A. Hematologic Ratios in Donkeys: Reference Intervals and Response to Experimentally Induced Endotoxemia. Animals (Basel) 2025 Aug 4;15(15).
- Hobbs KJ, Bayless R, Sheats MK. A Comparative Review of Cytokines and Cytokine Targeting in Sepsis: From Humans to Horses. Cells 2024 Sep 5;13(17).
- Samuels AN, Kamr AM, Reed SM, Slovis NM, Hostnik LD, Burns TA, Toribio RE. Association of the neutrophil-lymphocyte ratio with outcome in sick hospitalized neonatal foals. J Vet Intern Med 2024 Mar-Apr;38(2):1196-1206.
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