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Home / Equine Research Bank / PLoS One / Expression of microRNAs in Horse Plasma and Their Characteri...
PloS one2016; 11(1); e0146374; doi: 10.1371/journal.pone.0146374

Expression of microRNAs in Horse Plasma and Their Characteristic Nucleotide Composition.

Abstract: MicroRNAs (miRNAs) in blood plasma are stable under high levels of ribonuclease activity and could function in tissue-to-tissue communication, suggesting that they may have distinctive structural characteristics compared with non-circulating miRNAs. In this study, the expression of miRNAs in horse plasma and their characteristic nucleotide composition were examined and compared with non-plasma miRNAs. Highly expressed plasma miRNA species were not part of the abundant group of miRNAs in non-plasma tissues, except for the eca-let-7 family. eca-miR-486-5p, -92a, and -21 were among the most abundant plasma miRNAs, and their human orthologs also belong to the most abundant group of miRNAs in human plasma. Uracil and guanine were the most common nucleotides of both plasma and non-plasma miRNAs. Cytosine was the least common in plasma and non-plasma miRNAs, although levels were higher in plasma miRNAs. Plasma miRNAs also showed higher expression levels of miRNAs containing adenine and cytosine repeats, compared with non-plasma miRNAs. These observations indicate that miRNAs in the plasma have a unique nucleotide composition.
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Publication Date: 2016-01-05 PubMed ID: 26731407PubMed Central: PMC4711666DOI: 10.1371/journal.pone.0146374Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the investigation conducted to understand the expression of microRNAs (miRNAs) in horse plasma, their characteristic nucleotide composition, and how they compare with non-plasma miRNAs.

Research Purpose

  • The study aims to discern the structural characteristics, expression, and nucleotide composition of microRNAs (miRNAs) present in horse plasma.
  • It further seeks to contrast these features with non-circulating miRNAs observed in non-plasma tissues.

Key Findings

  • The study revealed that the most expressed miRNAs in plasma are not the ones found to be abundant in non-plasma tissues, except for the eca-let-7 family.
  • Among the most prevalent plasma miRNAs are eca-miR-486-5p, -92a, and -21. These miRNAs’ human counterparts are also found in abundance in human plasma, indicating a certain pattern or similarity across species.
  • Uracil and guanine emerged as the most frequent nucleotides in both, plasma as well as non-plasma miRNAs. However, cytosine, despite being the least common nucleotide in both categories, was found in greater amounts in plasma miRNAs compared to non-plasma miRNAs.
  • Plasma miRNAs also showed a higher expression of miRNAs containing adenine and cytosine repeats, in contrast to their non-plasma counterparts.

Significance of the Research Study

  • This study presents key insights into the unique structure and composition of miRNAs existing in plasma, which are known for their stability under high ribonuclease activity and potential role in tissue-to-tissue communication.
  • Unveiling the dissimilarity in nucleotide composition and expression of miRNAs in plasma and non-plasma tissues could further aid in understanding their distinct functionalities and potential involvement in various biological processes.
  • The research could lay the foundation for future studies focused on understanding the role and implications of these structural differences, potentially contributing to further advancements in the field of genomics and biomedicine.

Cite This Article

APA
Lee S, Hwang S, Yu HJ, Oh D, Choi YJ, Kim MC, Kim Y, Ryu DY. (2016). Expression of microRNAs in Horse Plasma and Their Characteristic Nucleotide Composition. PLoS One, 11(1), e0146374. https://doi.org/10.1371/journal.pone.0146374

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 11
Issue: 1
Pages: e0146374
PII: e0146374

Researcher Affiliations

Lee, Seungwoo
  • BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
Hwang, Seungwoo
  • Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, South Korea.
Yu, Hee Jeong
  • BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
Oh, Dayoung
  • BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
Choi, Yu Jung
  • BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
Kim, Myung-Chul
  • BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
Kim, Yongbaek
  • BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
Ryu, Doug-Young
  • BK21 Plus Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.

MeSH Terms

  • Animals
  • Horses
  • MicroRNAs / blood
  • MicroRNAs / metabolism
  • Nucleotides / blood
  • Nucleotides / metabolism

Conflict of Interest Statement

The authors have declared that no competing interests exist.

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This article has been cited 13 times.
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