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Home / Equine Research Bank / Animals (Basel) / Expression of Phosphatonin-Related Genes in Sheep, Dog and H...
Animals : an open access journal from MDPI2020; 10(10); 1806; doi: 10.3390/ani10101806

Expression of Phosphatonin-Related Genes in Sheep, Dog and Horse Kidneys Using Quantitative Reverse Transcriptase PCR.

Abstract: The aim of this preliminary study was to determine the relative expression of phosphatonin pathway-related genes in normal dog, sheep and horse kidneys and to explore the relationships between the different genes. Kidneys were collected post-mortem from 10 sheep, 10 horses and 8 dogs. RNA was extracted, followed by reverse transcriptase quantitative polymerase chain reaction for fibroblast growth factor receptor 1 IIIc (), sodium-phosphate co-transporter () 1 (), (), (), parathyroid hormone 1 receptor (), klotho (), vitamin D receptor (), 1a-hydroxylase () and 24-hydroxylase (). was highly expressed in the dog kidneys, compared with those of the horses and sheep. had greatest expression in horses and sheep, although the three different NPTs all had relatively similar expression in sheep. There was little variability in expression, particularly in the dogs and horses. expression was negatively correlated with genes (except in sheep), while genes were all positively correlated with each other. Unexpectedly, klotho was positively correlated with genes in all three species. These results provide the basis for further research into this important regulatory system. In particular, species differences in phosphatonin gene expression should be considered when considering the pathogenesis of chronic kidney disease.
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Publication Date: 2020-10-05 PubMed ID: 33027890PubMed Central: PMC7601102DOI: 10.3390/ani10101806Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the expression levels of genes related to phosphatonin pathways in the kidneys of dogs, sheep, and horses, and discusses the correlations among these genes. It provides the foundation for more detailed research into this regulatory system.

Research Background and Objectives

  • Phosphatonins are a group of substances that help regulate phosphate in the body. They play a key role in controlling kidney function and can be implicated in chronic kidney disease.
  • The study aimed to analyze the relative gene expression of various factors associated with phosphatonin pathways in the kidneys of three different species – dogs, sheep, and horses.
  • This preliminary exploration serves as a foundation for further research into this critical regulatory system, particularly with respect to the pathogenesis of chronic kidney disease and the species differences of gene expression.

Methodology

  • The researchers collected kidneys post-mortem from 10 sheep, 10 horses, and 8 dogs.
  • RNA was extracted from the kidneys, a process necessary for studying gene expression, and used to conduct a reverse transcriptase quantitative polymerase chain reaction (RT-qPCR).
  • This method was applied to particular genes related to the phosphatonin pathway namely: fibroblast growth factor receptor 1 IIIc, sodium-phosphate co-transporter 1, parathyroid hormone 1 receptor, klotho, vitamin D receptor, 1a-hydroxylase, and 24-hydroxylase.

Findings

  • The fibroblast growth factor receptor 1 IIIc gene showed the highest expression in the kidneys of dogs compared to the kidneys of horses and sheep. The sodium-phosphate co-transporter 1 genes exhibited greater expression in horses and sheep but had similar expression levels among different types in sheep.
  • Expression of the parathyroid hormone 1 receptor gene showed little variation, specifically in dogs and horses.
  • The study revealed certain correlations between the gene expressions. The expression of the vitamin D receptor gene was negatively correlated with that of sodium-phosphate co-transporter genes in sheep. However, sodium-phosphate co-transporter genes expressed themselves positively correlatively.
  • Surprisingly, klotho, a gene that influences aging, exhibited a positive correlation with sodium-phosphate co-transporter gene expressions in all three species.

Implications and Conclusion

  • The study’s findings serve as the groundwork for expanding the understanding of the phosphatonin regulatory system. It provided insights into the expression of genes involved in this system in three species.
  • The variations in gene expressions between species emphasize the need to account for these differences when studying the pathogenesis of chronic kidney disease.

Cite This Article

APA
Dittmer KE, Heathcott RW, Marshall JC, Azarpeykan S. (2020). Expression of Phosphatonin-Related Genes in Sheep, Dog and Horse Kidneys Using Quantitative Reverse Transcriptase PCR. Animals (Basel), 10(10), 1806. https://doi.org/10.3390/ani10101806

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 10
Issue: 10
PII: 1806

Researcher Affiliations

Dittmer, Keren E
  • School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.
Heathcott, Rosemary W
  • School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.
Marshall, Jonathan C
  • School of Fundamental Sciences, Massey University, Palmerston North 4410, New Zealand.
Azarpeykan, Sara
  • School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand.

Grant Funding

  • McGeorge Fund / Institute of Veterinary, Animal and Biomedical Sciences, Massey University

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Citations

This article has been cited 1 times.
  1. Dittmer KE, Chernyavtseva A, Marshall JC, Cabrera D, Wolber FM, Kruger M. Expression of Renal Vitamin D and Phosphatonin-Related Genes in a Sheep Model of Osteoporosis.. Animals (Basel) 2021 Dec 29;12(1).
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