Expression of transforming growth factor-beta 1 in normal and dyschondroplastic articular growth cartilage of the young horse.
Abstract: This study describes the distribution pattern of transforming growth factor-beta 1 (TGF-beta 1) mRNA and protein in normal pre- and post natal growth cartilage and alterations present in lesions of dyschondroplasia (osteochondrosis). TGF-beta 1 expression and immunoreactivity have been investigated by in situ hybridisation and immunolocalisation in the articular/epiphyseal growth cartilage of the lateral trochlear ridge of the distal femur. Cartilage was obtained from 19 normal Thoroughbred horses (5 prenatal and 14 post natal horses) and 15 post natal horses with dyschondroplasia (DCP). TGF-beta 1 mRNA expression and immunoreactivity were detected in the proliferative and upper hypertrophic zones in both pre- and post natal normal articular/epiphyseal cartilage. However, mRNA itself was only detected in the mid- and lower hypertrophic zones. Immunoreactivity was identified intracellularly with some nuclear staining observed. In focal lesions of DCP mRNA expression and immunoreactivity were reduced compared to normal cartilage, but strong mRNA expression was observed in the chondrocyte clusters immediately surrounding a lesion of DCP. The results described in this study demonstrate alterations in TGF-beta 1 dyschondroplastic lesions and indicate that it could be involved in the pathogenesis of this condition in the horse.
Publication Date: 1997-12-31 PubMed ID: 9413715DOI: 10.1111/j.2042-3306.1997.tb03155.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research explores the pattern of transforming growth factor-beta 1 (TGF-beta 1) in normal and abnormal growth cartilage in horses, both before and after birth, and how this might relate to a condition known as dyschondroplasia.
Research Methodology
- The researchers used a combination of in-situ hybridisation (a method to detect specific RNA sequences) and immunolocalisation (a way to locate proteins in cells) to determine TGF-beta 1 expression and its locations in the cartilage of horse’s femur.
- The cartilage was collected from two different groups of horses; 19 normal ones (including 5 prenatal and 14 post-natal) and 15 with dyschondroplasia.
Research Findings
- In both groups (both pre- and post-natal), TGF-beta 1 mRNA expression was detected in the proliferative and upper hypertrophic zones. mRNA expression refers to the process by which information from a gene is utilized to create a functional product, in this case, the TGF-beta 1 protein.
- The mRNA was only detected in the mid- and lower hypertrophic zones of the cartilage. A hypertrophic zone refers to the area within growth plates that contains enlarged, or “hypertrophic,” cartilage cells and is a critical area for bone growth.
- Immunoreactivity was identified intracellularly, meaning within the cells, and there was some staining observed in the nucleus of the cells. This suggests that the TGF-beta 1 protein was being actively produced in these areas.
- In the horses with dyschondroplasia, both mRNA expression and immunoreactivity were reduced compared to the normal horses, indicating a lower level of TGF-beta 1 protein production. However, strong mRNA expression was observed in the areas surrounding the dyschondroplasia lesions, suggesting an active response to the condition.
Conclusions
- The results from the research suggest alterations in TGF-beta 1 in dyschondroplastic lesions, indicating a potential role of TGF-beta 1 in the development of dyschondroplasia. This discovery might offer valuable insights for understanding and potentially treating this condition in horses.
Cite This Article
APA
Henson FM, Schofield PN, Jeffcott LB.
(1997).
Expression of transforming growth factor-beta 1 in normal and dyschondroplastic articular growth cartilage of the young horse.
Equine Vet J, 29(6), 434-439.
https://doi.org/10.1111/j.2042-3306.1997.tb03155.x Publication
Researcher Affiliations
- Department of Clinical Veterinary Medicine, University of Cambridge, UK.
MeSH Terms
- Animals
- Blotting, Northern / veterinary
- Cartilage, Articular / chemistry
- Cartilage, Articular / metabolism
- Cartilage, Articular / pathology
- Cross Reactions
- DNA, Complementary / analysis
- DNA, Complementary / chemistry
- DNA, Complementary / genetics
- Gene Expression Regulation
- Horse Diseases / genetics
- Horse Diseases / metabolism
- Horse Diseases / pathology
- Horses
- Immune Sera / immunology
- Immunohistochemistry
- In Situ Hybridization / methods
- In Situ Hybridization / veterinary
- Osteochondrodysplasias / metabolism
- Osteochondrodysplasias / pathology
- Osteochondrodysplasias / veterinary
- RNA, Messenger / analysis
- RNA, Messenger / chemistry
- RNA, Messenger / genetics
- Transforming Growth Factor beta / analysis
- Transforming Growth Factor beta / genetics
- Transforming Growth Factor beta / immunology
Citations
This article has been cited 1 times.- Kemper AM, Drnevich J, McCue ME, McCoy AM. Differential Gene Expression in Articular Cartilage and Subchondral Bone of Neonatal and Adult Horses. Genes (Basel) 2019 Sep 25;10(10).
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