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Home / Equine Research Bank / BMC Vet Res / Expression patterns of intestinal calcium transport factors ...
BMC veterinary research2011; 7; 65; doi: 10.1186/1746-6148-7-65

Expression patterns of intestinal calcium transport factors and ex-vivo absorption of calcium in horses.

Abstract: In many species, the small intestine is the major site of calcium (Ca(2+)) absorption. The horse differs considerably from most other species with regard to the physiology of its Ca(2+) metabolism and digestion. Thus, this study was performed to get more information about the transcellular Ca(2+) absorption in the horse.Two mechanisms of intestinal Ca(2+) absorption are described: the passive paracellular pathway and the active, vitamin D-dependent transcellular pathway. The latter involves the following elements: vitamin D receptors (VDR), transient receptor potential vanilloid channel members 5 and 6 (TRPV5/6), calbindin-D9k (CB), the Na/Ca exchanger (NCX1) and the plasma membrane Ca-ATPase (PMCA). The aim of the present study was to investigate the protein and mRNA expression patterns of VDR, CB and TRPV6 and the ex-vivo Ca(2+) absorption in horses, assessed by qualitative and quantitative RT-PCR, western blot, immunohistochemistry and the Ussing chamber technique. Results: Highest CB and TRPV6 mRNA levels were detected in the duodenum as compared to the middle parts of the jejunum and ileum and several sites of the large intestine. VDR mRNA levels did not change significantly throughout the intestine. TRPV5 mRNA was not detectable in the horse intestine. The highest VDR and CB protein levels were measured in the duodenum. Ussing chamber studies revealed ex-vivo Ca(2+) absorption only in the duodenum, but not in cecum and specific sites of the colon. Conclusions: The present findings suggest that TRPV6, CB and VDR may be involved in active intestinal Ca(2+) absorption in horses, as described for other mammals. TRPV5 may not play a major role in this process. Furthermore, the expression patterns of these Ca(2+) transport elements and the results of the Ussing chamber procedure indicate that a significant part of active intestinal Ca(2+) absorption occurs in the duodenum in this species.
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Publication Date: 2011-10-22 PubMed ID: 22017756PubMed Central: PMC3221622DOI: 10.1186/1746-6148-7-65Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article looks into the intestinal calcium absorption in horses, studying how key elements related to this process such as vitamin D receptors (VDR), Calbindin-D9k (CB), and TRPV6 channel factor, are expressed in the horse’s body. The main finding suggests the majority of active calcium absorption occurs in the duodenum.

Study Approach and Elements

  • The research was aimed at understanding how calcium (Ca(2+)) is absorbed in the intestines of horses. It’s important to note that compared to many other species, horse physiology pertaining to calcium metabolism and digestion significantly varies.
  • Two primary mechanisms of calcium absorption are identified: the passive paracellular pathway and the active, vitamin D-dependent transcellular pathway. The latter involves multiple elements such as vitamin D receptors (VDR), transient receptor potential vanilloid channel members 5 and 6 (TRPV5/6), calbindin-D9k (CB), the Na/Ca exchanger (NCX1) and the plasma membrane Ca-ATPase (PMCA).
  • The research aimed to investigate the protein and mRNA expression patterns of VDR, CB and TRPV6 as they are the key players in the active, vitamin D-dependent transcellular pathway of calcium absorption.

Research Method and Results

  • The expression of these factors was scrutinized using various investigative techniques including qualitative and quantitative RT-PCR, western blot, immunohistochemistry and the Ussing chamber technique for assessing ex-vivo calcium absorption.
  • The results revealed that the highest levels of CB and TRPV6 mRNA were found in the duodenum, compared to the middle parts of the jejunum and ileum and several sites of the large intestine, indicating that duodenum primarily absorbs calcium in the horse’s body.
  • It was noted that VDR mRNA levels were almost consistent throughout the intestine. And TRPV5 mRNA was not detectable in the horse intestine indicating that it may not play a major role in calcium absorption.
  • Further, Ussing chamber studies have shown that ex-vivo calcium absorption took place only in the duodenum, not in other parts like the cecum and specific sites of the colon.

Conclusions of the Study

  • The study concluded that TRPV6, CB, and VDR could be integral in facilitating active intestinal calcium absorption as seen in other mammals, with TRPV5 possibly not playing a substantial role. Additionally, the expression patterns of these calcium transport elements and the results from the Ussing chamber technique suggest that the duodenum is a major site for active intestinal calcium absorption in horses.

Cite This Article

APA
Sprekeler N, Müller T, Kowalewski MP, Liesegang A, Boos A. (2011). Expression patterns of intestinal calcium transport factors and ex-vivo absorption of calcium in horses. BMC Vet Res, 7, 65. https://doi.org/10.1186/1746-6148-7-65

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 7
Pages: 65

Researcher Affiliations

Sprekeler, Nele
  • Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland. nelesprekeler@gmx.de
Müller, Tobias
    Kowalewski, Mariusz P
      Liesegang, Annette
        Boos, Alois

          MeSH Terms

          • Animals
          • Blotting, Western / veterinary
          • Calbindins
          • Calcium / metabolism
          • Female
          • Horses / metabolism
          • Ileum / metabolism
          • Intestinal Absorption
          • Intestine, Large / metabolism
          • Jejunum / metabolism
          • Male
          • Plasma Membrane Calcium-Transporting ATPases / metabolism
          • Real-Time Polymerase Chain Reaction / veterinary
          • Receptors, Calcitriol / metabolism
          • S100 Calcium Binding Protein G / metabolism
          • TRPV Cation Channels / metabolism

          References

          This article includes 41 references
          1. de Groot T, Bindels RJ, Hoenderop JG. TRPV5: an ingeniously controlled calcium channel.. Kidney Int 2008 Nov;74(10):1241-6.
            doi: 10.1038/ki.2008.320pubmed: 18596722google scholar: lookup
          2. Bronner F, Pansu D, Stein WD. An analysis of intestinal calcium transport across the rat intestine.. Am J Physiol 1986 May;250(5 Pt 1):G561-9.
            pubmed: 2939728doi: 10.1152/ajpgi.1986.250.5.g561google scholar: lookup
          3. van Abel M, Hoenderop JG, van der Kemp AW, van Leeuwen JP, Bindels RJ. Regulation of the epithelial Ca2+ channels in small intestine as studied by quantitative mRNA detection.. Am J Physiol Gastrointest Liver Physiol 2003 Jul;285(1):G78-85.
            pubmed: 12620887doi: 10.1152/ajpgi.00036.2003google scholar: lookup
          4. Bronner F, Pansu D. Nutritional aspects of calcium absorption.. J Nutr 1999 Jan;129(1):9-12.
            pubmed: 9915868doi: 10.1093/jn/129.1.9google scholar: lookup
          5. Eckermann-Ross C. Hormonal regulation and calcium metabolism in the rabbit.. Vet Clin North Am Exot Anim Pract 2008 Jan;11(1):139-52, vii.
            doi: 10.1016/j.cvex.2007.09.002pubmed: 18165142google scholar: lookup
          6. Peng JB, Chen XZ, Berger UV, Vassilev PM, Tsukaguchi H, Brown EM, Hediger MA. Molecular cloning and characterization of a channel-like transporter mediating intestinal calcium absorption.. J Biol Chem 1999 Aug 6;274(32):22739-46.
            doi: 10.1074/jbc.274.32.22739pubmed: 10428857google scholar: lookup
          7. Hoenderop JG, Vennekens R, Müller D, Prenen J, Droogmans G, Bindels RJ, Nilius B. Function and expression of the epithelial Ca(2+) channel family: comparison of mammalian ECaC1 and 2.. J Physiol 2001 Dec 15;537(Pt 3):747-61.
            pmc: PMC2278984pubmed: 11744752doi: 10.1111/j.1469-7793.2001.00747.xgoogle scholar: lookup
          8. Feher JJ, Fullmer CS, Wasserman RH. Role of facilitated diffusion of calcium by calbindin in intestinal calcium absorption.. Am J Physiol 1992 Feb;262(2 Pt 1):C517-26.
            pubmed: 1539638doi: 10.1152/ajpcell.1992.262.2.c517google scholar: lookup
          9. Bronner F. Intestinal calcium absorption: mechanisms and applications.. J Nutr 1987 Aug;117(8):1347-52.
            pubmed: 3305814doi: 10.1093/jn/117.8.1347google scholar: lookup
          10. Carafoli E. Calcium pump of the plasma membrane.. Physiol Rev 1991 Jan;71(1):129-53.
            pubmed: 1986387doi: 10.1152/physrev.1991.71.1.129google scholar: lookup
          11. Wasserman RH, Fullmer CS. Vitamin D and intestinal calcium transport: facts, speculations and hypotheses.. J Nutr 1995 Jul;125(7 Suppl):1971S-1979S.
            pubmed: 7602379doi: 10.1093/jn/125.suppl_7.1971sgoogle scholar: lookup
          12. Horst RL, Goff JP, Reinhardt TA. Adapting to the transition between gestation and lactation: differences between rat, human and dairy cow.. J Mammary Gland Biol Neoplasia 2005 Apr;10(2):141-56.
            doi: 10.1007/s10911-005-5397-xpubmed: 16025221google scholar: lookup
          13. Li YC, Bolt MJ, Cao LP, Sitrin MD. Effects of vitamin D receptor inactivation on the expression of calbindins and calcium metabolism.. Am J Physiol Endocrinol Metab 2001 Sep;281(3):E558-64.
            pubmed: 11500311doi: 10.1152/ajpendo.2001.281.3.e558google scholar: lookup
          14. Van Cromphaut SJ, Dewerchin M, Hoenderop JG, Stockmans I, Van Herck E, Kato S, Bindels RJ, Collen D, Carmeliet P, Bouillon R, Carmeliet G. Duodenal calcium absorption in vitamin D receptor-knockout mice: functional and molecular aspects.. Proc Natl Acad Sci U S A 2001 Nov 6;98(23):13324-9.
            doi: 10.1073/pnas.231474698pmc: PMC60869pubmed: 11687634google scholar: lookup
          15. Marcus CS, Lengemann FW. Absorption of Ca45 and Sr85 from solid and liquid food at various levels of the alimentary tract of the rat.. J Nutr 1962 Jun;77:155-60.
            pubmed: 14469699doi: 10.1093/jn/77.2.155google scholar: lookup
          16. CRAMER CF. SITES OF CALCIUM ABSORPTION AND THE CALCIUM CONCENTRATION OF GUT CONTENTS IN THE DOG.. Can J Physiol Pharmacol 1965 Jan;43:75-8.
            doi: 10.1139/y65-009pubmed: 14324234google scholar: lookup
          17. Wilkens MR, Kunert-Keil C, Brinkmeier H, Schröder B. Expression of calcium channel TRPV6 in ovine epithelial tissue.. Vet J 2009 Nov;182(2):294-300.
            doi: 10.1016/j.tvjl.2008.06.020pubmed: 18701326google scholar: lookup
          18. Schryver HF, Craig PH, Hintz HF, Hogue DE, Lowe JE. The site of calcium absorption in the horse.. J Nutr 1970 Oct;100(10):1127-31.
            pubmed: 5471046doi: 10.1093/jn/100.10.1127google scholar: lookup
          19. Rourke KM, Coe S, Kohn CW, Rosol TJ, Mendoza FJ, Toribio RE. Cloning, comparative sequence analysis and mRNA expression of calcium-transporting genes in horses.. Gen Comp Endocrinol 2010 May 15;167(1):6-10.
            doi: 10.1016/j.ygcen.2010.02.022pubmed: 20226785google scholar: lookup
          20. Ebel JG, Taylor AN, Wasserman RH. Vitamin D-induced calcium-binding protein of intestinal mucosa. Relation to vitamin D dose level and lag period.. Am J Clin Nutr 1969 Apr;22(4):431-6.
            pubmed: 4305086doi: 10.1093/ajcn/22.4.431google scholar: lookup
          21. Pérez AV, Picotto G, Carpentieri AR, Rivoira MA, Peralta López ME, Tolosa de Talamoni NG. Minireview on regulation of intestinal calcium absorption. Emphasis on molecular mechanisms of transcellular pathway.. Digestion 2008;77(1):22-34.
            doi: 10.1159/000116623pubmed: 18277073google scholar: lookup
          22. Schroeter-Vogt C. Immunhistochemische Untersuchung von Calbindin D9k im Darm von Kaninchen. Zürich: Universität Zürich; 2010.
          23. de Vries de Heekelingen T. Immunhistochemische Untersuchung zur Verteilung von Vitamin D-Rezeptoren im Darm von Kaninchen. Zürich: Universität Zürich; 2008.
          24. Toribio RE. Calcium Disorders. Equine Internal Medicine 2. St. Louis: Elsevier; 2004.
          25. Breidenbach A, Schlumbohm C, Harmeyer J. Peculiarities of vitamin D and of the calcium and phosphate homeostatic system in horses.. Vet Res 1998 Mar-Apr;29(2):173-86.
            pubmed: 9601149
          26. Liesegang A, Singer K, Boos A. Vitamin D receptor amounts across different segments of the gastrointestinal tract in Brown Swiss and Holstein Frisean cows of different age.. J Anim Physiol Anim Nutr (Berl) 2008 Jun;92(3):316-23.
            doi: 10.1111/j.1439-0396.2007.00782.xpubmed: 18477312google scholar: lookup
          27. GenBank. http://www.ncbi.nlm.nih.gov/genbank/
          28. Ishigaki Y, Li X, Serin G, Maquat LE. Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20.. Cell 2001 Sep 7;106(5):607-17.
            doi: 10.1016/S0092-8674(01)00475-5pubmed: 11551508google scholar: lookup
          29. USSING HH, ZERAHN K. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin.. Acta Physiol Scand 1951 Aug 25;23(2-3):110-27.
            doi: 10.1111/j.1748-1716.1951.tb00800.xpubmed: 14868510google scholar: lookup
          30. Höller H, Breves G, Kocabatmaz M, Gerdes H. Flux of calcium across the sheep rumen wall in vivo and in vitro.. Q J Exp Physiol 1988 Jul;73(4):609-18.
            pubmed: 2845464doi: 10.1113/expphysiol.1988.sp003180google scholar: lookup
          31. Lauff K. Einfluss von Calcium und Vitamin D auf den Knochenstoffwechsel und die Intensität der Vitamin D-Rezeptoren- sowie Calbindin D9k-Immunreaktionen im Gastrointestinaltrakt von Ziegenlämmern. Zürich: Universität Zürich; 2009.
          32. Sidler-Lauff K, Boos A, Kraenzlin M, Liesegang A. Influence of different calcium supplies and a single vitamin D injection on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of goat kids.. J Anim Sci 2010 Nov;88(11):3598-610.
            doi: 10.2527/jas.2009-2682pubmed: 20656967google scholar: lookup
          33. Schryver HF, Craig PH, Hintz HF. Calcium metabolism in ponies fed varying levels of calcium.. J Nutr 1970 Aug;100(8):955-64.
            pubmed: 5495848doi: 10.1093/jn/100.8.955google scholar: lookup
          34. Breves G, Schröder B. Vergleichende Aspekte der gastrointestinalen Calcium-Umsetzungen beim Schwein und Wiederkäuer. Lohmann Information 2005;1-3.
          35. Cehak A, Geburek F, Feige K, Schröder B, Breves G. Functional charakterisation of calcium and phosphate transport across the equine intestine. Proc Soc Nutr Physiol 2009.
          36. Wasserman RH, Fullmer CS. Calcium transport proteins, calcium absorption, and vitamin D.. Annu Rev Physiol 1983;45:375-90.
            doi: 10.1146/annurev.ph.45.030183.002111pubmed: 6221688google scholar: lookup
          37. Goff JP, Reinhardt TA, Horst RL. Milk fever and dietary cation-anion balance effects on concentration of vitamin D receptor in tissue of periparturient dairy cows.. J Dairy Sci 1995 Nov;78(11):2388-94.
            doi: 10.3168/jds.S0022-0302(95)76867-9pubmed: 8747330google scholar: lookup
          38. Liesegang A, Riner K, Boos A. Effects of gestation and lactation on vitamin D receptor amounts in goats and sheep.. Domest Anim Endocrinol 2007 Aug;33(2):190-202.
            doi: 10.1016/j.domaniend.2006.05.008pubmed: 16797913google scholar: lookup
          39. Boos A, Riner K, Hässig M, Liesegang A. Immunohistochemical demonstration of vitamin D receptor distribution in goat intestines.. Cells Tissues Organs 2007;186(2):121-8.
            doi: 10.1159/000102540pubmed: 17489022google scholar: lookup
          40. Mohri T, Nakajima M, Takagi S, Komagata S, Yokoi T. MicroRNA regulates human vitamin D receptor.. Int J Cancer 2009 Sep 15;125(6):1328-33.
            doi: 10.1002/ijc.24459pubmed: 19437538google scholar: lookup
          41. Hoenderop JG, Nilius B, Bindels RJ. Epithelial calcium channels: from identification to function and regulation.. Pflugers Arch 2003 Jun;446(3):304-8.
            pubmed: 12684797doi: 10.1007/s00424-003-1045-8google scholar: lookup

          Citations

          This article has been cited 2 times.
          1. Azarpeykan S, Dittmer KE, Marshall JC, Perera KC, Gee EK, Acke E, Thompson KG. Evaluation and Comparison of Vitamin D Responsive Gene Expression in Ovine, Canine and Equine Kidney. PLoS One 2016;11(9):e0162598.
            doi: 10.1371/journal.pone.0162598pubmed: 27632366google scholar: lookup
          2. Sprekeler N, Kowalewski MP, Boos A. TRPV6 and Calbindin-D9k-expression and localization in the bovine uterus and placenta during pregnancy. Reprod Biol Endocrinol 2012 Aug 29;10:66.
            doi: 10.1186/1477-7827-10-66pubmed: 22931437google scholar: lookup
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