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Veterinary sciences2024; 11(9); 427; doi: 10.3390/vetsci11090427

Extracted Plasma Cell-Free DNA Concentrations Are Elevated in Colic Patients with Systemic Inflammation.

Abstract: Colic is a common and potentially life-threatening condition in horses; in many cases, it remains challenging for clinicians to determine the cause, appropriate treatment, and prognosis. One approach that could improve patient care and outcomes is identification of novel diagnostic and prognostic biomarkers. Plasma cell-free DNA (cfDNA) is a biomarker that shows promise for characterizing disease severity and predicting survival in humans with acute abdominal pain or requiring emergency abdominal surgery. In horses, we recently determined that extracted plasma cfDNA concentrations are elevated in colic patients compared to healthy controls. For this current study, we hypothesized that extracted plasma cfDNA concentrations would be significantly higher in horses with strangulating or inflammatory colic lesions, in colic patients with systemic inflammatory response syndrome (SIRS), and in non-survivors. Cell-free DNA concentrations were measured in extracted plasma samples using a compact, portable Qubit fluorometer. Colic patients that met published criteria for equine SIRS had significantly higher median extracted plasma cfDNA compared to non-SIRS colic patients. There were no significant differences in extracted plasma cfDNA concentrations between other groups of interest. Our data offer early evidence that extracted plasma cfDNA concentration may provide information about systemic inflammation in colic patients, and additional research is warranted to expand on these findings.
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Publication Date: 2024-09-12 PubMed ID: 39330806PubMed Central: PMC11435807DOI: 10.3390/vetsci11090427Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the use of plasma cell-free DNA (cfDNA) as a marker to gauge the severity of colic, a common and critical condition in horses. The researchers found heightened concentrations of cfDNA in horses with colic and accompanying systemic inflammation, providing novel evidence that could enable more accurate diagnosis and prognosis of the condition.

Introduction and Hypothesis

  • The research is anchored on the potential utility of plasma cell-free DNA (cfDNA) as a diagnostic and prognostic biomarker for colic, a severe illness in horses that often presents diagnostic challenges.
  • The researchers had previously identified elevated cfDNA concentrations in horses suffering from colic compared to their healthy counterparts.
  • In this study, they hypothesized that horses with colic conditions that involve inflammation or strangulation, and those with systemic inflammatory response syndrome (SIRS), would exhibit significantly higher cfDNA concentrations. They also anticipated similar findings in non-surviving horses.

Methods

  • The researchers measured cfDNA concentrations in extracted plasma samples of the animals, utilizing a Qubit fluorometer, a compact device that allows for highly precise nucleic acid quantification.
  • They compared levels of cfDNA in horses diagnosed with colic that fulfilled established criteria for equine SIRS vs non-SIRS colic patients.

Findings

  • The results revealed remarkably greater median cfDNA concentrations in colic horses meeting SIRS criteria compared to those not presenting with SIRS.
  • However, there was no significant difference in cfDNA concentration between other subgroups of interest within the study.

Conclusion and Future Implications

  • The results suggest the potential of extracted plasma cfDNA concentrations as a key to understanding the level of systemic inflammation in colic patients.
  • While the findings offer initial evidence, further research is needed to extend and substantiate these observations. Such research could pave the way for improved clinical decision-making in treating horse colic, particularly in distinguishing between cases that necessitate surgical intervention and those that can be addressed non-surgically.

Cite This Article

APA
Bayless RL, Cooper BL, Sheats MK. (2024). Extracted Plasma Cell-Free DNA Concentrations Are Elevated in Colic Patients with Systemic Inflammation. Vet Sci, 11(9), 427. https://doi.org/10.3390/vetsci11090427

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 11
Issue: 9
PII: 427

Researcher Affiliations

Bayless, Rosemary L
  • Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
  • Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27607, USA.
Cooper, Bethanie L
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
  • Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27607, USA.
Sheats, M Katie
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
  • Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27607, USA.

Grant Funding

  • T32 OD011130 / NIH HHS
  • DoCS / North Carolina State University

Conflict of Interest Statement

The authors declare no conflicts of interest.

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Citations

This article has been cited 1 times.
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