FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Vet Sci / Extracted Plasma Cell-Free DNA Concentrations Are Elevated i...
Veterinary sciences2024; 11(9); 427; doi: 10.3390/vetsci11090427

Extracted Plasma Cell-Free DNA Concentrations Are Elevated in Colic Patients with Systemic Inflammation.

Abstract: Colic is a common and potentially life-threatening condition in horses; in many cases, it remains challenging for clinicians to determine the cause, appropriate treatment, and prognosis. One approach that could improve patient care and outcomes is identification of novel diagnostic and prognostic biomarkers. Plasma cell-free DNA (cfDNA) is a biomarker that shows promise for characterizing disease severity and predicting survival in humans with acute abdominal pain or requiring emergency abdominal surgery. In horses, we recently determined that extracted plasma cfDNA concentrations are elevated in colic patients compared to healthy controls. For this current study, we hypothesized that extracted plasma cfDNA concentrations would be significantly higher in horses with strangulating or inflammatory colic lesions, in colic patients with systemic inflammatory response syndrome (SIRS), and in non-survivors. Cell-free DNA concentrations were measured in extracted plasma samples using a compact, portable Qubit fluorometer. Colic patients that met published criteria for equine SIRS had significantly higher median extracted plasma cfDNA compared to non-SIRS colic patients. There were no significant differences in extracted plasma cfDNA concentrations between other groups of interest. Our data offer early evidence that extracted plasma cfDNA concentration may provide information about systemic inflammation in colic patients, and additional research is warranted to expand on these findings.
Get Full Text
Publication Date: 2024-09-12 PubMed ID: 39330806PubMed Central: PMC11435807DOI: 10.3390/vetsci11090427Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the use of plasma cell-free DNA (cfDNA) as a marker to gauge the severity of colic, a common and critical condition in horses. The researchers found heightened concentrations of cfDNA in horses with colic and accompanying systemic inflammation, providing novel evidence that could enable more accurate diagnosis and prognosis of the condition.

Introduction and Hypothesis

  • The research is anchored on the potential utility of plasma cell-free DNA (cfDNA) as a diagnostic and prognostic biomarker for colic, a severe illness in horses that often presents diagnostic challenges.
  • The researchers had previously identified elevated cfDNA concentrations in horses suffering from colic compared to their healthy counterparts.
  • In this study, they hypothesized that horses with colic conditions that involve inflammation or strangulation, and those with systemic inflammatory response syndrome (SIRS), would exhibit significantly higher cfDNA concentrations. They also anticipated similar findings in non-surviving horses.

Methods

  • The researchers measured cfDNA concentrations in extracted plasma samples of the animals, utilizing a Qubit fluorometer, a compact device that allows for highly precise nucleic acid quantification.
  • They compared levels of cfDNA in horses diagnosed with colic that fulfilled established criteria for equine SIRS vs non-SIRS colic patients.

Findings

  • The results revealed remarkably greater median cfDNA concentrations in colic horses meeting SIRS criteria compared to those not presenting with SIRS.
  • However, there was no significant difference in cfDNA concentration between other subgroups of interest within the study.

Conclusion and Future Implications

  • The results suggest the potential of extracted plasma cfDNA concentrations as a key to understanding the level of systemic inflammation in colic patients.
  • While the findings offer initial evidence, further research is needed to extend and substantiate these observations. Such research could pave the way for improved clinical decision-making in treating horse colic, particularly in distinguishing between cases that necessitate surgical intervention and those that can be addressed non-surgically.

Cite This Article

APA
Bayless RL, Cooper BL, Sheats MK. (2024). Extracted Plasma Cell-Free DNA Concentrations Are Elevated in Colic Patients with Systemic Inflammation. Vet Sci, 11(9), 427. https://doi.org/10.3390/vetsci11090427

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 11
Issue: 9
PII: 427

Researcher Affiliations

Bayless, Rosemary L
  • Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
  • Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27607, USA.
Cooper, Bethanie L
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
  • Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27607, USA.
Sheats, M Katie
  • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
  • Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27607, USA.

Grant Funding

  • T32 OD011130 / NIH HHS
  • DoCS / North Carolina State University

Conflict of Interest Statement

The authors declare no conflicts of interest.

References

This article includes 75 references
  1. Equine Mortality in the United States. Veterinary Services, Center for Epidemiology and Animal Health, Animal and Plant Inspection Service, United States Department of Agriculture; Fort Collins, CO, USA: 2017.
  2. Mair T.S., Smith L.J.. Survival and Complication Rates in 300 Horses Undergoing Surgical Treatment of Colic. Part 1: Short-Term Survival Following a Single Laparotomy.. Equine Vet. J. 2005;37:296–302.
    doi: 10.2746/0425164054529409pubmed: 16028616google scholar: lookup
  3. Christophersen M.T., Dupont N., Berg-Sørensen K.S., Konnerup C., Pihl T.H., Andersen P.H.. Short-Term Survival and Mortality Rates in a Retrospective Study of Colic in 1588 Danish Horses.. Acta Vet. Scand. 2014;56:20.
    doi: 10.1186/1751-0147-56-20pmc: PMC3998380pubmed: 24712831google scholar: lookup
  4. Mair T., Smith L.. Survival and Complication Rates in 300 Horses Undergoing Surgical Treatment of Colic. Part 4: Early (Acute) Relaparotomy.. Equine Vet. J. 2005;37:315–318.
    doi: 10.2746/0425164054529454pubmed: 16028619google scholar: lookup
  5. Reeves M.J., Curtis C.R., Salman M.D., Stashak T.S., Reif J.S.. Multivariable Prediction Model for the Need for Surgery in Horses with Colic.. Am. J. Vet. Res. 1991;51:1093–1907.
    doi: 10.2460/ajvr.1991.52.11.1903pubmed: 1785737google scholar: lookup
  6. Bishop R.C., Gutierrez-Nibeyro S.D., Stewart M.C., McCoy A.M.. Performance of Predictive Models of Survival in Horses Undergoing Emergency Exploratory Laparotomy for Colic.. Vet. Surg. 2022;51:891–902.
    doi: 10.1111/vsu.13839pmc: PMC9545965pubmed: 35674231google scholar: lookup
  7. Farrell A., Kersh K., Liepman R., Dembek K.A.. Development of a Colic Scoring System to Predict Outcome in Horses.. Front. Vet. Sci. 2021;8:697589.
    doi: 10.3389/fvets.2021.697589pmc: PMC8531487pubmed: 34692803google scholar: lookup
  8. Cummings C.O., Krucik D.D.R., Price E.. Clinical Predictive Models in Equine Medicine: A Systematic Review.. Equine Vet. J. 2023;55:573–583.
    doi: 10.1111/evj.13880pmc: PMC10073351pubmed: 36199162google scholar: lookup
  9. Furr M.O., Lessard P., White N.A.. Development of a Colic Severity Score for Predicting the Outcome of Equine Colic.. Vet. Surg. 1995;24:97–101.
    doi: 10.1111/j.1532-950X.1995.tb01302.xpubmed: 7778263google scholar: lookup
  10. Spadari A., Gialletti R., Gandini M., Valle E., Cerullo A., Cavallini D., Bertoletti A., Rinnovati R., Forni G., Scilimati N.. Short-Term Survival and Postoperative Complications Rates in Horses Undergoing Colic Surgery: A Multicentre Study.. Animals 2023;13:1107.
    doi: 10.3390/ani13061107pmc: PMC10044551pubmed: 36978647google scholar: lookup
  11. Ludwig E.K., Hobbs K.J., McKinney-Aguirre C.A., Gonzalez L.M.. Biomarkers of Intestinal Injury in Colic.. Animals 2023;13:227.
    doi: 10.3390/ani13020227pmc: PMC9854801pubmed: 36670767google scholar: lookup
  12. Radcliffe R.M., Divers T.J., Fletcher D.J., Mohammed H., Kraus M.S.. Evaluation of L-Lactate and Cardiac Troponin I in Horses Undergoing Emergency Abdominal Surgery.. J. Vet. Emerg. Crit. Care. 2012;22:313–319.
    doi: 10.1111/j.1476-4431.2012.00744.xpubmed: 22702437google scholar: lookup
  13. Johnston K., Holcombe S.J., Hauptman J.G.. Plasma Lactate as a Predictor of Colonic Viability and Survival after 360° Volvulus of the Ascending Colon in Horses.. Vet. Surg. 2007;36:563–567.
    doi: 10.1111/j.1532-950X.2007.00305.xpubmed: 17686130google scholar: lookup
  14. McCoy A.M., Hackett E.S., Wagner A.E., Mama K.R., Hendrickson D.A.. Pulmonary Gas Exchange and Plasma Lactate in Horses with Gastrointestinal Disease Undergoing Emergency Exploratory Laparotomy: A Comparison with an Elective Surgery Horse Population.. Vet. Surg. 2011;40:601–609.
    doi: 10.1111/j.1532-950X.2011.00840.xpubmed: 21539581google scholar: lookup
  15. Delesalle C., Dewulf J., Lefebvre R., Schuurkes J., Proot J., Lefere L., Deprez P.. Determination of Lactate Concentrations in Blood Plasma and Peritoneal Fluid in Horses with Colic by an Accusport Analyzer.. J. Vet. Intern. Med. 2007;21:293–301.
    doi: 10.1111/j.1939-1676.2007.tb02963.xpubmed: 17427391google scholar: lookup
  16. Westerman T.L., Foster C.M., Tornquist S.J., Poulsen K.P.. Evaluation of Serum Amyloid A and Haptoglobin Concentrations as Prognostic Indicators for Horses with Colic.. J. Am. Vet. Med. Assoc. 2016;248:935–940.
    doi: 10.2460/javma.248.8.935pubmed: 27031421google scholar: lookup
  17. Krueger C.R., Ruple-Czerniak A., Hackett E.S.. Evaluation of Plasma Muscle Enzyme Activity as an Indicator of Lesion Characteristics and Prognosis in Horses Undergoing Celiotomy for Acute Gastrointestinal Pain.. BMC Vet. Res. 2014;10:S7.
    doi: 10.1186/1746-6148-10-S1-S7pmc: PMC4122891pubmed: 25237781google scholar: lookup
  18. Kilcoyne I., Nieto J.E., Dechant J.E.. Predictive Value of Plasma and Peritoneal Creatine Kinase in Horses with Strangulating Intestinal Lesions.. Vet. Surg. 2019;48:152–158.
    doi: 10.1111/vsu.13147pubmed: 30588637google scholar: lookup
  19. Nocera I., Bonelli F., Vitale V., Meucci V., Conte G., Jose-Cunilleras E., Gracia-Calvo L.A., Sgorbini M.. Evaluation of Plasmatic Procalcitonin in Healthy, and in Systemic Inflammatory Response Syndrome (SIRS) Negative or Positive Colic Horses.. Animals 2021;11:2015.
    doi: 10.3390/ani11072015pmc: PMC8300415pubmed: 34359143google scholar: lookup
  20. Kilcoyne I., Nieto J.E., Dechant J.E.. Diagnostic Value of Plasma and Peritoneal Fluid Procalcitonin Concentrations in Horses with Strangulating Intestinal Lesions.. J. Am. Vet. Med. Assoc. 2020;256:927–933.
    doi: 10.2460/javma.256.8.927pubmed: 32223710google scholar: lookup
  21. Epstein A., Nir E., Eyngor M., Eldar A., Bdolah-Abram T., Kelmer E., Steinman A., Bruchim Y.. Dynamic of Cytokine Gene Transcription (TNF-α, IL-1β, IL-6, IL-8) in Surgically Treated Colic Horses by Use of Real-Time PCR (RT-PCR). Isr. J. Vet. Med. 2016;71:24–30.
  22. Barton M.H., Collatos C.. Tumor Necrosis Factor and Interleukin-6 Activity and Endotoxin Concentration in Peritoneal Fluid and Blood of Horses with Acute Abdominal Disease.. J. Vet. Intern. Med. 1999;13:457–464.
    doi: 10.1111/j.1939-1676.1999.tb01463.xpubmed: 10499730google scholar: lookup
  23. Steverink P.J.G.M., Sturk A., Rutten V.P.M.G., Wagenaar-Hilbers J.P.A., Klein W.R., Van Der Velden M.A., Németh F., Nemeth F.. Endotoxin, Interleukin-6 and Tumor Necrosis Factor Concentrations in Equine Acute Abdominal Disease: Relation to Clinical Outcome.. J. Endotoxin Res. 1995;2:289–299.
    doi: 10.1177/096805199500200409google scholar: lookup
  24. Latson K.M., Nieto J.E., Beldomenico P.M., Snyder J.R.. Evaluation of Peritoneal Fluid Lactate as a Marker of Intestinal Ischaemia in Equine Colic.. Equine Vet. J. 2005;37:342–346.
    doi: 10.2746/0425164054529319pubmed: 16028624google scholar: lookup
  25. Peloso J.G., Cohen N.D.. Use of Serial Measurements of Peritoneal Fluid Lactate Concentration to Identify Strangulating Intestinal Lesions in Referred Horses with Signs of Colic.. J. Am. Vet. Med. Assoc. 2012;240:1208–1217.
    doi: 10.2460/javma.240.10.1208pubmed: 22559111google scholar: lookup
  26. Vandenplas M.L., Moore J.N., Barton M.H., Ruossel A.J., Cohen N.D.. Concentrations of Serum Amyloid A and Lipopolysaccharide-Binding Protein in Horses with Colic.. Am. J. Vet. Res. 2005;66:1509–1516.
    doi: 10.2460/ajvr.2005.66.1509pubmed: 16261823google scholar: lookup
  27. Copas V.E.N., Durham A.E., Stratford C.H., McGorum B.C., Waggett B., Pirie R.S.. In Equine Grass Sickness, Serum Amyloid A and Fibrinogen Are Elevated, and Can Aid Differential Diagnosis from Non-Inflammatory Causes of Colic.. Vet. Rec. 2013;172:395.
    doi: 10.1136/vr.101224pubmed: 23428423google scholar: lookup
  28. Daniel A.J., Leise B.S., Burgess B.A., Morley P.S., Cloninger M., Hassel D.M.. Concentrations of Serum Amyloid A and Plasma Fibrinogen in Horses Undergoing Emergency Abdominal Surgery.. J. Vet. Emerg. Crit. Care. 2016;26:344–351.
    doi: 10.1111/vec.12365pubmed: 26274017google scholar: lookup
  29. Dondi F., Lukacs R.M., Gentilini F., Rinnovati R., Spadari A., Romagnoli N.. Serum Amyloid A, Haptoglobin, and Ferritin in Horses with Colic: Association with Common Clinicopathological Variables and Short-Term Outcome.. Vet. J. 2015;205:50–55.
    doi: 10.1016/j.tvjl.2015.03.015pubmed: 25981935google scholar: lookup
  30. Aucamp J., Bronkhorst A.J., Badenhorst C.P.S., Pretorius P.J.. The Diverse Origins of Circulating Cell-Free DNA in the Human Body: A Critical Re-Evaluation of the Literature.. Biol. Rev. 2018;93:1649–1683.
    doi: 10.1111/brv.12413pubmed: 29654714google scholar: lookup
  31. Yu D., Tong Y., Guo X., Feng L., Jiang Z., Ying S., Jia J., Fang Y., Yu M., Xia H.. Diagnostic Value of Concentration of Circulating Cell-Free DNA in Breast Cancer: A Meta-Analysis.. Front. Oncol. 2019;9:95.
    doi: 10.3389/fonc.2019.00095pmc: PMC6405437pubmed: 30881916google scholar: lookup
  32. Okajima W., Komatsu S., Ichikawa D., Miyamae M., Ohashi T., Imamura T., Kiuchi J., Nishibeppu K., Arita T., Konishi H.. Liquid Biopsy in Patients with Hepatocellular Carcinoma: Circulating Tumor Cells and Cell-Free Nucleic Acids.. World J. Gastroenterol. 2017;23:5650–5668.
    doi: 10.3748/wjg.v23.i31.5650pmc: PMC5569280pubmed: 28883691google scholar: lookup
  33. Jamal-Hanjani M., Wilson G.A., Horswell S., Mitter R., Sakarya O., Constantin T., Salari R., Kirkizlar E., Sigurjonsson S., Pelham R.. Detection of Ubiquitous and Heterogeneous Mutations in Cell-Free DNA from Patients with Early-Stage Non-Small-Cell Lung Cancer.. Ann. Oncol. 2016;27:862–867.
    doi: 10.1093/annonc/mdw037pubmed: 26823523google scholar: lookup
  34. Gil M.M., Accurti V., Santacruz B., Plana M.N., Nicolaides K.H.. Analysis of Cell-Free DNA in Maternal Blood in Screening for Aneuploidies: Updated Meta-Analysis.. Ultrasound Obstet. Gynecol. 2017;50:302–314.
    doi: 10.1002/uog.17484pubmed: 28397325google scholar: lookup
  35. Burnham P., Khush K., De Vlaminck I.. Myriad Applications of Circulating Cell-Free DNA in Precision Organ Transplant Monitoring.. Ann. Am. Thorac. Soc. 2017;14:S237–S241.
    doi: 10.1513/AnnalsATS.201608-634MGpmc: PMC5711344pubmed: 28945480google scholar: lookup
  36. Oellerich M., Schütz E., Beck J., Kanzow P., Plowman P., Weiss G., Walson P.. Using Circulating Cell-Free DNA to Monitor Personalized Cancer Therapy.. Crit. Rev. Clin. Lab. Sci. 2017;54:205–218.
    doi: 10.1080/10408363.2017.1299683pubmed: 28393575google scholar: lookup
  37. Naumann D.N., Hazeldine J., Dinsdale R.J., Bishop J.R., Midwinter M.J., Harrison P., Hutchings S.D., Lord J.M.. Endotheliopathy Is Associated with Higher Levels of Cell-Free DNA Following Major Trauma: A Prospective Observational Study.. PLoS ONE 2017;12:e0189870.
    doi: 10.1371/journal.pone.0189870pmc: PMC5736230pubmed: 29261771google scholar: lookup
  38. Avriel A., Wiessman M.P., Almog Y., Perl Y., Novack V., Galante O., Klein M., Pencina M.J., Douvdevani A.. Admission Cell Free DNA Levels Predict 28-Day Mortality in Patients with Severe Sepsis in Intensive Care.. PLoS ONE 2014;9:e100514.
    doi: 10.1371/journal.pone.0100514pmc: PMC4067333pubmed: 24955978google scholar: lookup
  39. Dwivedi D.J., Toltl L.J., Swystun L.L., Pogue J., Liaw K.L., Weitz J.I., Cook D.J., Fox-Robichaud A.E., Liaw P.C.. Prognostic Utility and Characterization of Cell-Free DNA in Patients with Severe Sepsis.. Crit. Care. 2012;16:R151.
    doi: 10.1186/cc11466pmc: PMC3580740pubmed: 22889177google scholar: lookup
  40. Rainer T.H., Chan A.K.C., Lee L.L.Y., Yim V.W.T., Lam N.Y.L., Yeung S.W., Graham C.A., Lo D.Y.M.. Use of Plasma DNA to Predict Mortality and Need for Intensive Care in Patients with Abdominal Pain.. Clin. Chim. Acta. 2008;398:113–117.
    doi: 10.1016/j.cca.2008.08.022pubmed: 18801348google scholar: lookup
  41. Arnalich F., Maldifassi M.C., Ciria E., Quesada A., Codoceo R., Herruzo R., Garcia-Cerrada C., Montoya F., Vazquez J.J., López-Collazo E.. Association of Cell-Free Plasma DNA with Perioperative Mortality in Patients with Suspected Acute Mesenteric Ischemia.. Clin. Chim. Acta. 2010;411:1269–1274.
    doi: 10.1016/j.cca.2010.05.017pubmed: 20478285google scholar: lookup
  42. Letendre J.-A., Goggs R.. Measurement of Plasma Cell-Free DNA Concentrations in Dogs with Sepsis, Trauma, and Neoplasia.. J. Vet. Emerg. Crit. Care. 2017;27:307–314.
    doi: 10.1111/vec.12592pubmed: 28295988google scholar: lookup
  43. Letendre J.-A., Goggs R.. Determining Prognosis in Canine Sepsis by Bedside Measurement of Cell-Free DNA and Nucleosomes.. J. Vet. Emerg. Crit. Care. 2018;28:503–511.
    doi: 10.1111/vec.12773pubmed: 30299568google scholar: lookup
  44. Letendre J.-A., Goggs R.. Concentrations of Plasma Nucleosomes but Not Cell-Free DNA Are Prognostic in Dogs Following Trauma.. Front. Vet. Sci. 2018;5:180.
    doi: 10.3389/fvets.2018.00180pmc: PMC6077184pubmed: 30105230google scholar: lookup
  45. Jeffery U., Ruterbories L., Hanel R., LeVine D.N.. Cell-Free DNA and DNase Activity in Dogs with Immune-Mediated Hemolytic Anemia.. J. Vet. Intern. Med. 2017;31:1441–1450.
    doi: 10.1111/jvim.14808pmc: PMC5598899pubmed: 28833583google scholar: lookup
  46. Troia R., Giunti M., Calipa S., Goggs R.. Cell-Free DNA, High-Mobility Group Box-1, and Procalcitonin Concentrations in Dogs with Gastric Dilatation–Volvulus Syndrome.. Front. Vet. Sci. 2018;5:67.
    doi: 10.3389/fvets.2018.00067pmc: PMC5900424pubmed: 29686994google scholar: lookup
  47. Fingerhut L., Ohnesorge B., von Borstel M., Schumski A., Strutzberg-Minder K., Mörgelin M., Deeg C.A., Haagsman H.P., Beineke A., von Köckritz-Blickwede M.. Neutrophil Extracellular Traps in the Pathogenesis of Equine Recurrent Uveitis (ERU). Cells 2019;8:1528.
    doi: 10.3390/cells8121528pmc: PMC6953072pubmed: 31783639google scholar: lookup
  48. Colmer S.F., Luethy D., Abraham M., Stefanovski D., Hurcombe S.D.. Utility of Cell-Free DNA Concentrations and Illness Severity Scores to Predict Survival in Critically Ill Neonatal Foals.. PLoS ONE 2021;16:e0242635.
    doi: 10.1371/journal.pone.0242635pmc: PMC8075268pubmed: 33901192google scholar: lookup
  49. Panizzi L., Dittmer K.E., Vignes M., Doucet J.S., Gedye K., Waterland M.R., Rogers C.W., Sano H., McIlwraith C.W., Riley C.B.. Plasma and Synovial Fluid Cell-Free DNA Concentrations Following Induction of Osteoarthritis in Horses.. Animals 2023;13:1053.
    doi: 10.3390/ani13061053pmc: PMC10044647pubmed: 36978592google scholar: lookup
  50. Bayless R.L., Cooper B.L., Sheats M.K.. Investigation of Plasma Cell-Free DNA as a Potential Biomarker in Horses.. J. Vet. Diagn. Investig. 2022;34:402–406.
    doi: 10.1177/10406387221078047pmc: PMC9254060pubmed: 35168428google scholar: lookup
  51. Burnett D.L., Cave N.J., Gedye K.R., Bridges J.P.. Investigation of Cell-Free DNA in Canine Plasma and Its Relation to Disease.. Vet. Q. 2016;36:122–129.
    doi: 10.1080/01652176.2016.1182230pubmed: 27103480google scholar: lookup
  52. Forsblom E., Aittoniemi J., Ruotsalainen E., Helmijoki V., Huttunen R., Jylhävä J., Hurme M., Järvinen A.. High Cell-Free DNA Predicts Fatal Outcome among Staphylococcus Aureus Bacteraemia Patients with Intensive Care Unit Treatment.. PLoS ONE 2014;9:e87741.
    doi: 10.1371/journal.pone.0087741pmc: PMC3919733pubmed: 24520336google scholar: lookup
  53. Goggs R.. Effect of Sample Type on Plasma Concentrations of Cell-Free DNA and Nucleosomes in Dogs.. Vet. Rec. Open. 2019;6:e000357.
    doi: 10.1136/vetreco-2019-000357pmc: PMC6802997pubmed: 31673376google scholar: lookup
  54. Wilson I.J., Burchell R.K., Worth A.J., Burton S.E., Gedye K.R., Clark K.J., Crosse K.R., Jack M., Odom T.F., De Grey S.J.. Kinetics of Plasma Cell-Free DNA and Creatine Kinase in a Canine Model of Tissue Injury.. J. Vet. Intern. Med. 2018;32:157–164.
    doi: 10.1111/jvim.14901pmc: PMC5787206pubmed: 29230875google scholar: lookup
  55. Huttunen R., Kuparinen T., Jylhävä J., Aittoniemi J., Vuento R., Huhtala H., Laine J., Syrjänen J., Hurme M.. Fatal Outcome in Bacteremia Is Characterized by High Plasma Cell Free DNA Concentration and Apoptotic DNA Fragmentation: A Prospective Cohort Study.. PLoS ONE 2011;6:e21700.
    doi: 10.1371/journal.pone.0021700pmc: PMC3128600pubmed: 21747948google scholar: lookup
  56. Hobbs K.J., Cooper B.L., Dembek K., Sheats M.K.. Investigation of Extracted Plasma Cell-Free DNA as a Biomarker in Foals with Sepsis.. Vet. Sci. 2024;11:346.
    doi: 10.3390/vetsci11080346pmc: PMC11359113pubmed: 39195800google scholar: lookup
  57. Saukkonen K., Lakkisto P., Pettilä V., Varpula M., Karlsson S., Ruokonen E., Pulkki K.. Cell-Free Plasma DNA as a Predictor of Outcome in Severe Sepsis and Septic Shock.. Clin. Chem. 2008;54:1000–1007.
    doi: 10.1373/clinchem.2007.101030pubmed: 18420731google scholar: lookup
  58. Xu Y., Song Y., Chang J., Zhou X., Qi Q., Tian X., Li M., Zeng X., Xu M., Zhang W.. High Levels of Circulating Cell-Free DNA Are a Biomarker of Active SLE.. Eur. J. Clin. Investig. 2018;48:e13015.
    doi: 10.1111/eci.13015pubmed: 30079480google scholar: lookup
  59. Roy M.-F., Kwong G.P.S., Lambert J., Massie S., Lockhart S.. Prognostic Value and Development of a Scoring System in Horses with Systemic Inflammatory Response Syndrome.. J. Vet. Intern. Med. 2017;31:582–592.
    doi: 10.1111/jvim.14670pmc: PMC5354005pubmed: 28207163google scholar: lookup
  60. Barrett A.N., Thadani H.A., Laureano-Asibal C., Ponnusamy S., Choolani M.. Stability of Cell-Free DNA from Maternal Plasma Isolated Following a Single Centrifugation Step.. Prenat. Diagn. 2014;34:1283–1288.
    doi: 10.1002/pd.4468pubmed: 25066782google scholar: lookup
  61. Jung M., Klotzek S., Lewandowski M., Fleischhacker M., Jung K.. Changes in Concentration of DNA in Serum and Plasma during Storage of Blood Samples.. Clin. Chem. 2003;49:1028–1029.
    doi: 10.1373/49.6.1028pubmed: 12766024google scholar: lookup
  62. Sato A., Nakashima C., Abe T., Kato J., Hirai M., Nakamura T., Komiya K., Kimura S., Sueoka E., Sueoka-Aragane N.. Investigation of Appropriate Pre-Analytical Procedure for Circulating Free DNA from Liquid Biopsy.. Oncotarget 2018;9:31904–31914.
    doi: 10.18632/oncotarget.25881pmc: PMC6112748pubmed: 30159131google scholar: lookup
  63. Smith S.A., Lawson C.M., McMichael M.A., Jung K., O’Brien M., Achiel R.. Evaluation of Assays for Quantification of DNA in Canine Plasma as an Indirect Marker of NETosis.. Vet. Clin. Pathol. 2017;46:278–286.
    doi: 10.1111/vcp.12478pubmed: 28370080google scholar: lookup
  64. Rather R.A., Saha S.C., Dhawan V.. The Most Favourable Procedure for the Isolation of Cell-Free DNA from the Plasma of Iso-Immunized RHD-Negative Pregnant Women.. J. Circ. Biomark. 2015;4:12.
    doi: 10.5772/62113pmc: PMC5548194pubmed: 28936248google scholar: lookup
  65. Kloten V., Rüchel N., Brüchle N.O., Gasthaus J., Freudenmacher N., Steib F., Mijnes J., Eschenbruch J., Binnebösel M., Knüchel R.. Liquid Biopsy in Colon Cancer: Comparison of Different Circulating DNA Extraction Systems Following Absolute Quantification of KRAS Mutations Using Intplex Allele-Specific PCR.. Oncotarget 2017;8:86253–86263.
    doi: 10.18632/oncotarget.21134pmc: PMC5689682pubmed: 29156792google scholar: lookup
  66. Ponti G., Maccaferri M., Manfredini M., Kaleci S., Mandrioli M., Pellacani G., Ozben T., Depenni R., Bianchi G., Pirola G.M.. The Value of Fluorimetry (Qubit) and Spectrophotometry (NanoDrop) in the Quantification of Cell-Free DNA (CfDNA) in Malignant Melanoma and Prostate Cancer Patients.. Clin. Chim. Acta. 2018;479:14–19.
    doi: 10.1016/j.cca.2018.01.007pubmed: 29309771google scholar: lookup
  67. Parackal S., Zou D., Day R., Black M., Guilford P.. Comparison of Roche Cell-Free DNA Collection Tubes® to Streck Cell-Free DNA BCT®s for Sample Stability Using Healthy Volunteers.. Pract. Lab. Med. 2019;16:e00125.
    doi: 10.1016/j.plabm.2019.e00125pmc: PMC6593214pubmed: 31289732google scholar: lookup
  68. Nikolaev S., Lemmens L., Koessler T., Blouin J.L., Nouspikel T.. Circulating Tumoral DNA: Preanalytical Validation and Quality Control in a Diagnostic Laboratory.. Anal. Biochem. 2018;542:34–39.
    doi: 10.1016/j.ab.2017.11.004pubmed: 29137972google scholar: lookup
  69. Pérez-Barrios C., Nieto-Alcolado I., Torrente M., Jiménez-Sánchez C., Calvo V., Gutierrez-Sanz L., Palka M., Donoso-Navarro E., Provencio M., Romero A.. Comparison of Methods for Circulating Cell-Free DNA Isolation Using Blood from Cancer Patients: Impact on Biomarker Testing.. Transl. Lung Cancer Res. 2016;5:665–672.
    doi: 10.21037/tlcr.2016.12.03pmc: PMC5233878pubmed: 28149760google scholar: lookup
  70. Jackson Chornenki N.L., Coke R., Kwong A.C., Dwivedi D.J., Xu M.K., McDonald E., Marshall J.C., Fox-Robichaud A.E., Charbonney E., Liaw P.C.. Comparison of the Source and Prognostic Utility of CfDNA in Trauma and Sepsis.. Intensive Care Med. Exp. 2019;7:29.
    doi: 10.1186/s40635-019-0251-4pmc: PMC6531595pubmed: 31119471google scholar: lookup
  71. Fridlich O., Peretz A., Fox-Fisher I., Pyanzin S., Dadon Z., Shcolnik E., Sadeh R., Fialkoff G., Sharkia I., Moss J.. Elevated CfDNA after Exercise Is Derived Primarily from Mature Polymorphonuclear Neutrophils, with a Minor Contribution of Cardiomyocytes.. Cell Rep. Med. 2023;4:101074.
    doi: 10.1016/j.xcrm.2023.101074pmc: PMC10313937pubmed: 37290439google scholar: lookup
  72. Juškevičiūtė E., Neuberger E., Eimantas N., Heinkel K., Simon P., Brazaitis M.. Cell-Free DNA Kinetics in Response to Muscle-Damaging Exercise: A Drop Jump Study.. Exp. Physiol. 2024;109:1341–1352.
    doi: 10.1113/EP091986pmc: PMC11291858pubmed: 38875105google scholar: lookup
  73. Andreatta M.V., Curty V.M., Coutinho J.V.S., Santos M.Â.A., Vassallo P.F., de Sousa N.F., Barauna V.G.. Cell-Free DNA as an Earlier Predictor of Exercise-Induced Performance Decrement Related to Muscle Damage.. Int. J. Sports Physiol. Perform. 2018;13:953–956.
    doi: 10.1123/ijspp.2017-0421pubmed: 29182414google scholar: lookup
  74. Hunt H., Cave N., Bridges J., Gedye K., Hill K.. Plasma NT-ProBNP and Cell-Free DNA Concentrations after Prolonged Strenuous Exercise in Working Farm Dogs.. J. Vet. Intern. Med. 2018;32:135–141.
    doi: 10.1111/jvim.14835pmc: PMC5787186pubmed: 29197094google scholar: lookup
  75. Devall V.C., Goggs R., Hansen C., Frye C.W., Letendre J.A., Wakshlag J.J.. Serum Myoglobin, Creatine Kinase, and Cell-Free DNA in Endurance Sled Dogs and Sled Dogs with Clinical Rhabdomyolysis.. J. Vet. Emerg. Crit. Care. 2018;28:310–316.
    doi: 10.1111/vec.12731pubmed: 29898248google scholar: lookup

Citations

This article has been cited 0 times.
Subscribe to our newsletter
Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.