Extraction of equine chorionic gonadotrophin from pregnant mare plasma by direct adsorption on chromatographic media.

This research paper presents a new method for extracting the equine chorionic gonadotrophin (eCG) hormone from pregnant mare blood plasma, detailing the process’s high recovery rate, preservation of biological activity, and applicability on an industrial scale.

Introduction to Equine Chorionic Gonadotrophin (eCG)

• Equine chorionic gonadotrophin (eCG) is a hormone that has found significant importance in veterinary medicine and animal production.

New Preparation Procedure

• The researchers propose a novel method to isolate eCG based on its direct adsorption on anionic-exchange resins.
• This method involves conditioning the active plasma to reduce its pH and ionic strength. After these modifications, the plasma is suitable for the adsorption stage where eCG binds to the anionic-exchange resins.

Results from the Adsorption Stage

• The adsorption stage results in approximately 90% recovery of eCG from the initial plasma sample.
• The concentration and enrichment factors of the recovery are about 50 and 500, respectively, indicating a successful procedure that increases the availability of eCG while maintaining its biological activity.

Further Purification

• After the adsorption process, the next step involves further purification via cation-exchange column chromatography.
• This secondary stage of the process results in a recovery rate exceeding 70% for the whole process, further illustrating the efficiency of the new preparation procedure.

Preparation Potency and Scalability

• The final result of this process yields an eCG preparation with a potency close to 4000 IU/mg.
• Notably, this technique is also ideal for industrial-scale applications due to its high yield and efficiency, making it a practical method for large-scale eCG extraction.