Eye Surface Temperature as a Potential Indicator of Physical Fitness in Ranch Horses.

This research studied the possibility of using maximum eye surface temperature (EST) as a gauge of physical fitness in ranch horses. Six fitness markers were recorded before and after standard working conditions, and the difference in EST was analyzed alongside these markers. Findings suggest that changes in EST may indicate a horse’s physical fitness, particularly changes in muscle damage, although further research is required.

Methodology

• The researchers collected data from 16 horses, recording six fitness biomarkers which include eye surface temperature (EST), respiratory rate, creatine kinase activity (CK), total serum protein concentration, plasma cortisol and lactate concentrations.
• The heart rate all throughout the workout, the exercise duration, the speed, the distance, and the air temperature and humidity were all logged.
• In order to compare the variation between before and after measurements, the scientists used repeated measures ANOVA or the paired Wilcoxon test. Also, the t-test was employed to compare the EST means of dehydrated and non-dehydrated horses.

Findings

• All biomarkers, including EST, amplified after the work day. Intriguingly, the average EST remained similar between dehydrated and non-dehydrated horses.
• CK and EST were found to be positively connected – an increase in one generally indicated an increase in the other.
• Differences between each variable’s sampling times were calculated and a correlation analysis was carried out, showing clear correlation between Differential-CK and Differential-EST.

Cluster Analysis

• A hierarchical cluster analysis sorted the horses into two groups – LOW and HIGH – based on their physical fitness.
• The HIGH horses showed CK levels greater than 350 U/L, indicating muscle damage to some extent.
• Statistical differences were found between these two groups in terms of Differential-EST and Differential-CK.

Conclusion

• The study’s results point to the possible use of EST as a predictor for changes in CK, which could be instrumental in assessing the physical fitness of ranch horses.
• However, additional research is necessary to confirm the reliability of this potential fitness indicator.