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Feasibility of hemoperfusion using extracorporeal therapy in the horse.
Abstract: Develop, implement, and monitor for adverse effects of, a novel hemoperfusion therapy in adult horses. Unassigned: A prospective, observational feasibility study using three healthy adult horses from the North Carolina State University teaching herd. Health status was determined by physical exam, complete blood count, coagulation panel, and serum biochemistry. Each horse was instrumented with a 14 Fr × 25 cm double-lumen temporary hemodialysis catheter and underwent a 240 min polymer-based hemoperfusion session. Horses were administered unfractionated heparin to maintain anti-coagulation during the session. Given the novelty of this therapy in horses, each horse was treated as a learning opportunity that informed an iterative process of protocol development and modification. Unassigned: Our long-term goal is to investigate potential clinical applications of hemoperfusion in horses, including cytokine reduction in horses with severe SIRS/sepsis. Horses were monitored for changes in clinical exam, biochemistry and hematology parameters. Additionally, cytokines were quantified to determine whether extracorporeal hemadsorption therapy alone caused an inflammatory response. Our results show that hemoperfusion therapy was associated with decreased platelet counts and serum albumin concentration. There was no significant change in plasma cytokine concentrations with hemoperfusion therapy. In one horse, the cytokine concentrations decreased, as previously reported with hemoperfusion therapy in humans. Unassigned: We hypothesized that hemoperfusion therapy could be performed in healthy adult horses without significant adverse effects. Unassigned: Polymer-based hemoperfusion is a feasible extracorporeal therapy (ECT) modality for adult horses. Additional studies are needed to further establish clinical protocols, as well as establish efficacy of polymer-based hemoperfusion for treatment of various conditions in horses, including intoxications, immune-mediated conditions, and sepsis.
Copyright © 2024 Hobbs, Le Sueur, Burke, Cooper, Sheats and Ueda.
Publication Date: 2024-05-14 PubMed ID: 38803798PubMed Central: PMC11128652DOI: 10.3389/fvets.2024.1414426Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This is a preliminary research study about the feasibility of hemoperfusion therapy in adult horses. The research indicates that this therapy is possible without significant adverse effects, although it did affect platelet count and albumin concentration in the horse’s serum. The therapy might potentially be useful for treating diseases like sepsis in animals.
Overview of the Research
- The study aims to develop, put into practice, and assess the side effects of a hemoperfusion treatment in adult horses. The therapy is quite innovative in equine medicine.
- The research was conducted using three healthy adult horses from the North Carolina State University teaching herd.
- Each horse underwent a thorough physical examination, complete blood count, coagulation panel, and serum biochemistry to confirm its health status.
Hemoperfusion Therapy Procedure
- Each horse was equipped with a 14 Fr × 25 cm double-lumen temporary hemodialysis catheter and went through a 240-minute polymer-based hemoperfusion session.
- Unfractionated heparin was employed in each session to maintain anti-coagulation throughout the treatment.
- Every horse was treated as a unique case, allowing the researchers to continuously improve the protocol based on the experiences and outcomes of the animals.
Monitoring & Results of Hemoperfusion Therapy
- The horses were keenly observed for changes in clinical exam results, biochemistry parameters, and hematology parameters.
- In addition, the study checked for inflammatory response by quantifying cytokines to determine whether extracorporeal hemadsorption therapy alone incited an inflammatory response.
- The results revealed a decrease in platelet counts and serum albumin concentration. This decrement might be because of the therapy itself.
- The plasma cytokine concentrations didn’t show any significant changes post hemoperfusion therapy in healthy horses. However, the therapy managed to lower cytokine concentrations in one horse, mimicking the results observed in human treatments.
Conclusions and Future Directions
- The goal of this study was to examine whether hemoperfusion therapy can be safely administered to healthy adult horses. The results seem to suggest that it can.
- In conclusion, this research suggests that polymer-based hemoperfusion is a promising extracorporeal therapy (ECT) procedure for adult horses.
- More expansive studies are crucial to solidify the clinical protocols, and to establish the efficiency of polymer-based hemoperfusion for treating various conditions in horses, including intoxications, immune-mediated conditions, and sepsis.
Cite This Article
APA
Hobbs KJ, Le Sueur ANV, Burke MJ, Cooper BL, Sheats MK, Ueda Y.
(2024).
Feasibility of hemoperfusion using extracorporeal therapy in the horse.
Front Vet Sci, 11, 1414426.
https://doi.org/10.3389/fvets.2024.1414426 Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.
- Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.
- Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.
- Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.
- Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.
- Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Citations
This article has been cited 2 times.- Hobbs KJ, Bayless R, Sheats MK. A Comparative Review of Cytokines and Cytokine Targeting in Sepsis: From Humans to Horses. Cells 2024 Sep 5;13(17).
- Hobbs KJ, Le Sueur ANV, Hallowell K, Martin E, Sheats MK, Ueda Y. Use of extracorporeal hemoperfusion therapy in an adult horse with Clostridioides difficile colitis and severe systemic inflammatory response syndrome. J Vet Intern Med 2024 Sep-Oct;38(5):2790-2794.
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